Abstract：
:We propose two methods for characterizing the spatio-temporal behavior of cell populations in culture. The first method, image auto-correlation microscopy (IACM), allows us to characterize the variation in the number of objects as a function of time, thus enabling the quantification of the clustering properties of cell populations to be performed. The second method, image cross-correlation microscopy (ICCM), allows us to characterize the migration properties of cell populations. The latter method does not require estimation or measurement of the trajectories of individual cells, which is very demanding when populations of >100 cells are examined. The capabilities of the two methods are demonstrated with simulated cell populations, and their usefulness is illustrated with experiments involving invasive and noninvasive tumor cell populations.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Bonnet N,Delavoie F,Zahm JMdoi
10.2144/000112478subject
Has Abstractpub_date
2007-07-01 00:00:00pages
107-15issue
1eissn
0736-6205issn
1940-9818pii
000112478journal_volume
43pub_type
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