Abstract：
:We present a simple method for sequential chemiluminescent detections of two different DNA loci on a single Southern blot. First, an enzyme-linked DNA probe for a unique sequence is detected with a horse-radish peroxidase (HRP) substrate followed by the detection of another enzyme-linked DNA probe for a different unique sequence with an alkaline phosphatase (AP) substrate that simultaneously inhibits the chemiluminescence generated by HRP. Such sequential detection steps eliminate the need to strip and reprobe blots and can be performed with no intervening steps.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Reddy LV,DeSilva R,Handley RS,Schaap AP,Akhavan-Tafti Hdoi
10.2144/99264st08subject
Has Abstractpub_date
1999-04-01 00:00:00pages
710-4issue
4eissn
0736-6205issn
1940-9818journal_volume
26pub_type
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