Abstract:
:Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: martin.kennedy@otago.ac.nz.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Stevens AJ,Appleby S,Kennedy MAdoi
10.2144/000114481subject
Has Abstractpub_date
2016-12-01 00:00:00pages
293-296issue
6eissn
0736-6205issn
1940-9818pii
000114481journal_volume
61pub_type
信件相关文献
BIOTECHNIQUES文献大全abstract::Blue/white selection is the standard method for detecting a cloned DNA fragment. In the absence of an insert, uninterrupted expression of the vector-encoded alpha-complement of beta-galactosidase (beta-gal), results in the hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactoside) and the subsequent blue stai...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/01305pf01
更新日期:2001-05-01 00:00:00
abstract::For immunological research on the human cytomegalovirus (HCMV), a virus that combines the broad cell tropism of clinical isolates, efficient replication in cell culture, the complete set of MHC-I modulator genes, and suitability for genetic engineering is desired. Here, we aimed to generate a genetically complete deri...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000114606
更新日期:2017-11-01 00:00:00
abstract::Normalization is a critical step in the analysis of microarray gene expression data. For dual-labeled array, traditional normalization methods assume that the majority of genes are non-differentially expressed and that the number of overexpressed genes approximately equals the number of under-expressed genes. However,...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113195
更新日期:2009-08-01 00:00:00
abstract::In this report we present a rapid and inexpensive PCR-based method to screen recombinant DNA libraries. The efficiency of this method was demonstrated by the isolation of clones of interest from three different libraries using different vector systems. This method is nonradioactive and makes it easier to handle a larg...
journal_title:BioTechniques
pub_type:
doi:
更新日期:1994-01-01 00:00:00
abstract::Address correspondence to Mart Speek, Department of Gene Technology, Akadeemia tee 15, Room 129, Tallinn University of Technology, Tallinn 19086, Estonia. E-mail: mart.speek@ttu.ee. ...
journal_title:BioTechniques
pub_type: 信件
doi:10.2144/000114441
更新日期:2016-08-01 00:00:00
abstract::S-100B is used in melanoma follow-up. This serum biomarker is also present in adipocytes; therefore, subcutaneous adipocytes trapped in the needle before performing a venipuncture could contaminate the serum. The aim was to study the influence of adipocyte contamination on blood samples used for S-100B analysis, possi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0147
更新日期:2020-11-01 00:00:00
abstract::Intrinsically disordered proteins (IDPs) are subject to post-translational modifications. This allows the same polypeptide to be involved in different interaction networks with different consequences, ranging from regulatory signalling networks to the formation of membrane-less organelles. We report a robust method fo...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2019-0033
更新日期:2019-07-01 00:00:00
abstract::A nascent protein remains in a complex with its ribosome and mRNA if the stop codon is deleted from the mRNA. In the same manner, mRNA forms a stable complex with DNA if the transcription termination is blocked. In principle, if both mRNA translation and DNA transcription termination are prevented, the protein should ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/00294st06
更新日期:2000-10-01 00:00:00
abstract::Naturstoff reagent A (diphenylboric acid 2-aminoethyl ester [DPBA]) has been used historically in plant science to observe polyphenolic pigments, such as flavonoids, whose fluorescence requires enhancement to be visible by microscopy. Flavonoids are common dietary constituents and are the focus of considerable attenti...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/btn-2018-0084
更新日期:2019-02-01 00:00:00
abstract::We describe a rapid, simple and inexpensive method for the isolation of DNA from blood clots suited for use in PCR. Our method is based on the lysing and nuclease-inactivating properties of guanidine thiocyanate together with the nucleic acid-binding properties of silica particles. Isolated DNA can be used for in vitr...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted a...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113738
更新日期:2011-09-01 00:00:00
abstract::A PCR-based method is described for the production of cDNA libraries and total cDNA probes from a few milligrams of tissue. Using a model system, we show how a PCR library and PCR probes can be used to identify genes expressed at different levels in two tissues. Small amounts of tissue derived from two plants, one inf...
journal_title:BioTechniques
pub_type:
doi:
更新日期:1994-06-01 00:00:00
abstract::To measure mutation load or to detect minimal residual disease, a robust method for identifying one mutant allele in the range of 10(6)-10(9) wild-type alleles would be advantageous. Herein, we present evidence that pyrophosphorolysis-activated polymerization (PAP) has the potential to provide a highly specific and ro...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/00295rr03
更新日期:2000-11-01 00:00:00
abstract::Single-stranded long oligonucleotide-based (50- to 70-mer) microarrays offer several advantages over conventional cDNA microarrays. These include the easy preparation of the probes, low cost of array production, and low cross-contamination during probe handling. However, the application of oligonucleotide microarrays ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/04374ST02
更新日期:2004-10-01 00:00:00
abstract::Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg wh...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113914
更新日期:2012-08-01 00:00:00
abstract::Phage display holds a key position in the use of combinatorial library approaches for the purpose of protein engineering and discovery. However, modifying the pIII protein of the phage can severely and negatively influence the infectiousness of the phage particle. This concern is particularly relevant when large pIII ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112724
更新日期:2008-04-01 00:00:00
abstract::Subtractive hybridization has been widely used for the identification of differentially expressed genes. Here we describe a simple, sensitive strategy of subtractive hybridization that involves binding the driver poly(A)+ RNA pool to paramagnetic Dynabeads Oligo (dT)25. After hybridization with target cDNA, the molecu...
