Haplotyping by double PCR amplification of specific alleles.

Abstract:

:Haplotypes are useful in population genetics and medicine. Determining haplotypes in the absence of DNA samples from appropriate family members can be difficult and laborious. We have developed a rapid and reproducible method for haplotyping an individual in the absence of relatives. The method utilizes pairs of allele-specific PCR primers to differentially amplify each haplotype. Four amplifications can distinguish the haplotypes produced by two biallelic polymorphisms.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Sarkar G,Sommer SS

subject

Has Abstract

pub_date

1991-04-01 00:00:00

pages

436, 438, 440

issue

4

eissn

0736-6205

issn

1940-9818

journal_volume

10

pub_type

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