Sequencing reactions in microtiter plates.

Abstract:

:We describe a simple and inexpensive method of performing sequencing reactions for 24 single-strand M13 DNA clones in microtiter plates. To simplify elevated temperature incubations during sequencing reactions, two heating blocks were designed to accommodate microtiter plates and fit within common laboratory heating modules. With only slight modification of standard fluorescent and radioisotopic sequencing methods, the sequencing reactions for 24 clones can be done in as little as 40 minutes.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Koop BF,Wilson RK,Chen C,Halloran N,Sciammis R,Hood L,Lindelien JW

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

32, 34-7

issue

1

eissn

0736-6205

issn

1940-9818

journal_volume

9

pub_type

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