Abstract:
:A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when expressed in mammalian cells. The utility of EGFP-puro as a selectable/screenable marker was demonstrated by the ease with which a recombinant guinea pig cytomegalovirus containing EGFP-puro was isolated by a combination of puromycin selection and screening for green fluorescence. We conclude that EGFP-puro is a compact and versatile marker that should prove useful for recombinant virus and transgenic cell line construction, particularly in applications in which coding capacity is limited.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Abbate J,Lacayo JC,Prichard M,Pari G,McVoy MAdoi
10.2144/01312st05subject
Has Abstractpub_date
2001-08-01 00:00:00pages
336-40issue
2eissn
0736-6205issn
1940-9818journal_volume
31pub_type
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