Abstract：
:Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Hegde P,Qi R,Abernathy K,Gay C,Dharap S,Gaspard R,Hughes JE,Snesrud E,Lee N,Quackenbush Jdoi
10.2144/00293bi01subject
Has Abstractpub_date
2000-09-01 00:00:00pages
548-50, 552-4, 556 passimissue
3eissn
0736-6205issn
1940-9818journal_volume
29pub_type
杂志文章,评审相关文献
BIOTECHNIQUES文献大全abstract：:Few studies have focused on the significance of ras protein levels in human malignancy, in part because of the inherent difficulty in quantitation of the ras gene product. We have developed a method for the enzymatic determination of the ras gene product and have used this method for the quantitation of ras gene produ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and e...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:Planar lipid bilayers represent a versatile platform for studying the functions of various membrane proteins as well as the development of biosensors. Despite the continuing technological progress in the fabrication of low-noise bilayer setups with mechanically and electrically stable planar bilayers, there is still a...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000112384
更新日期：2007-03-01 00:00:00
abstract：:Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene synthesis or decrease variability when applied to the constructi...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000114330
更新日期：2015-09-01 00:00:00
abstract：:The trend toward high-throughput techniques in molecular biology and the explosion of online scientific data threaten to overwhelm the ability of researchers to take full advantage of available information. This problem is particularly severe in the rapidly expanding area of gene expression experiments, for example, t...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/99276bc03
更新日期：1999-12-01 00:00:00
abstract：:We describe here a simple and rapid method for enzymatic DNA amplification using DNA template recovered from membrane filters previously used in hybridization analysis. This is done by first solubilizing membrane pieces carrying DNA of interest in dimethyl sulfoxide, followed by isopropanol precipitation and polymeras...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:A simple spectrophotometric method for measuring DNA in proliferating cells is described. The method is an adaptation of the widely used diphenylamine (DPA) reaction to examine DNA in cells grown in a 96-well plate. This assay was capable of detecting as little as 10 ng DNA and could be used to measure DNA in stored a...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
abstract：:Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations...
journal_title：BioTechniques
pub_type： 信件
doi：10.2144/btn-2020-0012
更新日期：2020-06-01 00:00:00
abstract：:We describe a simple and cost-effective method for the synthesis of an internal fluorescently labeled DNA standard for fragment sizing using an automated DNA sequencer. A set of primer pairs labeled with ROX was developed to amplify 12 DNA fragments, 58-417 bp, derived from a conserved region of plant chloroplast DNA....
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/01314st06
更新日期：2001-10-01 00:00:00
abstract：:The relative spatial distribution of cells in a solid tumor contributes to development of malignancy, yet the details of this process remain poorly understood. To elucidate these mechanisms, the ability to extract and analyze the entire DNA content of individual cells whose precise location in the tumor is known is re...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000113860
更新日期：2012-04-01 00:00:00
abstract：:Rapid cycle DNA amplification was continuously monitored by three different fluorescence techniques. Fluorescence was monitored by (i) the double-strand-specific dye SYBR Green I, (ii) a decrease in fluorescein quenching by rhodamine after exonuclease cleavage of a dual-labeled hydrolysis probe and (iii) resonance ene...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/97221bi01
更新日期：1997-01-01 00:00:00
abstract：:Neuronal death can be induced by DNA-damaging agents and occurs by apoptosis involving a specific signal-transduction pathway. However, to our knowledge, methods for the quantitative determination of DNA damage in individual neurons have not yet been described. Here we optimize the single-cell gel electrophoresis (SCG...
