Patents and patent office resources in biotechnology.

Abstract:

:Patents play an increasingly important role in the dissemination of information in many fast moving fields such as biotechnology and semiconductors. Quite a few new developments are introduced as patents, and only later, if at all, do they find their way into the scientific literature. In spite of this, patents lack wide acceptance as a source of information among scientists in academia and, to a lesser degree, industry. Patents share many similarities with scientific papers. They both are organized in a similar way and are carefully reviewed by experts in the field. Both can be effective and timely sources of information. Patents can be accessed through data bases, library collections, the "Official Gazette of the Patent and Trademark Office," or directly in the Patent and Trademark Office. This article is designed to serve as a guide to the type of information which can be found in patents, and alternatives for obtaining this information.

journal_name

Biotechniques

journal_title

BioTechniques

authors

Koniarek JP,Coleman KD

subject

Has Abstract

pub_date

1988-02-01 00:00:00

pages

148-53

issue

2

eissn

0736-6205

issn

1940-9818

journal_volume

6

pub_type

杂志文章
  • Self-seal reagent: evaporation control for molecular histology procedures without chambers, clips or fingernail polish.

    abstract::Sensitive nucleic acid based detection methods such as in situ PCR, in situ RT-PCR and PRINS have great potential in the areas of developmental biology, pathogenesis and diagnostics. However, control of evaporation from in situ reactions is critical to ensure reliable data. Self-Seal Reagent, a component added directl...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/97232pf01

    authors: Sullivan DE,Bobroski LE,Bagasra O,Finney M

    更新日期:1997-08-01 00:00:00

  • High-sensitivity hybridization assay for quantitation of residual E. coli DNA.

    abstract::Impurity assays for recombinant protein therapeutics are essential to ensure batch-to-batch consistency and to meet the FDA's criteria for a well-characterized biopharmaceutical. For determination of residual host cell DNA, membrane hybridization assays utilizing radiolabeled DNA probes prepared from the host cell's g...

    journal_title:BioTechniques

    pub_type:

    doi:10.2144/02325dd06

    authors: Ji X,Lee K,DiPaolo B

    更新日期:2002-05-01 00:00:00

  • Method for isolation of PCR-ready genomic DNA from zebrafish tissues.

    abstract::Here we describe a method for the isolation of PCR-ready genomic DNA from various zebrafish tissues that is based on a previously published murine protocol. The DNA solutions are of sufficient quality to allow PCR detection of transgenes from all commonly used zebrafish tissues. In sperm, transgene amplification was s...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000112619

    authors: Meeker ND,Hutchinson SA,Ho L,Trede NS

    更新日期:2007-11-01 00:00:00

  • Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR.

    abstract::We describe an expeditious method for the isolation of cDNA clones utilizing PCR-based amplification of target sequences from cDNA libraries. This method is rapid, less labor-intensive and inexpensive when compared with screening libraries with radiolabeled probes. This method can be applied to isolate multiple member...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Isola NR,Harn HJ,Cooper DL

    更新日期:1991-11-01 00:00:00

  • A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria.

    abstract::A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this wor...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/btn-2018-0074

    authors: Texier P,Coddeville M,Bordes P,Genevaux P

    更新日期:2018-09-01 00:00:00

  • Limiting detection of an amplification signal for HLA-D region and VNTR genes by 32P-PCR.

    abstract::The limiting detection signal for identification of human genetic markers, such as HLA-D and VNTR genes, was determined using DNA isolated from a series of decreasing numbers of lymphocytes carrying the target marker in the polymerase chain reaction (PCR). The PCR procedure was assembled by incorporating 32P-labeled d...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: McDaniel DO,Naftilan J,Barber WH

    更新日期:1993-07-01 00:00:00

  • Specific suppression of false-positive signals in the product-enhanced reverse transcriptase assay.

    abstract::The recently developed ultrasensitive reverse transcriptase (RT) test involving a PCR step can detect minute amounts of RT in single retroviral particles and is 10(6) times more sensitive than conventional RT assays. We have found that different DNA-dependent DNA polymerases like DNA Polymerase I from Escherichia coli...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Lugert R,König H,Kurth R,Tönjes RR

    更新日期:1996-02-01 00:00:00

  • [Letter to the Editor] Many commercial hot-start polymerases demonstrate activity prior to thermal activation.

    abstract::Address correspondence to Martin A. Kennedy, Department of Pathology & Carney Centre for Pharmacogenomics, University of Otago, Christchurch, PO Box 4345, Christchurch, New Zealand. E-mail: martin.kennedy@otago.ac.nz. ...

    journal_title:BioTechniques

    pub_type: 信件

    doi:10.2144/000114481

    authors: Stevens AJ,Appleby S,Kennedy MA

    更新日期:2016-12-01 00:00:00

  • Improvements to bead-based oligonucleotide ligation SNP genotyping assays.

