Abstract:
:PM0188 is a newly identified sialyltransferase from P. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (CMP-NeuAc) to an acceptor sugar. Although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. Here, we report the X-ray crystal structures of PM0188 in the presence of an acceptor sugar and a donor sugar analogue, revealing the precise mechanism of sialic acid transfer. Site-directed mutagenesis, kinetic assays, and structural analysis show that Asp141, His311, Glu338, Ser355 and Ser356 are important catalytic residues; Asp141 is especially crucial as it acts as a general base. These complex structures provide insights into the mechanism of sialyltransferases and the structure-based design of specific inhibitors.
journal_name
BMB Repjournal_title
BMB reportsauthors
Kim DU,Yoo JH,Lee YJ,Kim KS,Cho HSdoi
10.5483/bmbrep.2008.41.1.048subject
Has Abstractpub_date
2008-01-31 00:00:00pages
48-54issue
1eissn
1976-6696issn
1976-670Xjournal_volume
41pub_type
杂志文章相关文献
BMB Reports文献大全abstract::The Wnt signaling pathway is well known to play major roles in skeletal development and homeostasis. In certain aspects, fracture repair mimics the process of bone embryonic development. Thus, the importance of Wnt signaling in fracture healing has become more apparent in recent years. Here, we summarize recent resear...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2014.47.12.193
更新日期:2014-12-01 00:00:00
abstract::Cell therapies utilizing mesenchymal stem cells (MSCs) have a great potential in many research and clinical settings. The mechanisms underlying the therapeutic effects of MSCs have been studied previously and the paracrine effects elicited by their production of various growth factors and cytokines were recognized as ...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2014.47.8.106
更新日期:2014-08-01 00:00:00
abstract::Cellular senescence is a state of permanent cell cycle arrest which exhibits large and flattened morphological characteristics. Cellular senescence might evolve to a beneficial process to suppress the accumulation of severely damaged cells. However, senescent cells are considered as the cause of age-related pathologie...
journal_title:BMB reports
pub_type: 社论
doi:
更新日期:2019-01-01 00:00:00
abstract::The interaction of the rod GTP binding protein, Transducin (G(t)), with bleached Rhodopsin (R(*)) was investigated by measuring radiolabeled guanine nucleotide binding to and release from soluble and/or membrane-bound G(t) by reconstituting G(t) containing bound GDP (G(t-)GDP) or the hydrolysis-resistant GTP analog gu...
journal_title:BMB reports
pub_type: 杂志文章
doi:
更新日期:2008-07-31 00:00:00
abstract::The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys6...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2009.42.11.764
更新日期:2009-11-30 00:00:00
abstract::Mac-2BP is a ligand of the galectin family that has been suggested to affect tumor proliferation and metastasis formation. We assessed Mac-2BP expression at the transcriptional and translational levels to evaluate nerve growth factor (NGF)-induced Mac-2BP expression. A time kinetic analysis using reverse transcription...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2008.41.11.784
更新日期:2008-11-30 00:00:00
abstract::Methylglyoxal (MG) was identified as an intermediate in non-enzymatic glycation and increased levels were reported in patients with diabetes. In this study, we evaluated the effects of MG on the modification of ferritin. When ferritin was incubated with MG, covalent crosslinking of the protein increased in a time- and...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2012.45.3.147
更新日期:2012-03-01 00:00:00
abstract::MED19 is a member of the Mediator that plays a key role in the activation and repression of signal transduction or the regulation of transcription in carcinomas. To tested the functional role of MED19 in human prostate cancer, we downregulated MED19 expression in prostate cancer cells (PC-3 and DU145) by lentivirus-me...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2011.44.8.547
更新日期:2011-08-01 00:00:00
abstract::High mobility group box 1 (HMGB1) is involved in the pathogenesis of vascular diseases. Unlike activated protein C (APC), the activation of PAR-1 by thrombin is known to elicit proinflammatory responses. To determine whether the occupancy of EPCR by the Gla-domain of APC is responsible for the PAR-1-dependent antiinfl...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2013.46.11.056
更新日期:2013-11-01 00:00:00
abstract::Monoacylglycerol acyltransferase 1 (MGAT) is a microsomal enzyme that catalyzes the synthesis of diacylglycerol (DAG) and triacylglycerol (TAG). However, the subcellular localization and catalytic function domain of this enzyme is poorly understood. In this report, we identified that murine MGAT1 localizes to the endo...
