Abstract:
:The isolation of a single DNA molecule for cloning is technically difficult, and the subsequent screening of colonies for recombinant DNA clones is time consuming. Ion pair-reversed phase HPLC (IP-RP-HPLC) analysis of nucleic acids improves the resolution and isolation of PCR products to be cloned. We demonstrate that PCR products analyzed and collected using the IP-RP-HPLC approach (WAVE DNA Fragment Analysis System) can be cloned directly into a plasmid vector. In addition, we demonstrate that when IP-RP-HPLC analysis is extended to the colony screening process, the time required for these procedures is reduced.
journal_name
Biotechniquesjournal_title
BioTechniquesauthors
Shaw-Bruha CM,Lamb KAdoi
10.2144/00284pf02subject
Has Abstractpub_date
2000-04-01 00:00:00pages
794-7issue
4eissn
0736-6205issn
1940-9818journal_volume
28pub_type
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