    abstract::We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000112960

    authors: Bruse S,Moreau M,Azaro M,Zimmerman R,Brzustowicz L

    更新日期:2008-11-01 00:00:00

  • New primer strategy improves precision of differential display.

    abstract::To increase the reproducibility and to reduce the false positives in the initial mRNA differential display, modified long composite primers were developed based on both mRNA differential display and RNA arbitrarily primed PCR fingerprinting methods. Ten-base nucleotides were added at the 5' ends of the primers used in...

    journal_title:BioTechniques

    pub_type:

    doi:

    authors: Zhao S,Ooi SL,Pardee AB

    更新日期:1995-05-01 00:00:00

  • PART I: FIGHTING CANCER WITH DEADLY BACTERIA.

    abstract::A 200-year-old observation might provide a new way to eliminate tumors by infecting cancer patients with bacteria. Kristie Nybo explores a new approach that could transform cancer treatment. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/000114624

    authors: Nybo K

    更新日期:2018-01-01 00:00:00

  • Human multiple tissue western blots: a new immunological tool for the analysis of tissue-specific protein expression.

    abstract::Human multiple tissue Western (MTW) blots are premade immunoblots prepared using proteins isolated from adult human tissue. The proteins are isolated from whole tissue homogenates under conditions designed to minimize proteolysis and to ensure maximal representation of tissue-specific proteins. Sodium dodecyl sulfate ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Kain SR,Mai K,Sinai P

    更新日期:1994-11-01 00:00:00

  • The era of 'omics unlimited.

    abstract::Determining the primary sequences of informational macromolecules is no longer a limiting factor for our ability to completely understand the biological functioning of cells and organisms. Similarly, our understanding of transcriptional regulation (transcriptomics) has been greatly enhanced by the availability of micr...

    journal_title:BioTechniques

    pub_type: 杂志文章,评审

    doi:10.2144/000113137

    authors: Kandpal R,Saviola B,Felton J

    更新日期:2009-04-01 00:00:00

  • Automated system for capture and detection of nucleic acids.

    abstract::A fully automated nucleic acid analysis system is described, which offers positive sample identification, improved sensitivity and reduced user interaction compared to conventional techniques. The system relies on the sequence-specific capture of DNA onto solid-phase particles, confirming product identity without the ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/99271pf01

    authors: Brown A,Akinsanya AA,Barker SJ,Brophy M,Dobb AK,Doyle SM,Hudson IR,Minter SJ,Wraith MJ,Oultram JD

    更新日期:1999-07-01 00:00:00

  • DIRECTING DRUG EVOLUTION.

    abstract::Directed evolution is poised to change small molecule discovery and provide greater access to "drug space." Sarah Webb looks into the evolving drug discovery landscape. ...

    journal_title:BioTechniques

    pub_type: 新闻

    doi:10.2144/000114616

    authors: Webb S Ph D

    更新日期:2017-12-01 00:00:00

  • PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs.

    abstract::The PCR method has proved to be an invaluable tool for the specific and sensitive detection of genetically modified material (e.g., Roundup Ready Soybean and Bt-176 "Maximizer" Maize) in foodstuffs. The first step in the procedure, namely the purification of nucleic acids from the sample, is often the deciding factor ...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01312pf01

    authors: Tengel C,Schüssler P,Setzke E,Balles J,Sprenger-Haussels M

    更新日期:2001-08-01 00:00:00

  • Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.

    abstract::A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and e...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Brenner CA,Tam AW,Nelson PA,Engleman EG,Suzuki N,Fry KE,Larrick JW

    更新日期:1989-11-01 00:00:00

  • Adenovirus-mediated transfection of cultured cells.

    abstract::We describe here a simple and efficient transfection method for transient expression of cloned genes in cell lines and primary cultured cells. The method involves the use of DEAE-dextran to target DNA to the cellular endocytotic pathway and the use of a human adenovirus to ensure efficient lysis of endosomal vesicles....

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Forsayeth JR,Garcia PD

    更新日期:1994-08-01 00:00:00

  • Glycerol enhancement of ligand-polylysine/DNA transfection.

    abstract::Primary human fibroblasts and a series of cell lines (A549, BNL CL.2, H225, NIH 3T3 and Rat-1) are efficiently transfected by using positively charged complexes of plasmid DNA and transferrin-polylysine or polylysine in the presence of glycerol (1 molar to 1.8 molar, depending on the cell type). An increase in gene ex...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/96205rr04

    authors: Zauner W,Kichler A,Schmidt W,Sinski A,Wagner E

    更新日期:1996-05-01 00:00:00

  • Surveying the repair of ancient DNA from bones via high-throughput sequencing.

    abstract::DNA damage in the form of abasic sites, chemically altered nucleotides, and strand fragmentation is the foremost limitation in obtaining genetic information from many ancient samples. Upon cell death, DNA continues to endure various chemical attacks such as hydrolysis and oxidation, but repair pathways found in vivo n...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/000114307

    authors: Mouttham N,Klunk J,Kuch M,Fourney R,Poinar H

    更新日期:2015-07-01 00:00:00

  • Generation and characterization of recombinant vascular targeting agents from hybridoma cell lines.

