Identification of anti-invasive but noncytotoxic chemotherapeutic agents using the tetrazolium dye MTT to quantitate viable cells in Matrigel.


:Screening methods for chemotherapeutic agents usually rely on the cytotoxic properties of the drugs. However, agents that inhibit invasion may have more efficacy and cause fewer side effects. Various cellular invasion assays have been used to evaluate these types of compounds, including the modified Boyden chamber, monolayer wound models and Matrigel outgrowth assays. In this report, we have combined the use of the Matrigel outgrowth assay with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) visualization and cell viability dye to visualize invasive cells on Matrigel without magnification. Extraction of the dye's formazan byproduct allows cell viability to be assessed. Using several invasive and noninvasive cell lines, the utility of the method for various target cells was verified. Several established chemotherapeutic agents were also screened for their anti-invasive and/or cytotoxic effects when cultured on Matrigel. Our results suggest that this method may be an easy, inexpensive and nonradioactive alternative for both enumerating cells on Matrigel and screening various tumor cell lines treated with chemotherapeutic agent to look for compounds with noncytotoxic but anti-invasive properties.






Sasaki CY,Passaniti A




Has Abstract


1998-06-01 00:00:00












  • Measurement of prostaglandin G/H synthase and lipoxygenase activity in the stomach wall by HPLC.

    abstract::A method for the determination of prostaglandin G/H synthase and lipoxygenase activities in tissues was developed and employed with rat gastric mucosa samples. Tissues and microsomes were incubated in a buffer containing nonionic detergent and 1.32 mM arachidonic acid for 10 min. Following extraction with ethyl acetat...


    pub_type: 杂志文章


    authors: Stein TA,Bailey B,Auguste LJ,Wise L

    更新日期:1991-02-01 00:00:00

  • Absolute quantitation of cancer-related proteins using an MS-based peptide chip.

    abstract::New technologies are needed that can diagnose cancer more rapidly and accurately. These technologies must also have the ability to identify the particular cellular abnormalities contributing to the malignancy, thus directing the appropriate treatments. Such technologies should permit absolute quantitation of specific ...


    pub_type: 杂志文章


    authors: Warren EN,Jiang J,Parker CE,Borchers CH

    更新日期:2005-06-01 00:00:00

  • High-quality RNA and DNA from flow cytometrically sorted human epithelial cells and tissues.

    abstract::Microarray technologies have made possible comprehensive analyses of nucleic acid sequence and expression. However, the technology to obtain efficiently high-quality RNA and DNA suitable for array analysis from purified populations of neoplastic cells from human tissues has not been well addressed. Microdissection can...


    pub_type: 杂志文章


    authors: Barrett MT,Glogovac J,Prevo LJ,Reid BJ,Porter P,Rabinovitch PS

    更新日期:2002-04-01 00:00:00

  • Eukaryotic GST fusion vector for the study of protein-protein associations in vivo: application to interaction of ATFa with Jun and Fos.

    abstract::We describe a multipurpose eukaryotic expression vector that incorporates the following features: restriction sites for in-frame insertion of cDNAs of interest between sequences encoding the glutathione-S-transferase (GST) and an oligohistidine element, allowing expression of the corresponding fusion proteins; a phosp...


    pub_type: 杂志文章


    authors: Chatton B,Bahr A,Acker J,Kedinger C

    更新日期:1995-01-01 00:00:00

  • Innovative proteomic approaches for cancer biomarker discovery.

    abstract::Substantial technological advances in proteomics and related computational science have been made in the past few years. These advances overcome in part the complexity and heterogeneity of the human proteome, permitting quantitative analysis and identification of protein changes associated with tumor development. Here...


    pub_type: 杂志文章,评审


    authors: Faca V,Krasnoselsky A,Hanash S

    更新日期:2007-09-01 00:00:00

  • An electrophoretic capture method for efficient recovery of rare sequences from heterogeneous DNA.

    abstract::It is difficult to isolate rare, PCR-quality DNA from specimens containing large quantities of nonspecific DNA from multiple sources (heterogeneous DNA). Extracting human DNA from stool for colorectal cancer (CRC) screening tests exemplifies this technically challenging sample preparation problem. The stool matrix is ...


