Abstract:
:As an improved strategy for producing functional macromolecules by in vitro evolutionary optimization, we propose an automated machine that can process up to 960 samples in parallel. It consists of a 960-well PCR machine with special sealed plastic reaction vessels and appropriate handling devices. We show that the heat-sealing technique does not significantly affect the activity of Taq DNA Polymerase or that of the temperature-sensitive Q beta RNA polymerase but avoids cross-contamination and evaporation of the samples. Initial experiments demonstrate the suitability of the apparatus to uniformly process the samples and to perform the thermocycling. Serial transfer of reaction products into fresh reaction solution was used to initiate further rounds of amplification as a typical experimental setup for an evolutionary biotechnology application.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Schober A,Walter NG,Tangen U,Strunk G,Ederhof T,Dapprich J,Eigen Msubject
Has Abstractpub_date
1995-04-01 00:00:00pages
652-61issue
4eissn
0736-6205issn
1940-9818journal_volume
18pub_type
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