    abstract::Vascular targeting agents (VTAs) can be produced by linking antibodies or antibody fragments directed against endothelial cell markers to effector moieties. So far, it has been necessary to produce the components of VTAs (antibody, antibody fragment, linker, and effector) separately and, subsequently, to conjugate the...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/01301dd03

    authors: Gottstein C,Wels W,Ober B,Thorpe PE

    更新日期:2001-01-01 00:00:00

  • Examination of the computed molecular properties of compounds selected for clinical development.

    abstract::We have conducted a systematic evaluation of the calculated molecular properties of compounds in clinical development and have found that the development process selects for compounds that have certain computed physical properties. In particular, as the stage of development progresses, compounds that are advanced have...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Blake JF

    更新日期:2003-06-01 00:00:00

  • Preparation and characterization of immunoliposomes for targeting of antiviral agents.

    abstract::Antibodies specific to avian myeloblastosis virus envelope glycoprotein gp80 were raised. Immunoliposomes were prepared using anti-avian myeloblastosis virus envelope glycoprotein gp80 antibody. The antibody was palmitoylated to facilitate its incorporation into lipid bilayers of liposomes. The fluorescence emission s...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Kalvakolanu DV,Abraham A

    更新日期:1991-08-01 00:00:00

  • High-throughput generation of sequence indexes from T-DNA mutagenized Arabidopsis thaliana lines.

    abstract::A pipeline has been created for the characterization of Arabidopsis thaliana mutants by generating flanking sequence tags (FSTs) and optimized for economic, high-throughput production. The GABI-Kat collection of T-DNA mutagenized A. thaliana plants was used as a source of independent transgenic lines. The pipeline inc...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/03356st01

    authors: Strizhov N,Li Y,Rosso MG,Viehoever P,Dekker KA,Weisshaar B

    更新日期:2003-12-01 00:00:00

  • Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR.

    abstract::Real-time reverse transcription PCR (RT-PCR) is a sensitive and accurate method to monitor gene expression and is often used to profile the expression of putative tumor antigens in the context of immunotherapy. However, this technique consists of several steps, including cell processing, RNA extraction, RNA storage, a...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/04361ST04

    authors: Aerts JL,Gonzales MI,Topalian SL

    更新日期:2004-01-01 00:00:00

  • Paradoxical effect of eukaryotic expression vectors on reporters.

    abstract::A critical issue in transfection or co-transfection experiments is to define the appropriate controls. In most cases, a corresponding empty vector is used as one control. We report a paradoxical effect of empty mammalian expression vectors on different reporters. We have found that different empty vectors can inhibit ...

    journal_title:BioTechniques

    pub_type:

    doi:10.2144/02331st04

    authors: Hu Q,Suzuki K,Hirschler-Laszkiewicz I,Rothblum LI

    更新日期:2002-07-01 00:00:00

  • Fluorescent in situ hybridization of maize meiotic chromosomes as visualized by confocal microscopy.

    abstract::Knob heterochromatin served as the model for the development of fluorescent chromosome in situ hybridization on maize meiotic chromosomes. The meiotic chromosomes were hybridized with a digoxigenin-labeled RNA probe of the knob repeat sequence that is a component of the morphologically determined knob heterochromatin....

    journal_title:BioTechniques

    pub_type:

    doi:

    authors: Makowski ER,Ruzin SE

    更新日期:1994-02-01 00:00:00

  • A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis.

    abstract::A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:

    authors: Fernández-Silva P,Enriquez JA,Montoya J

    更新日期:1992-04-01 00:00:00

  • High-quality RNA and DNA from flow cytometrically sorted human epithelial cells and tissues.

    abstract::Microarray technologies have made possible comprehensive analyses of nucleic acid sequence and expression. However, the technology to obtain efficiently high-quality RNA and DNA suitable for array analysis from purified populations of neoplastic cells from human tissues has not been well addressed. Microdissection can...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/02324rr06

    authors: Barrett MT,Glogovac J,Prevo LJ,Reid BJ,Porter P,Rabinovitch PS

    更新日期:2002-04-01 00:00:00

  • Optimized multiplex IgH/ras PCR: a tool for quantitative monitoring of B-lymphoproliferative disorders.

    abstract::The use of quantitative PCR is recommended to monitor the level of residual hematological malignancies. The proposed multiplex IgH/ras PCR uses a co-amplification of the clonal CDR3 rearrangement of the immunoglobulin heavy chain gene (IgH) as a disease marker and a segment of the Hras 1 gene containing codon 61 (ras)...

    journal_title:BioTechniques

    pub_type: 杂志文章

    doi:10.2144/00284st08

    authors: Slavícková A,Ullmannová V,Klener P

    更新日期:2000-04-01 00:00:00