    pub_type: 杂志文章


    authors: Kent Moore J,Smith JA,Whitney DH,Durkee KH,Shuber AP

    更新日期:2008-03-01 00:00:00

  • A DNA cassette containing a trimerized SV40 polyadenylation signal which efficiently blocks spurious plasmid-initiated transcription.

    abstract::A head-to-tail trimer of the SV40 Bcl I-Bam H1 DNA fragment, specifying polyadenylation of RNA transcripts, was cloned as a cassette flanked by multiple restriction sites. Insertion of the trimer into several expression vectors efficiently prevented spurious expression of reporter genes resulting from transcriptional ...


    pub_type: 杂志文章


    authors: Maxwell IH,Harrison GS,Wood WM,Maxwell F

    更新日期:1989-03-01 00:00:00

  • High-sensitivity hybridization assay for quantitation of residual E. coli DNA.

    abstract::Impurity assays for recombinant protein therapeutics are essential to ensure batch-to-batch consistency and to meet the FDA's criteria for a well-characterized biopharmaceutical. For determination of residual host cell DNA, membrane hybridization assays utilizing radiolabeled DNA probes prepared from the host cell's g...




    authors: Ji X,Lee K,DiPaolo B

    更新日期:2002-05-01 00:00:00

  • Human multiple tissue western blots: a new immunological tool for the analysis of tissue-specific protein expression.

    abstract::Human multiple tissue Western (MTW) blots are premade immunoblots prepared using proteins isolated from adult human tissue. The proteins are isolated from whole tissue homogenates under conditions designed to minimize proteolysis and to ensure maximal representation of tissue-specific proteins. Sodium dodecyl sulfate ...


    pub_type: 杂志文章


    authors: Kain SR,Mai K,Sinai P

    更新日期:1994-11-01 00:00:00

  • Simplified DGS procedure for large-scale genome structural study.

    abstract::Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the ...


    pub_type: 杂志文章


    authors: Jung YC,Xu J,Chen J,Kim Y,Winchester D,Wang SM

    更新日期:2009-11-01 00:00:00

  • Selection of scFv phages on intact cells under low pH conditions leads to a significant loss of insert-free phages.

    abstract::Display of functional antibody fragments on the surface of filamentous bacteriophages allows fast selection of specific phage antibodies against a variety of target antigens. However, enrichment of single chain variable fragment (scFv)-displaying phages is often hampered by the abundance of bacteriophages lacking anti...


    pub_type: 杂志文章


    authors: Tur MK,Huhn M,Sasse S,Engert A,Barth S

    更新日期:2001-02-01 00:00:00

  • CRISPR vs COVID-19: how can gene editing help beat a virus?

    abstract::[Formula: see text] Known to be a sturdy weapon in a scientist's arsenal, how has the gene editing tool CRISPR been applied in the fight against COVID-19? ...


    pub_type: 新闻


    authors: Straiton J

    更新日期:2020-11-01 00:00:00

  • High-throughput cDNA screening utilizing a low order neural network filter.

    abstract::A low order neural network-based filter was designed as a rapid screening agent for single-spanning transmembrane regions in an integrated informatics system. A rapid screening algorithm was seen as a compromise between costly structure-specific techniques and simple rules that gave a high false-positive rate for cDNA...


    pub_type: 杂志文章


    authors: Huang GM,Farkas J,Hood L

    更新日期:1996-12-01 00:00:00

  • Improved sensitivity for solid-support invasive cleavage reactions with flow cytometry analysis.

    abstract::A new configuration of the solid-support invasive cleavage reaction provides a small reaction-volume format for high-sensitivity discrimination of nucleic acid targets with single nucleotide differences. With target concentrations as low as 2 amol/assay, the solid-support invasive cleavage reaction clearly distinguish...




    authors: Stevens PW,Rao KV,Hall JG,Lyamichev V,Neri BP,Kelso DM

    更新日期:2003-01-01 00:00:00

  • Pilot-scale production of murine monoclonal antibodies in agitated, ceramic-matrix or hollow-fiber cell culture systems.

