Abstract:
:A PCR-based method is described for the production of cDNA libraries and total cDNA probes from a few milligrams of tissue. Using a model system, we show how a PCR library and PCR probes can be used to identify genes expressed at different levels in two tissues. Small amounts of tissue derived from two plants, one infected with arabis mosaic virus and the other uninfected, were used to make a library and probes. This library and the probes were used to identify viral genes expressed only in the infected plant.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Bertiol DJ,Smoker M,Brown AC,Jones MG,Burrows PRsubject
Has Abstractpub_date
1994-06-01 00:00:00pages
1054-8issue
6eissn
0736-6205issn
1940-9818journal_volume
16pub_type
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