Thymic stromal lymphopoietin deficiency attenuates experimental autoimmune encephalomyelitis.


:In the present study we examined the role of thymic stromal lymphopoietin (TSLP) in experimental autoimmune encephalomyelitis (EAE). Here, we report that TSLP knock-out (KO) mice display a delayed onset of disease and an attenuated form of EAE. This delayed onset was accompanied by a reduced number of encephalitogenic T helper type 1 (Th1) cells in the central nervous system (CNS) of TSLP KO mice. In addition, CD4(+) and CD8(+) T cells from CNS of TSLP KO mice show a reduced activation status in comparison to wild-type mice. It is noteworthy that we could also show that lymph node cells from TSLP KO mice expanded less efficiently and that interleukin (IL)-6-, interferon (IFN)-γ and tumour necrosis factor (TNF)-α levels were reduced. Furthermore, CD3(+) T cells isolated in the preclinical phase from myelin oligodendrocyte glycoprotein peptide 35-55 (MOG(35-55))-immunized TSLP KO mice showed a reduced response after secondary exposure to MOG(35-55), indicating that differentiation of naive T cells into MOG(35-55)-specific effector and memory T cells was impaired in KO mice. The addition of recombinant TSLP enhanced T cell proliferation during MOG(35-55) restimulation, showing that T cells also respond directly to TSLP. In summary, these data demonstrate that expression of, and immune activation by, TSLP contributes significantly to the immunopathology of EAE.


Clin Exp Immunol


Eckhardt J,Döbbeler M,König C,Kuczera K,Kuhnt C,Ostalecki C,Zinser E,Mak TW,Steinkasserer A,Lechmann M




Has Abstract


2015-07-01 00:00:00












  • A minimally hypomorphic mutation in Btk resulting in reduced B cell numbers but no clinical disease.

    abstract::Reduced B cell numbers and a mutation in Btk are considered sufficient to make the diagnosis of X-linked agammaglobulinaemia. In the process of conducting family studies, we identified a 58-year-old healthy man with an amino acid substitution, Y418H, in the adenosine-5'-triphosphate binding site of Btk. Immunofluoresc...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Conley ME,Farmer DM,Dobbs AK,Howard V,Aiba Y,Shurtleff SA,Kurosaki T

    更新日期:2008-04-01 00:00:00

  • Cellular localization of complement C3 and C4 transcripts in intestinal specimens from patients with Crohn's disease.

    abstract::It has been suggested that the increase in C3 and C4 levels in jejunal perfusates of patients with Crohn's disease (CD) results from local intestinal synthesis of complement. The present study evaluated the expression of these complement genes in inflamed tissues from patients with CD. Surgically resected specimens fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Laufer J,Oren R,Goldberg I,Horwitz A,Kopolovic J,Chowers Y,Passwell JH

    更新日期:2000-04-01 00:00:00

  • Anti-V3 antibody reactivity correlates with clinical stage of HIV-1 infection and with serum neutralizing activity.

    abstract::During HIV-1 infection, the current tenet is that only anti-gag antibodies decline, while those directed at env remain stable. Among the latter antibodies, those directed to the V3 domain of gp120 are assumed to play a role in the immune surveillance against HIV-1. We investigated the correlation between anti-V3 antib...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Fenouillet E,Blanes N,Benjouad A,Gluckman JC

    更新日期:1995-03-01 00:00:00

  • Enhancement of spontaneous killer cytotoxicity by soluble factor.

    abstract::Two-stage stimulation of SK activity of normal human peripheral blood lymphocytes by soluble factors was demonstrated. The first stage was initiated by factors present in supernatant derived from normal B-LCL cultures. Only cell lines which could induce SK activity in culture in an MLR-type reaction had the capacity t...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hofman FM,Spina CA

    更新日期:1980-12-01 00:00:00

  • Anti-neutrophil monoclonal antibody therapy inhibits the development of adjuvant arthritis.

    abstract::The aim of this study was to determine the contribution of neutrophils to adjuvant arthritis (AA) by in vivo depletion of peripheral blood neutrophils. Specific anti-neutrophil MoAb, RP3 (10 mg), or a control antibody was given twice daily on days 8-11 after injection of Mycobacterium tuberculosis in inbred male Sprag...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Santos LL,Morand EF,Hutchinson P,Boyce NW,Holdsworth SR

    更新日期:1997-02-01 00:00:00

  • Programmed death-1 ligands 1 and 2 expression in cutaneous squamous cell carcinoma and their relationship with tumour- infiltrating dendritic cells.

