Abstract：
:Humoral antibodies to tumour associated membrane antigens of cultured human breast cancer cell lines were studied using the immune adherence (IA) test. Sera from 353 post-operative breast cancer patients and from twenty-five patients immunized by allogeneic breast cancer cells were tested against the MDA-MB-436 cell line. Fifty-five (15.6%) sera samples from the non-vaccinated group and 131 (77.3%) of 168 sera samples from the immunotherapy group were IA-positive to this cell line after absorption with bovine erythrocytes to exclude antibody to heterologous membrane antigens (HM Ag). Forty-five of the 55 positive-sera from the non-immunized group and 113 of the 131 positive sera from the immunized group became IA-negative after further absorption with lymphoblastoid cells autologous to MDA-MB-436. Subsequently, the twenty-eight positive sera remaining sere tested for oncofetal antigens (OFA). After absorption with OFA rich tissues (fetal brain and M14 melanoma cells), no reactivity remained in the sera samples. In order to identify antibodies specific to breast cancer antigens, the 129 sera samples from non-immunized patients were tested against four other breast cancer cell lines; MDA-MB-157, MDA-MB-231, MCF-7 and UCLASO-B1. Four sera which reacted to more than three of the cell lines were identified. The reactivity of three of the four was due to anti-OFA antibody. The last serum sample was reactive to anti-HLA antibodies. These results indicate that sera of patients with breast cancer contain antibodies to OFA, but do not detect breast histologic type specific antigens as tested by IA using five breast cancer cultured cell lines.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Higuchi M,Robinson DS,Cailleau R,Irie RF,Morton DLsubject
Has Abstractpub_date
1980-01-01 00:00:00pages
90-6issue
1eissn
0009-9104issn
1365-2249journal_volume
39pub_type
杂志文章abstract：:GVHD is a major complication in allogeneic bone marrow transplantation (BMT). MHC class I mismatching increases GVHD, but in MHC-matched BMT minor histocompatibility antigens (mH) presented by MHC class I result in significant GVHD. To examine the modification of GVHD in the absence of cell surface MHC class I molecul...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00578.x
更新日期：1998-05-01 00:00:00
abstract：:A method is described which can be used to quantitate class II MHC antigens (HLA-DR) expressed by cells within tissue sections. A mouse anti-human HLA-DR monoclonal antibody is directly conjugated to the fungal enzyme glucose oxidase. The enzyme, in the presence of its substrate can be used to reduce tetrazolium salts...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:Th1 cell-induced anti-mycobacterial immunity is lost during a progressive Mycobacterium tuberculosis infection in a susceptible host. This study was designed to test the mechanism of the loss of anti-mycobacterial cell-mediated immune response. We demonstrate that M. tuberculosis infection results in increased Fas exp...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00755.x
更新日期：1999-02-01 00:00:00
abstract：:The precise factor responsible for the altered immunological reactivity and diminished inflammatory responses occurring in pregnancy is unknown. Recent observations implicate the pregnancy-associated alpha-2 glycoprotein (PAG). Using rosette assays for the detection of cells carrying Fc receptors and for the demonstra...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:Five out of 51 sera (10%) from patients with progressive systemic sclerosis reacted by immunoblotting with tissue concanavalin A binding glycoproteins of 50 kD and 45 kD Mr, whereas only one out of 133 control sera gave the same reaction (P less than 0.001). The antigens were localized to the microsomal fractions of t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Serum from 98 Thai adults infected with Plasmodium falciparum were examined for the presence of autoantibodies. Malarial containing antibodies sera were also revealed positive for anti-nuclear antibodies with fluorescence speckled pattern, anti-smooth muscle antibodies, anti-mitochondria antibodies and rheumatoid fact...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