    abstract::The purpose of this research was to compare three bioreactor systems for the pilot-scale production of monoclonal antibodies (MAbs). We needed to produce gram quantities of murine MAbs against human prostatic acid phosphatase for use in fragmentation, radiolabeling and in vivo radio-imaging studies. The stable hybrido...


    pub_type: 杂志文章


    authors: Kurkela R,Fraune E,Vihko P

    更新日期:1993-10-01 00:00:00

  • Delivery of protein into cells using polycationic liposomes.

    abstract::We describe a procedure for delivery of purified proteins into a variety of tissue culture cells using a new polycationic lipid preparation, LipofectAMINE. Several different proteins, with diverse physical properties, can be delivered into cells by this method. Compared with commercially available monocationic lipids,...




    authors: Sells MA,Li J,Chernoff J

    更新日期:1995-07-01 00:00:00

  • Gene and library synthesis without amplification: polymerase step reaction (PSR).

    abstract::Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene synthesis or decrease variability when applied to the constructi...


    pub_type: 杂志文章


    authors: Lee ZB,Firnhaber C,Clarke J,DeDecker BS

    更新日期:2015-09-01 00:00:00

  • High-throughput generation of sequence indexes from T-DNA mutagenized Arabidopsis thaliana lines.

    abstract::A pipeline has been created for the characterization of Arabidopsis thaliana mutants by generating flanking sequence tags (FSTs) and optimized for economic, high-throughput production. The GABI-Kat collection of T-DNA mutagenized A. thaliana plants was used as a source of independent transgenic lines. The pipeline inc...


    pub_type: 杂志文章


    authors: Strizhov N,Li Y,Rosso MG,Viehoever P,Dekker KA,Weisshaar B

    更新日期:2003-12-01 00:00:00

  • Genetic immunization for antibody generation in research animals by intravenous delivery of plasmid DNA.

    abstract::Genetic immunization is an attractive approach to generate antibodies because native proteins are expressed in vivo with normal posttranscriptional modifications, avoiding time-consuming and costly antigen isolation or synthesis. Hydrodynamic tail or limb vein delivery of naked plasmid DNA expression vectors was used ...


    pub_type: 杂志文章


    authors: Bates MK,Zhang G,Sebestyén MG,Neal ZC,Wolff JA,Herweijer H

    更新日期:2006-02-01 00:00:00

  • Microsatellite marker discovery using single molecule real-time circular consensus sequencing on the Pacific Biosciences RS.

    abstract::Microsatellite sequences are important markers for population genetics studies. In the past, the development of adequate microsatellite primers has been cumbersome. However with the advent of next-generation sequencing technologies, marker identification in genomes of non-model species has been greatly simplified. Her...


    pub_type: 杂志文章


    authors: Grohme MA,Soler RF,Wink M,Frohme M

    更新日期:2013-11-01 00:00:00

  • Fluorescent in situ hybridization of maize meiotic chromosomes as visualized by confocal microscopy.

    abstract::Knob heterochromatin served as the model for the development of fluorescent chromosome in situ hybridization on maize meiotic chromosomes. The meiotic chromosomes were hybridized with a digoxigenin-labeled RNA probe of the knob repeat sequence that is a component of the morphologically determined knob heterochromatin....




    authors: Makowski ER,Ruzin SE

    更新日期:1994-02-01 00:00:00

  • Cautionary note on the use of Caenorhabditis elegans to study muscle phenotypes caused by mutations in the human MYH7 gene.

    abstract::Mutations in the human MYH7 gene, encoding a slow skeletal muscle/β-cardiac myosin heavy chain, cause different types of myopathies. The nematode model Caenorhabditis elegans has frequently been employed to study the molecular and physiological consequences of MYH7 mutations in muscle function by introducing mutations...


    pub_type: 信件


    authors: Gil-Gálvez A,Carbonell-Corvillo P,Paradas C,Miranda-Vizuete A

    更新日期:2020-06-01 00:00:00

  • Use of CMFDA and CMTMR fluorescent dyes in FACS-based antibody screening.