journal_title：BioTechniques
pub_type：
doi：10.2144/99262st02
更新日期：1999-02-01 00:00:00
abstract：:The increasing interest in manipulating neural circuits in developing brains has created a demand for reliable and accurate methods for delivering viruses to newborn mice. Here we describe a novel 3D-printed mouse neonatal stereotaxic adaptor for intracerebral viral injection that provides enhanced precision and relia...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/btn-2020-0050
更新日期：2020-10-01 00:00:00
abstract：:We have developed an automated purification method for dye-terminator-based DNA sequencing products using a magnetic bead approach. This 384-well protocol generates sequence fragments that are essentially free of template DNA, salt, and excess dye-terminator products. In comparison with traditional ethanol precipitati...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/02326st05
更新日期：2002-06-01 00:00:00
abstract：:Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material. We describe a new technique, polymerase chain displacement reaction (PCDR), which uses multiple nested primers in...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000113951
更新日期：2013-02-01 00:00:00
abstract：:A labeled peptide nucleic acid (PNA) antisense probe was used to study the spatial distribution of triplet repeats (CTG) in human myotonic dystrophy (DM) cells by high-resolution fluorescence in situ hybridization (FISH). It was found that transcripts containing triplet repeats were present as a number of discrete foc...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/98243rr02
更新日期：1998-03-01 00:00:00
abstract：:SOP3 is a web-based software tool for designing oligonucleotide primers for use in the analysis of single nucleotide polymorphisms (SNPs). Accessible via the Internet, the application is optimized for developing the PCR and sequencing primers that are necessary for Pyrosequencing. The application accepts as input gene...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/05381RR01
更新日期：2005-01-01 00:00:00
abstract：:The use of AlphaScreen® detection has allowed researchers to examine a wide variety of molecular interactions for use in high-throughput screening. However, the cost of Alpha reagents can often be prohibitory for extended screening campaigns or for young investigators with limited funding. To reduce assay costs, many ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/btn-2018-2001
更新日期：2018-04-01 00:00:00
abstract：:Virus uptake is the first rate-limiting step for the successful gene delivery of any virus-based gene therapy. For adenovirus-based gene therapy, the expression levels of the adenovirus receptor--coxsackievirus and adenovirus receptor (CAR)--play an important role in dictating gene delivery. We have observed a wide sp...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/03351dd02
更新日期：2003-07-01 00:00:00
abstract：:Pairs of primers flanking known miniTn10 transposon insertion sites were used to confirm the presence of the transposon in DNA isolated from Legionella pneumophila mutants. It was expected that the polymerase chain reaction products derived from the mutant template would be larger than those from the wild-type (WT) te...
journal_title：BioTechniques
pub_type：
doi：10.2144/99273st04
更新日期：1999-09-01 00:00:00
abstract：:We have conducted a systematic evaluation of the calculated molecular properties of compounds in clinical development and have found that the development process selects for compounds that have certain computed physical properties. In particular, as the stage of development progresses, compounds that are advanced have...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：2003-06-01 00:00:00
abstract：:The PCR method has proved to be an invaluable tool for the specific and sensitive detection of genetically modified material (e.g., Roundup Ready Soybean and Bt-176 "Maximizer" Maize) in foodstuffs. The first step in the procedure, namely the purification of nucleic acids from the sample, is often the deciding factor ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/01312pf01
更新日期：2001-08-01 00:00:00
abstract：:Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to solve two design problems: combining all markers into the smalle...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000113156
更新日期：2009-06-01 00:00:00
abstract：:The possibility to miniaturize and parallelize biological assays has a great impact on the development of biomedical technologies. Here, we describe a simple, miniaturized, and parallelized method employing entire cells from different cell lines displaying a protein of interest on their surface, which were immobilized...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：2002-12-01 00:00:00
abstract：:Using a simple electronic circuit, a thermocouple can be connected to a chart recorder to measure the actual temperature inside a PCR tube. This allows accurate inspection of the thermocycle program and comparison between thermoprofiles from different thermocyclers. We found that the recording of temperature cycling e...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation. ...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1991-01-01 00:00:00
abstract：:We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes. ...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:Francisella tularensis is one of the most deadly bacterial agents, yet most of the genetic determinants of pathogenesis are still unknown. We have developed an efficient targeted mutagenesis strategy in the model organism F. tularensis subsp. novicida by utilizing universal priming of optimized antibiotic resistance c...
journal_title：BioTechniques
pub_type： 杂志文章
doi：10.2144/000112574
更新日期：2007-10-01 00:00:00
abstract：:Procedures utilizing Chelex 100 chelating resin have been developed for extracting DNA from forensic-type samples for use with the PCR. The procedures are simple, rapid, involve no organic solvents and do not require multiple tube transfers for most types of samples. The extraction of DNA from semen and very small blo...
journal_title：BioTechniques
pub_type： 杂志文章
doi：
更新日期：1991-04-01 00:00:00
abstract：:Data are presented illustrating the optimum concentration range of reverse transcription PCR-generated products under 500 bp for accurate base calling with direct automated DNA sequencing. A 357-bp fragment of the human estrogen receptor, which includes the DNA binding domain of the protein, was used as a representati...
journal_title：BioTechniques
pub_type：
doi：
更新日期：1994-09-01 00:00:00