abstract：:Serum factors activating the alternative pathway of complement in vitro, independent of classical pathway activation was demonstrated in six of eleven patients with systemic lupus erythematosus (SLE). These serum factors were detected by lysis of gluthathione-sensitized human erythrocytes and by C3 and factor B conver...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：:One hundred and sixty-six unrelated children and adolescents having growth-onset, insulin-dependent diabetes mellitus (IDD) were studied for pancreatic-cytoplasmic islet cell antibodies (ICA) at various times from the diagnosis of the disease. A strong association between HLA antigens A9 and Bw16 and an absence of ICA...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:Interferons play an important, but incompletely understood role in HIV-related disease. We investigated the effect of HAART on plasma levels of IFN-alpha, IFN-gamma, neopterin and interferon-inducible protein 10 (IP-10) in 41 HIV-infected patients during 78 weeks of therapy. At baseline HIV-infected patients had raise...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01144.x
更新日期：2000-03-01 00:00:00
abstract：:In the present study we examined the role of thymic stromal lymphopoietin (TSLP) in experimental autoimmune encephalomyelitis (EAE). Here, we report that TSLP knock-out (KO) mice display a delayed onset of disease and an attenuated form of EAE. This delayed onset was accompanied by a reduced number of encephalitogenic...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12621
更新日期：2015-07-01 00:00:00
abstract：:In patients with ANCA-associated vasculitis (AAV), CD25 expression is increased on circulating T cells. Although in animal experiments the role of CD4(+) CD25(+) T-regulatory-cells (T(reg)) in protection against autoimmunity is well established, the role of these cells in AAV is unknown. To investigate the hypothesis ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02731.x
更新日期：2005-04-01 00:00:00
abstract：:Primary biliary cholangitis (PBC) is a multi-factorial disease caused by the interaction of both genetic predisposition and environmental triggers. Bacterial infection has been investigated most intensively, both epidemiologically and experimentally, as a prime environmental aetiology in PBC. The association of recurr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.13198
更新日期：2019-01-01 00:00:00
abstract：:The potential value of interleukin 1 (IL-1) containing supernatants as a radioprotective agent was evaluated. It was found that the response of irradiated thymocytes to mitogens was partially restored if IL-1 containing supernatants was included in the culture medium immediately after irradiation. A delay of 24 h in t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:In this study we demonstrate that IgG present in the sera of patients with primary Sjögren's syndrome (PSS) could bind and activate muscarinic acetylcholine receptors (mAChRs) of rat parotid gland. These antibodies were able to inhibit in a non-competitive manner the binding of 3H-quinuclidinyl benzilate (QNB) to mACh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1996.42748.x
更新日期：1996-06-01 00:00:00
abstract：:Kawasaki disease (KD) is the leading cause of acquired heart disease of children in North America. It is characterized by a massive immune activation and multi-system vasculitis, which evolves into a site-specific inflammatory response focused at the coronary arteries. Coronary artery (CA) inflammation leads to elasti...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2009.03949.x
更新日期：2009-08-01 00:00:00
abstract：:Interspecies immunization of marmosets with platelets leads to an acute thrombocytopenia which appears to be immunologically mediated. The thrombocytopenia is preceded by the formation of antibodies to the donor platelet antigen and deposition of IgG on the host's platelets. Examination of sera obtained from these ani...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-01-01 00:00:00
abstract：:By using a flow cytometric technique which allows direct identification of proliferating cells within mixed cell populations, we have previously described that soluble extracts obtained from Mycobacterium tuberculosis or M. avium represent strong stimuli for human gammadelta+ T cells. In the present study, we demonstr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00540.x
更新日期：1998-04-01 00:00:00
abstract：:The susceptibility of hepatocytes from patients with chronic hepatitis B to complement-dependent cytotoxicity mediated by heterologous antibodies to hepatitis B virus core (anti-HBc) and surface (anti-HBs) antigens and to hepatic asialoglycoprotein receptor was examined using a microcytotoxicity assay. The anti-HBc-in...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb03658.x
更新日期：1995-05-01 00:00:00
abstract：:IL-10 and IL-12 are cytokines which are important in regulating immune responses. Plasma levels of IL-10 and autoantibodies against double-stranded DNA (dsDNA) often mirror disease activity in patients with SLE. IL-12 secretion from SLE patients' blood mononuclear cells also correlates with disease activity, but has a...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01466.x