    abstract::The programmed death-1 (PD-1) receptor ligands, PD-L1 and PD-L2, are co-stimulatory molecules that contribute to the negative regulation of T lymphocyte activation. It is still unclear whether there is correlation between PD-L1 or PD-L2 and tumour-infiltrating dendritic cells (TIDCs) in cutaneous squamous cell carcino...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jiao Q,Liu C,Li W,Li W,Fang F,Qian Q,Zhang X

    更新日期:2017-06-01 00:00:00

  • Interaction of endothelial cells and neutrophils in vitro: kinetics of thrombomodulin, intercellular adhesion molecule-1 (ICAM-1), E-selectin, and vascular cell adhesion molecule-1 (VCAM-1): implications for the relevance as serological disease activity m

    abstract::Recently markers of endothelial cell activation or injury gained increasing interest as serological parameters of disease activation in vasculitides. Among these, soluble serum thrombomodulin, ICAM-1, VCAM-1 and E-selectin are of particular interest. However, only thrombomodulin showed the expected close correlation. ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Boehme MW,Raeth U,Scherbaum WA,Galle PR,Stremmel W

    更新日期:2000-01-01 00:00:00

  • After chemotherapy, functional humoral response capacity is restored before complete restoration of lymphoid compartments.

    abstract::Chemotherapy has, besides the beneficial effects, several adverse effects. Suppression of the immune system is one of the most important problems. Infections caused by encapsulated bacteria like Streptococcus pneumoniae are responsible for a major part of infectious problems during and after treatment. The splenic mar...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zandvoort A,Lodewijk ME,Klok PA,Breukels MA,Rijkers GT,Timens W

    更新日期:2003-01-01 00:00:00

  • Abnormal liver function in common variable immunodeficiency disorders due to nodular regenerative hyperplasia.

    abstract::Patients with common variable immunodeficiency disorders are monitored for liver function test abnormalities. A proportion of patients develop deranged liver function and some also develop hepatomegaly. We investigated the prevalence of abnormalities and types of liver disease, aiming to identify those at risk and det...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ward C,Lucas M,Piris J,Collier J,Chapel H

    更新日期:2008-09-01 00:00:00

  • Asbestos related changes in pulmonary and systemic immune responses--early enhancement followed by inhibition.

    abstract::We recently developed an animal model in order to study the pulmonary and systemic reactions following exposure to environmental pollutants. In this model, conscious sheep received repeated intratracheal instillations of a suspension of 0 (controls), 2mg (low dose) or 128 mg (high dose) of chrysotile asbestos fibres o...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Rola-Pleszczynski M,Lemaire I,Sirois P,Massé S,Bégin R

    更新日期:1982-08-01 00:00:00

  • Epitope shift of proteinase-3 anti-neutrophil cytoplasmic antibodies in patients with small vessel vasculitis.

    abstract::Anti-neutrophil cytoplasmic antibodies against proteinase 3 (PR3-ANCA) are used as diagnostic tools for patients with small vessel vasculitis (AASV). We have produced chimeric mouse/human PR3 molecules and investigate changes in reactivity over time and the possible relationship between epitope specificity and clinica...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,多中心研究


    authors: Selga D,Segelmark M,Gunnarsson L,Hellmark T

    更新日期:2010-06-01 00:00:00

  • Macrophages are a source of extracellular adenosine deaminase-2 during inflammatory responses.

    abstract::Serum activity of the adenosine deaminase (ADA) isozyme, ADA2, has been reported to be elevated during various disease states. Macrophages have been suggested as the cellular source of extracellular ADA activity because they are one of the only cell types in which intracellular ADA2 activity has been measured, but ext...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Conlon BA,Law WR

    更新日期:2004-10-01 00:00:00

  • Bispecific anti-human red blood Rhesus-D antigen x anti Fc gamma RI targeted antibody-dependent cell-mediated cytotoxicity and phagocytosis by mononuclear leucocytes.

    abstract::The Fc receptor mediated antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis induced by bispecific antibody (BsAb) to the high-affinity Fc receptor for IgG (Fc gamma RI) and to human red blood group antigen RhD were studied in vitro, using human mononuclear leucocytes as effector cells. The results w...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Deramoudt EX,Gilard C,Lepine N,Alonso JM,Romet-Lemonne JL

    更新日期:1992-08-01 00:00:00

  • Specificities of antibody to acetylcholine receptor in rabbits with experimental myasthenia gravis.

