Abstract:
:Cell therapies utilizing mesenchymal stem cells (MSCs) have a great potential in many research and clinical settings. The mechanisms underlying the therapeutic effects of MSCs have been studied previously and the paracrine effects elicited by their production of various growth factors and cytokines were recognized as being crucial. However, the molecular controls that govern these paracrine effects remain poorly understood. To elucidate the molecular regulators of this process, we performed a global knockdown of microRNAs (miRNAs) in human adipose-derived mesenchymal stem cells (hADSCs) by inhibiting DGCR8, a key protein in miRNA biogenesis. Global disruption of miRNA biogenesis in hADSCs caused dramatic changes in the expression of subsets of growth factors and cytokines. By performing an extensive bioinformatic analysis, we were able to associate numerous putative miRNAs with these genes. Taken together, our results strongly suggest that miRNAs are essential for the production of growth factors and cytokines in hADSCs.
journal_name
BMB Repjournal_title
BMB reportsauthors
Park SK,Lee JS,Choi EK,You D,Kim CS,Suh Ndoi
10.5483/bmbrep.2014.47.8.106subject
Has Abstractpub_date
2014-08-01 00:00:00pages
469-74issue
8eissn
1976-6696issn
1976-670Xpii
2834journal_volume
47pub_type
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