更新日期：2001-04-01 00:00:00
abstract：:In humans and in laboratory animals, administration of a single does of glucocorticosteroids induces a rapid and transient decrease in the numbers of peripheral blood lymphocytes through a redistribution of circulating lymphocytes. This lymphocytopenic effect is an important factor in the immunosuppressive capacity of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb03310.x
更新日期：1990-06-01 00:00:00
abstract：:Antibody levels specific for capsular polysaccharides of Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) and to tetanus toxoid (TT), were measured in serum samples of 750 age-stratified subjects from the UK. The study subjects comprised healthy adult volunteers and hospitalized children undergoing ele...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb07959.x
更新日期：1993-08-01 00:00:00
abstract：:We have previously demonstrated enhanced synovial fluid (SF) antigen-presenting cell (APC) function in inflammatory arthritis patients selected on the basis of marked SF mononuclear cell (MNC) responsiveness to reactive arthritis-associated bacteria (Clin Exp Immunol 1990; 79:189-94). In this study we have assessed wh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb03388.x
更新日期：1993-05-01 00:00:00
abstract：:Fourteen cadaver kidney donors were pretreated with a rapid infusion of 5 g of cyclophosphamide and 5 g of methylprednisolone in an attempt to reduce graft immunogenicity by altering immunogenic "passenger" leucocytes. The effects of this drug infusion on peripheral lymphocytes were monitored by measuring the ability ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-09-01 00:00:00
abstract：:A complement fixing, non-organ specific IgG autoantibody is described in 29 patients. The autoantibody gives a highly characteristic, granular staining of liver cells, proximal kidney tubules and stomach surface epithelium. By studies with various subcellular fractions from rat liver, employing two different technique...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:The hypothesis that a virus might in some way be involved in the causation of type 1 diabetes has a long history, but decades of research have failed to resolve the issue beyond reasonable doubt. Viruses could potentially play a primary role in the pathogenesis of type 1 diabetes by initiating autoimmunity, a secondar...
journal_title：Clinical and experimental immunology
pub_type：
doi：10.1111/j.1365-2249.2011.04553.x
更新日期：2012-04-01 00:00:00
abstract：:Isoelectric focusing of serial bleeds from patients with Hashimoto's thyroiditis was carried out and thyroglobulin (Tg) autoantibodies visualized using 125I-labelled Tg followed by autoradiography. Although the spectrotype of these antibodies was polyclonal and varied from patient to patient, each individual's spectro...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:CBA/KlJms-lprcg/lprcg mice with a novel mutation producing systemic lymphoproliferation were investigated for their serological and histological characteristics. The mutant mice showed elevated levels of serum immunoglobulin, C1q-binding immune complexes and antibodies to nuclear antigens such as dsDNA and ssDNA and p...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05138.x
更新日期：1990-01-01 00:00:00
abstract：:Interleukin-6 (IL-6, BSF-2 or IFN-beta 2) is thought to be the major regulator of the acute-phase protein response that follows tissue injury and inflammation, with interleukin-1 (IL-1), tumour necrosis factor and more recently, LIF or HSF III, slightly stimulatory on only certain acute phase proteins. The synthesis o...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05666.x
更新日期：1991-03-01 00:00:00
abstract：:Common variable immunodeficiency (CVID) is the most frequent symptomatic primary immunodeficiency disease, characterized by low levels of circulating immunoglobulins and recurrent bacterial infections, particularly of the respiratory tract. T cell dysfunction is often present, and lymphoproliferative and autoimmune di...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03086.x
更新日期：2006-06-01 00:00:00
abstract：:The lymphotoxin-beta receptor (LTbetaR) pathway is critical for maintenance of organized lymphoid structures and is involved in the development of colitis. To investigate the mechanisms by which LTbetaR activation contributes to the pathology of chronic inflammation we used a soluble LTbetaR-Ig fusion protein as a com...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2004.02402.x
更新日期：2004-04-01 00:00:00