    abstract::The injection of acetylcholine receptor (AChR) purified from Narke electroplax japonica induced 'experimental autoimmune myasthenia gravis' (EAMG) in rabbits. Serial measurements of anti-AChR antibody titre using Narke AChR and rabbit AChR as antigen revealed that the intensity of myasthenia had a rather closer correl...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ueno S,Kang J,Takeuchi H,Takahashi M,Tarui S

    更新日期:1980-07-01 00:00:00

  • Murine models of transplantation tolerance through mixed chimerism: advances and roadblocks.

    abstract::Organ transplantation is the treatment of choice for patients with end-stage organ failure, but chronic immunosuppression is taking its toll in terms of morbidity and poor efficacy in preventing late graft loss. Therefore, a drug-free state would be desirable where the recipient permanently accepts a donor organ while...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Mahr B,Wekerle T

    更新日期:2017-08-01 00:00:00

  • Complement-induced mixed aggregation of T and B lymphocytes and myeloid cells and the effect of adjuvant.

    abstract::Complement induces mixed aggregation of murine spleen cells bearing C3 receptors. In the present studies, we show with immunofluorescent techniques that these aggregates are formed by T and B lymphocytes and myeloid cells, B cells constituting the predominant component in normal mice. There are striking changes in the...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Arnaiz-Villena A,Roitt IM

    更新日期:1975-07-01 00:00:00

  • Association of increased platelet-associated immunoglobulins with thrombocytopenia and the severity of disease in secondary dengue virus infections.

    abstract::Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue haemorrhagic fever (DHF). To develop a better understanding of the roles of platelet-associated IgG (PAIgG) and IgM (PAIgM) in inducing thrombocytopenia and its severity of disease in patients with secondary dengue viru...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Saito M,Oishi K,Inoue S,Dimaano EM,Alera MT,Robles AM,Estrella BD Jr,Kumatori A,Moji K,Alonzo MT,Buerano CC,Matias RR,Morita K,Natividad FF,Nagatake T

    更新日期:2004-11-01 00:00:00

  • IgE is expressed on, but not produced by, fetal cells in the human placenta irrespective of maternal atopy.

    abstract::The prevalence of atopic diseases in children has increased during the last decades. Atopic symptoms usually appear early in life. This implies an early priming for atopic disease, possibly even at the fetal level. We therefore compared the presence and production of IgE in the local in utero environment during pregna...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sverremark Ekstrom E,Nilsson C,Holmlund U,van der Ploeg I,Sandstedt B,Lilja G,Scheynius A

    更新日期:2002-02-01 00:00:00

  • Evidence that the Mitsuda reaction to Mycobacterium leprae can be mediated by lymphocytes responsive to Mycobacterium tuberculosis.

    abstract::A positive Mitsuda skin test for delayed type hypersensitivity to Mycobacterium leprae is associated with a high level of protection against lepromatous leprosy, while the value of tuberculin sensitivity in leprosy is less pronounced. Cutaneous lymphocytes, isolated from the Mitsuda reaction of a PPD-positive individu...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Roberts PP,Dockrell HM,McAdam KP

    更新日期:1988-06-01 00:00:00

  • Paneth cell granule depletion in the human small intestine under infective and nutritional stress.

    abstract::Paneth cells are important contributors to the intestinal antimicrobial barrier through synthesis and release of antimicrobial peptides and proteins. Animal studies indicate that Paneth cell numbers, location and granule morphology are altered by infection and zinc status. We examined human tissue to determine whether...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kelly P,Feakins R,Domizio P,Murphy J,Bevins C,Wilson J,McPhail G,Poulsom R,Dhaliwal W

    更新日期:2004-02-01 00:00:00

  • Differing surface marker characteristics in plasma cell dyscrasias with particular reference to IgM myeloma.

    abstract::Immunological marker studies using direct immunofluorescence and rosette methods were performed on the bone marrow and peripheral blood of an IgM myeloma, and on the peripheral blood of two cases of IgA myeloma and one IgG myeloma. These studies confirmed the peripheral blood involvement in plasma cell dyscrasias. In ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Burns GF,Cawley JC,Barker CR,Worman CP,Raper CG,Hayhoe FG

    更新日期:1978-03-01 00:00:00

  • Relationships between humoral factors in HIV-1-infected mothers and the occurrence of HIV infection in their infants.