journal_title:BioTechniques
pub_type:
doi:10.2144/19962003413
更新日期:1996-03-01 00:00:00
abstract::We have developed a rapid [3H]colchicine competition-binding scintillation proximity assay (SPA) to evaluate antimitotic compounds that bind to the colchicine-binding site on tubulin. The premise of our assay is that compounds will compete with radiolabeled colchicine for the tubulin-binding domain. Biotin-labeled tub...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/00291rr02
更新日期:2000-07-01 00:00:00
abstract::Directed evolution is poised to change small molecule discovery and provide greater access to "drug space." Sarah Webb looks into the evolving drug discovery landscape. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114616
更新日期:2017-12-01 00:00:00
abstract::The nematode Caenorhabditis elegans is an important model animal for biological research. Currently, transgenic C. elegans strains are mainly generated by injecting DNA encoding a gene of interest, in combination with a reporter gene, into the gonad. With this approach, the interpretation of negative results, such as ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113821
更新日期:2012-03-01 00:00:00
abstract::Changes in cardiac structure that depart from normal have generally been termed "remodeling". Assessment of ventricular remodeling at the cellular level should include measurement of myocyte dimensions. A well-established and reliable method to assess myocyte remodeling uses isolated cells and the Coulter Counter/Chan...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/98253pf02
更新日期:1998-09-01 00:00:00
abstract::Complete brain fixation can be achieved with transthoracic cardiac infusion without thoracotomy. Light and electron microscopy tissue sections reveal preservation of cytoplasmic and nuclear structure at all magnification levels. Punched samples were obtained from the fixed tissue specimens in precisely localized areas...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112003
更新日期:2005-10-01 00:00:00
abstract::Haplotypes are useful in population genetics and medicine. Determining haplotypes in the absence of DNA samples from appropriate family members can be difficult and laborious. We have developed a rapid and reproducible method for haplotyping an individual in the absence of relatives. The method utilizes pairs of allel...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1991-04-01 00:00:00
abstract::We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes. ...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Fluorescent dye terminator Sanger sequencing (FTSS), with detection by automated capillary electrophoresis (CE), has long been regarded as the gold standard for variant detection. However, software analysis and base-calling algorithms used to detect mutations were largely optimized for resequencing applications in whi...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000113913
更新日期:2012-09-01 00:00:00
abstract::Specific amplification of nucleic acid sequences by PCR has been extensively used for the detection of gene rearrangements and gene expression. Although successful amplification of DNA sequences has been carried out with DNA prepared from formalin-fixed, paraffin-embedded (FFPE) tissues, there are only a few reports r...
journal_title:BioTechniques
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Here we describe a convenient method to generate homologous recombinant baculoviral genomes in E. coli. The recombination takes place with the aid of recombination enzymes provided by the phage lambda Red system between a bacmid (a baculoviral genome that can replicate in bacteria) and a linear fragment. Proof of conc...
journal_title:BioTechniques
pub_type:
doi:10.2144/02324st04
更新日期:2002-04-01 00:00:00
abstract::Characterization of the thermodynamics of binding interactions is important in improving our understanding of bimolecular recognition and forms an essential part of the rational drug design process. Isothermal titration calorimetry (ITC) is rapidly becoming established as the method of choice for undertaking such stud...
journal_title:BioTechniques
pub_type: 杂志文章,评审
doi:
更新日期:2001-07-01 00:00:00
abstract::Rapid identification of viruses is needed to monitor the blood supply for emerging threats. Here we present a method that meets these criteria and allows for the shotgun sequencing of novel, uncultured DNA viruses directly from human blood. This method employs selection based on the physical properties of viruses comb...
journal_title:BioTechniques
pub_type: 杂志文章
doi:10.2144/000112019
更新日期:2005-11-01 00:00:00
abstract::A 200-year-old observation might provide a new way to eliminate tumors by infecting cancer patients with bacteria. Kristie Nybo explores a new approach that could transform cancer treatment. ...
journal_title:BioTechniques
pub_type: 新闻
doi:10.2144/000114624
更新日期:2018-01-01 00:00:00