Abstract:
:A system has been established to produce in vitro IgG specific for cell wall determinants of S. aureus by human peripheral blood mononuclear cells (PBMC). PBMC of healthy individuals, of patients with S. aureus infections and of patients with other bacterial infections were cultured for 12 days. In the culture supernatants (SN) total IgG was determined by a competitive RIA, and IgG to purified cell walls (PCW) of S. aureus strain H by a two step ELISA. PBMC of 11 healthy persons produced anti-PCW IgG upon stimulation by pokeweed mitogen (PWM). This indicates the presence in some healthy persons of circulating B cells which can be induced in vitro to synthesize PCW specific IgG. PBMC of S. aureus infected patients, however, synthesized anti-PCW IgG in culture medium alone. This is the first description of spontaneous in vitro production of specific IgG during a bacterial infection and may be analogous to short phases of spontaneous specific IgG production described after immunizations and viral infections. Finally, compared to healthy individuals, an increased total IgG synthesis in vitro by PBMC obtained from patients with S. aureus and from patients with other severe bacterial infections was found. It is concluded that this polyclonal B cell activation has been initiated in vivo. Its biological significance is unknown.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Hauser C,Wilhelm JA,Matter L,Schopfer Ksubject
Has Abstractpub_date
1984-07-01 00:00:00pages
227-33issue
1eissn
0009-9104issn
1365-2249journal_volume
57pub_type
杂志文章abstract::In humans and in laboratory animals, administration of a single does of glucocorticosteroids induces a rapid and transient decrease in the numbers of peripheral blood lymphocytes through a redistribution of circulating lymphocytes. This lymphocytopenic effect is an important factor in the immunosuppressive capacity of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1990.tb03310.x
更新日期:1990-06-01 00:00:00
abstract::To investigate the possible role of tryptophan metabolism in immune regulation of primary Sjögren's syndrome (pSS) the serum concentrations of tryptophan and its metabolite kynurenine were measured by reverse-phase high-performance liquid chromatography (HPLC) in 103 patients with pSS, 56 patients with sicca symptoms ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2005.02889.x
更新日期:2005-10-01 00:00:00
abstract::Nasal administration of microg doses of acetylcholine receptor (AChR) is effective in preventing the development of B cell-mediated EAMG in the Lewis rat, a model for human MG. In order to investigate whether nasal administration of AChR modulates ongoing EAMG, Lewis rats were treated nasally with AChR 2 weeks after i...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1998.00521.x
更新日期:1998-03-01 00:00:00
abstract::To elucidate the in vivo mechanisms involved in the impairment in pulmonary defence as the result of treatment with glucocorticoids, we established fatal pneumonia with bacteraemia in dexamethasone (DEX)-treated mice by means of an intratracheal challenge of Pseudomonas aeruginosa. An increased neutrophil influx was o...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01656.x
更新日期:2001-11-01 00:00:00
abstract::Autoantibodies directed against cytoplasmic antigens of neutrophils (ANCA), especially those with specificity for proteinase 3 (PR-3) and myeloperoxidase, are valuable markers for differential diagnosis and monitoring of disease activity in Wegener's granulomatosis (WG) and other vasculitides. Till now, several concep...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06110.x
更新日期:1994-09-01 00:00:00
abstract::Immune responses towards malignant plasma cells have clearly been demonstrated in the course of monoclonal B cell dyscrasias and shown to be mostly specific for idiotypic determinants of the monoclonal immunoglobulin (Ig). These responses are specifically efficient against lymphoma cells expressing a membrane form of ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01929.x
更新日期:2002-08-01 00:00:00
abstract::As Q fever is associated with an inflammatory syndrome, we determined circulating levels of inflammatory cytokines, cytokine antagonists, and activation markers of leucocytes in patients with acute Q fever and Q fever endocarditis. Tumour necrosis factor (TNF) and IL-6, but not IL-1beta, were markedly increased compar...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00786.x
更新日期:1999-01-01 00:00:00
abstract::Previous studies have shown that the efficiency of phagocytosis is a function of cell cycle and that phagocytosis promotes cell cycle progression. Because phagocytosis is dependent on cellular receptors we hypothesized that Fcgamma receptors (FcgammaR) and complement receptors (CR) expression varied with cell cycle. C...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2006.03132.x
更新日期:2006-08-01 00:00:00
abstract::Lymphocytes isolated from Graves' and Hashimoto thyroid tissue by enzymatic (dispase) digestion or mechanical disaggregation were markedly different in terms of their ability to synthesize thyroid autoantibodies in culture. Dispase digestion, followed by removal of thyroid follicular cells, gave a lymphocyte populatio...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Plasmacytoid dendritic cells (pDCs) are of crucial importance in immune regulation and response to microbial factors. In multiple sclerosis (MS), pDCs from peripheral blood showed an immature phenotype, but its role in susceptibility to MS is not determined. Because infectious diseases are established triggers of exac...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2009.03964.x
更新日期:2009-09-01 00:00:00
abstract::Neopterin is primarily synthesized and released by activated macrophages/monocytes upon stimulation with interferon-γ and is considered as a marker for macrophage activation. This study aimed to analyze the serum levels of neopterin in patients with dermatomyositis (DM) in association with clinical manifestations, lab...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.13404
更新日期:2020-03-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02180.x
更新日期:2003-06-01 00:00:00
abstract::Parasite antigens released from Trypanosoma cruzi-infected cells were adsorbed to infected and uninfected mammalian cells thus rendering them susceptible to immune lysis by antibody and cell-mediated immunity directed against the parasite. BALB/c mice infected with T. cruzi for 15 days developed cytotoxic T lymphocyte...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-04-01 00:00:00
