Abstract:
:A nonradioactive functional assay was developed to quantitate DNA binding proteins. The assay was designed to allow the use of 96-well microplates for high sample throughput. We show that the assay can measure sequence-specific DNA binding of purified proteins as well as DNA binding activity present in whole cell extracts. By using a temperature-sensitive DNA binding protein, we demonstrate that DNA binding as measured by this novel assay correlates with the biological function of the protein.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Gubler ML,Abarzúa Psubject
Has Abstractpub_date
1995-06-01 00:00:00pages
1008, 1011-4issue
6eissn
0736-6205issn
1940-9818journal_volume
18pub_type
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