    abstract::Cell-based immunizations are often used when membrane antigens are difficult to purify. To confirm that an antibody binding to the surface of a cell line is, in fact, binding to the desired antigen, FACS can be performed independently on two cell lines, a transfected cell line expressing the antigen of interest and a ...




    authors: Yang XP,Gallo M,Ngan I,Nocerini M,Chen MM

    更新日期:2002-03-01 00:00:00

  • Use of Multiscreen plates for the preparation of bacterial DNA suitable for PCR.

    abstract::A rapid and inexpensive method for isolating bacterial DNA for use in PCR is described. The method is based on the guanidinium thiocyanate (GuSCN)-lysis method of Boom et al. (J. Clin. Microbiol. 28:495-503, 1990) and enables a multiple of 96 samples to be prepared in only one hour. We use Multiscreen plates and a vac...


    pub_type: 杂志文章


    authors: Reek FH,Smits MA,Kamp EM,Smith HE

    更新日期:1995-08-01 00:00:00

  • Rapid regeneration of virus from cells infected with a retroviral vector.

    abstract::Recombinant retroviral vectors usually encode the genes of interest in place of the viral structural genes, which must be provided in trans. These viruses are therefore defective for replication: infected cells cannot produce progeny virus. However, in some cases it may be desirable to generate virus from an infected ...


    pub_type: 杂志文章


    authors: Corbley MJ,Cherington V,Feig LA,Cooper GM,Roberts TM

    更新日期:1994-12-01 00:00:00

  • Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II.

    abstract::A simple, non-destructive procedure is described to determine the quality of DNA arrays before they are used. It consists of a preliminary staining step of the DNA microarray by using SYBR green II, a fluorophore with specific affinity for ssDNA, followed by a laser scan analysis. The surface quality, integrity and ho...




    authors: Battaglia C,Salani G,Consolandi C,Bernardi LR,De Bellis G

    更新日期:2000-07-01 00:00:00

  • Telomerase assay using biotinylated-primer extension and magnetic separation of the products.

    abstract::Human telomerase, a ribonucleoprotein enzyme is known to be associated with immortalized cancer cells but is absent in most normal tissues. Thus, telomerase appears to be an attractive new target for anticancer agents and an important diagnostic marker of human cancers. Here, we describe an improved telomerase assay m...


    pub_type: 杂志文章


    authors: Sun D,Hurley LH,Von Hoff DD

    更新日期:1998-12-01 00:00:00

  • A rapid and simple method for labeling short DNA fragments using Taq polymerase.

    abstract::This report describes a new method for labeling PCR-generated short length (60-120 bp) double-stranded DNA fragments for use as hybridization probes. The method utilizes gene-specific primers identical to those for PCR generation of non-radioactive DNA fragments. Radioactive probes are synthesized by Taq DNA polymeras...


    pub_type: 杂志文章


    authors: Mizobuchi M,Frohman LA

    更新日期:1992-03-01 00:00:00

  • A new approach to follow a single extracellular vesicle-cell interaction using optical tweezers.

    abstract::Extracellular vesicles (EVs) are spherical membrane structures released by most cells. These highly conserved mediators of intercellular communication carry proteins, lipids, and nucleic acids, and transfer these cellular components between cells by different mechanisms, such as endocytosis, macropinocytosis, or fusio...


    pub_type: 杂志文章


    authors: Prada I,Amin L,Furlan R,Legname G,Verderio C,Cojoc D

    更新日期:2016-01-01 00:00:00

  • PCR-introduced loop structure as primer in DNA sequencing.

    abstract::The need for a primer hybridization step before sequencing has been eliminated using a stem-loop structure generated by PCR. The loop structure is obtained by careful design of the PCR primer or by cloning the target DNA into a dedicated vector (pRIT 28HP). After solid-phase capture of the PCR product, the loop is for...


    pub_type: 杂志文章


    authors: Ronaghi M,Pettersson B,Uhlén M,Nyrén P

    更新日期:1998-11-01 00:00:00