    abstract::Based on what is known about the biology of HIV-1 vertical transmission, the HIV burden of the mother, maternal immune factors and the integrity of the placental barrier are likely to play major roles. We therefore sought to determine whether the presence of antibodies in sera from 47 HIV-1-infected mothers, including...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mabondzo A,Rouvier P,Raoul H,Le Naour R,Courpotin C,Hervé F,Parnet-Mathieu F,Lasfargues G,Dormont D

    更新日期:1995-12-01 00:00:00

  • Regulation of cytokine responses by seasonality of vitamin D status in healthy individuals.

    abstract::The immune modulating capacity of vitamin D(3) is well-recognized. Ultra-violet (UV) exposure determines production of vitamin D(3) in vivo and varies through the course of the year, especially in temperate regions. However, it is not known whether the human innate immune response differs due to seasonality. To valida...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Khoo AL,Chai LY,Koenen HJ,Sweep FC,Joosten I,Netea MG,van der Ven AJ

    更新日期:2011-04-01 00:00:00

  • Phenotypic and functional characterization of the circulating NK compartment in hairy cell leukaemia.

    abstract::A phenotypic and functional analysis of the circulating natural killer (NK) cell population was carried out in a series of patients with hairy cell leukaemia (HCL). The overall mean NK activity of both the mononuclear and T cell fractions was reduced compared to that of normal controls (466 lytic units (lu) v 573 lu a...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Foa R,Lauria F,Lusso P,Raspadori D,Fierro MT,Tazzari PL,Caudana L,Matera L

    更新日期:1986-05-01 00:00:00

  • Predominant type-2 response in infants with respiratory syncytial virus (RSV) infection demonstrated by cytokine flow cytometry.

    abstract::Acute RSV infection in infancy may produce some asthma-like symptoms and may be followed by a recurrent wheeze later in childhood. It has been proposed that RSV infection stimulates type-2 cytokine responses, resembling those found in atopy and asthma. Peripheral blood cells were obtained from RSV-infected infants (n ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Bendelja K,Gagro A,Bace A,Lokar-Kolbas R,Krsulovic-Hresic V,Drazenovic V,Mlinaric-Galinovic G,Rabatic S

    更新日期:2000-08-01 00:00:00

  • Induction of the in vitro anti-HBs response by hepatitis B surface antigen.

    abstract::The production in vitro of antibody to hepatitis B surface antigen by peripheral blood mononuclear cells of healthy volunteers was studied after each of the three doses of hepatitis B vaccine. An in vitro hepatitis B surface antigen antibody response was successfully induced in 12% of the specimens taken over a 7 mont...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Filion LG,Saginur R

    更新日期:1988-12-01 00:00:00

  • Parietal cell autoantibodies: localization of their binding sites on isolated gastric glands.

    abstract::The localization of antigens reacting with parietal cell antibodies found in the sera of some patients with pernicious anaemia was studied by the indirect immunoperoxidase method on viable gastric glands isolated from the mouse or human fundus by collagenase digestion. We observed that only the luminal surface of pari...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Duchateau A,Zeitoun P

    更新日期:1983-08-01 00:00:00

  • Generation of activated lymphocytes. Analysis of giant SRBC rosettes.

    abstract::Giant SRBC rosette-forming cells were detected in samples of mitogen-stimulated human peripheral blood lymphocytes. When the concentrations of mitogens were varied, the amount of [3H]TdR incorporated by the lymphocytes also varied. In general, the higher the amount of [3H]TdR incorporated, the higher were the percenta...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Yu DT

    更新日期:1976-12-01 00:00:00

  • Translational mini-review series on Toll-like receptors: Toll-like receptor ligands as novel pharmaceuticals for allergic disorders.

    abstract::Characterization of the Toll-like receptor (TLR) family and associated signalling pathways provides a key molecular basis for our understanding of the relationship between exposure to microbial products and susceptibility to immune-mediated disorders. Indeed, ligation of TLR controls innate and adaptive immune respons...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章,评审


    authors: Goldman M

    更新日期:2007-02-01 00:00:00

  • T cell subsets in leprosy lesions: in situ characterization using monoclonal antibodies.

    abstract::Cryostat sections of dermal lesions from 30 untreated leprosy patients were studied by indirect immunofluorescence using monoclonal antibodies defining T cell subsets and Ia like antigens. Most lymphocytes in leprosy lesions were positive for OKT3 and Ia like antigens indicating thereby the presence of activated T cel...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Narayanan RB,Bhutani LK,Sharma AK,Nath I

    更新日期:1983-03-01 00:00:00