Abstract：
:Gene expression studies require analysis of RNA, but isolation of total RNA from very small samples by traditional methods can be difficult and inefficient. The Absolutely RNA microprep kit provides a convenient method for isolating total RNA from small numbers of cells such as those harvested by laser capture microdissection (LCM). The protocol includes binding of RNA to a solid support, thus eliminating the need for organic extraction and alcohol precipitation. DNase digestion on the solid support reduces or eliminates DNA contamination and minimizes RNA handling. Efficient washing removes contaminants, and elution in a small volume of buffer results in high-purity RNA at a concentration appropriate for demanding applications such as RT-PCR. RNA isolated from as few as 200 laser capture microdissected brain tumor cells resulted in detection of low, medium, and highly expressed genes by conventional and real-time RT-PCR.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Dolter KE,Braman JCdoi
10.2144/01306pf01subject
Has Abstractpub_date
2001-06-01 00:00:00pages
1358-61issue
6eissn
0736-6205issn
1940-9818journal_volume
30pub_type
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