abstract::Whilst the mechanism by which Helicobacter pylori causes different gastroduodenal diseases is uncertain, strains producing the cytotoxin-associated protein (CagA) have greater pathogenicity. Hsps are immunogenic molecules induced by inflammatory mediators. The aim of this study was to assess pathogenicity of hsp antib...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1998.00608.x
更新日期:1998-06-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1988-01-01 00:00:00
abstract::It is generally accepted that multiple sclerosis (MS) is mediated by autoreactive T cells and that myelin basic protein (MBP) is one of the target autoantigens. The T-cell response to MBP has been analysed extensively, largely through the use of T-cell lines (TCL) and T-cell clones (TCC), and to date, three immunodomi...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01831.x
更新日期:2002-06-01 00:00:00
abstract::The role of suppressive anti-retroviral therapy (ART) in eliciting restoration of dysregulated immune function remains unclear in HIV-1 infection. Also, due to tailoring of therapeutic regimens towards HIV-1, this possible impairment of therapy may be even more pronounced in HIV-2 and dual (HIV-D) infection. Thus, we ...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章
doi:10.1111/cei.13342
更新日期:2019-11-01 00:00:00
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journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2249.2011.04461.x
更新日期:2012-01-01 00:00:00
abstract::The production of anti-tetanus toxoid (TT) antibody and anti-anti-TT (auto-anti-Id) antibody has been measured in three young and three old persons. Both antibodies were measured using ELISA. We found that: (i) the anti-TT response of old subjects was lower than that of young subjects, (ii) serum auto-anti-Id antibody...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1993.tb05965.x
更新日期:1993-04-01 00:00:00
abstract::In vitro delayed hypersensitivity was studied in patients with either the chronic or the indeterminate phase of Chagas' disease. Normal healthy individuals were used as controls. The leucocyte migration inhibition test was applied using an antigen an extract of culture forms of T. cruzi or an extract of normal rat hea...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-11-01 00:00:00
abstract::Fifty sera which gave ring-like nuclear staining in immunofluorescence on rat liver tissue sections were characterized. Using immunoprecipitation and Western blotting we showed that 10 of the sera contained antibodies to 200 kD polypeptide(s) of nuclear envelope. Clinical and biological data were available for nine of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::Humoral antibodies to tumour associated membrane antigens of cultured human breast cancer cell lines were studied using the immune adherence (IA) test. Sera from 353 post-operative breast cancer patients and from twenty-five patients immunized by allogeneic breast cancer cells were tested against the MDA-MB-436 cell l...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
abstract::Anaemia of chronic disease (ACD) is frequently found in rheumatoid arthritis (RA). In the pathogenesis of ACD both cytokines, such as tumour necrosis factor-alpha (TNF-alpha), IL-1 and IL-6 as well as a relative deficiency of erythropoietin (EPO), are thought to play a key role. In the present study the role of IL-6 i...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1996.d01-622.x
更新日期:1996-02-01 00:00:00
abstract::In a study of 75 alveolar cell co-cultures from 55 HIV-seropositive subjects, p24 antigen production was identified by ELISA in 11 out of 26 (42%) of unseparated cell cultures, 15 out of 39 (38%) purified alveolar macrophage cultures and eight out of 10 purified alveolar lymphocyte samples. Positivity in unseparated c...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Tolerogenic dendritic cells (DCs) play a critical role in the induction of regulatory T cells (Tregs ), which in turn suppress effector T cell responses. We have previously shown the induction of DCs from human and mouse monocytic cell lines, mouse splenocytes and human peripheral blood monocytes by a novel apolipopro...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12370
更新日期:2014-09-01 00:00:00
abstract::A serial prospective study of cell-mediated immunity to rabbit liver-specific lipoprotein (RLSP) has been done in 26 patients with acute viral hepatitis (AH) (18 HBsAg+ and eight HBsAg-) using a lymphocyte transformation test. An increased stimulation index was recorded in 56% of HBsAg+ cases and in 63% of the HBsAg- ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The alpha 7 nicotinic receptor is reportedly a key element in the cholinergic anti-inflammatory pathway. Because a prototypical ligand for this receptor is nicotine, we studied the in vivo human response to bacterial endotoxin or lipopolysaccharide (LPS) in the context of nicotine or placebo pretreatment. Twelve adult...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1365-2249.2006.03248.x
更新日期:2007-01-01 00:00:00
abstract::One hundred and sixty-six unrelated children and adolescents having growth-onset, insulin-dependent diabetes mellitus (IDD) were studied for pancreatic-cytoplasmic islet cell antibodies (ICA) at various times from the diagnosis of the disease. A strong association between HLA antigens A9 and Bw16 and an absence of ICA...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::Suppression of the lymphocyte response to concanavalin A (Con A), phytohaemagglutinin (PHA) and protein A from Staphylococcus aureus (SpA) by Con A-induced suppressor cells was measured in twenty-four patients with recently active-recovering multiple sclerosis (MS), twelve with inactive MS and twenty-three healthy con...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-04-01 00:00:00
abstract::Here we describe the presence of IgG antibodies, in the sera of patients presenting with insulin-dependent diabetes mellitus (IDDM), that react in Western blots with a 60-kD protein (Mr 60K) from rat hepatic microsomal extracts. Sera from 60 IDDM patients were screened and 31.6% were positive for the Mr 60K band. This...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2000.01366.x
更新日期:2000-11-01 00:00:00