journal_title:BMB reports
pub_type: 新闻
doi:10.5483/bmbrep.2017.50.7.036
更新日期:2017-07-01 00:00:00
abstract::p13 gene was first described in Leucania separata multinuclear polyhedrosis virus (Ls-p13) several years ago, but the function of P13 protein has not been experimentally investigated to date. In this article, we indicated that the expression of p13 from Heliothis armigera single nucleocapsid nucleopolyhedrovirus (Ha-p...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2012.45.12.058
更新日期:2012-12-01 00:00:00
abstract::Inter-cellular communication via diffusible small molecules is a defining character not only of multicellular forms of life but also of single-celled organisms. A large number of bacterial genes are regulated by the change of chemical milieu mediated by the local population density of its own species or others. The ce...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/BMBRep.2011.44.1.1
更新日期:2011-01-01 00:00:00
abstract::Fibrin is a natural provisional matrix found in wound healing, while type I collagen is a major organic component of bone matrix. Despite the frequent use of fibrin and type I collagen in bone regenerative approaches, their comparative efficacies have not yet been evaluated. In the present study, we compared the effec...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2014.47.2.156
更新日期:2014-02-01 00:00:00
abstract::Cohesin is a multi-protein complex composed of four core subunits (SMC1A, SMC3, RAD21, and either STAG1 or STAG2) that is responsible for the cohesion of sister chromatids following DNA replication until its cleavage during mitosis thereby enabling faithful segregation of sister chromatids into two daughter cells. Rec...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2014.47.6.092
更新日期:2014-06-01 00:00:00
abstract::Our understanding of the relationships between genes, brains, and behaviors has changed a lot since the first behavioral mutants were isolated in the fly bottles of the Benzer lab at Caltech (1), but Drosophila is still an excellent model system for studying the neurobiology of behavior. Recent advances provide an unp...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2009.42.11.705
更新日期:2009-11-30 00:00:00
abstract::In the last decade, we have witnessed an unprecedented clinical success in cancer immunotherapies targeting the programmed cell-death ligand 1 (PD-L1) and programmed cell-death 1 (PD-1) pathway. Besides the fact that PD-L1 plays a key role in immune regulation in tumor microenvironment, recently a plethora of reports ...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2021-01-01 00:00:00
abstract::As the prevalence of obesity has increased explosively over the last several decades, associated metabolic disorders, including type 2 diabetes, dyslipidemia, hypertension, and cardiovascular diseases, have been also increased. Thus, new strategies for preventing and treating them are needed. The nuclear peroxisome pr...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2014.47.11.174
更新日期:2014-11-01 00:00:00
abstract::The N-myc downstream regulated gene (NDRG) family members are dysregulated in several tumors. Functionally, NDRGs play an important role in the malignant progression of cancer cells. However, little is known about the potential implications of NDRG4 in pancreatic ductal adenocarcinoma (PDAC). The aim of the current st...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2020-12-01 00:00:00
abstract::The importance of lipids in cell signaling and tissue physiology is demonstrated by the many CNS pathologies involving deregulated lipid metabolism. One such critical metabolic event is the activation of phospholipase A(2) (PLA(2)), which results in the hydrolysis of membrane phospholipids and the release of free fatt...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2008.41.8.560
更新日期:2008-08-31 00:00:00
abstract::The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 Beta production in bronchoalveolar lavage fluid (BA...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2010.43.4.257
更新日期:2010-04-01 00:00:00
abstract::Tunicamycin, an endoplasmic reticulum (ER) stress inducer, specifically inhibits N-glycosylation. The cyclic AMP (cAMP) response element-binding protein H (CREBH) was previously shown to be regulated by UPR-dependent proteolytic cleavage in the liver. On the other hand, the role of CREBH in other tissues is unknown. I...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2011.44.11.735
更新日期:2011-11-01 00:00:00
abstract::Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this study, we identified ...
journal_title:BMB reports
pub_type: 杂志文章
doi:
更新日期:2019-02-01 00:00:00
abstract::Viruses have evolved to manipulate the host cell machinery for virus propagation, in part by interfering with the host cellular signaling network. Molecular studies of individual pathways have uncovered many viral host-protein targets; however, it is difficult to predict how viral perturbations will affect the signali...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2012.45.4.213
更新日期:2012-04-01 00:00:00
abstract::Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid β-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. ...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2019-09-01 00:00:00
abstract::Basically, cancer is a disease initiated by mutations in the genome. However, cancer is regarded not only as a genetical disease but also an immunological disease because cancer cells can be targeted by the immune system. The immune system recognizes mutated proteins as non-self, foreign antigens, so-called neoantigen...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2021-01-01 00:00:00
abstract::Recent studies have reported that delayed-rectifier Kv channels regulate apoptosis in the nervous system. Herein, we investigated changes in the expression of the delayed-rectifier Kv channels Kv1.2, Kv2.1, and Kv3.1 after acute spinal cord injury (SCI) in rats. We performed RT-PCR analysis and found an increase in th...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2010.43.11.756
更新日期:2010-11-01 00:00:00
abstract::Glucose effects on the vegetative growth of Dictyostelium discoideum Ax2 were studied by examining oxidative stress and tetrahydropteridine synthesis in cells cultured with different concentrations (0.5X, 7.7 g L(-1); 1X, 15.4 g L(-1); 2X, 30.8 g L(-1)) of glucose. The growth rate was optimal in 1X cells (cells grown ...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2013.46.2.128
更新日期:2013-02-01 00:00:00
abstract::GROWTH-REGULATING FACTORs (GRFs) are sequencepecific DNA-binding transcription factors that regulate various aspects of plant growth and development. GRF proteins interact with a transcription cofactor, GRF-INTERACTING FACTOR (GIF), to form a functional transcriptional complex. For its activities, the GRF-GIF duo requ...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:
更新日期:2019-04-01 00:00:00
abstract::L-2-Oxothiazolidine-4-carboxylic acid (OTC) is a cysteine prodrug that maintains glutathione in tissues. The present study was designed to investigate anti-fibrotic and anti-oxidative effects of OTC via modulation of nuclear factor erythroid 2-related factor 2 (Nrf2) in an in vivo thioacetamide (TAA)-induced hepatic f...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2012.45.6.276
更新日期:2012-06-01 00:00:00
abstract::Weight loss ≥ 5 percent is sufficient to significantly reduce health risks for obese people; therefore, development of novel weight loss compounds with reduced toxicity is urgently required. After screening of natural compounds with antiadipogenesis properties in 3T3-L1 cells, we determined that kahweol, a coffee-spec...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2017.50.11.031
更新日期:2017-11-01 00:00:00