Abstract:
:Alternative splicing generates several interleukin-6 (IL-6) isoforms; for them an antagonistic activity to the wild-type IL-6 has been proposed. In this study we quantified the relative abundance of IL-6 mRNA isoforms in a panel of mouse tissues and in C2C12 cells during myoblast differentiation or after treatment with the Ca(2+) ionophore A23187, the AMP-mimetic AICAR and TNF-α. The two mouse IL-6 isoforms identified, IL-6δ5 (deletion of the first 58 bp of exon 5) and IL-6δ3 (lacking exon 3), were not conserved in rat and human, did not exhibit tissue specific regulation, were expressed at low levels and their abundance closely correlated to that of full-length IL-6. Species-specific features of the IL-6 sequence, such as the presence of competitive 3' acceptor site in exon 5 and insertion of retrotransposable elements in intron 3, could explain the production of IL-6δ5 and IL-6δ3. Our results argued against biological significance for mouse IL-6 isoforms.
journal_name
BMB Repjournal_title
BMB reportsauthors
Annibalini G,Guescini M,Agostini D,Matteis RD,Sestili P,Tibollo P,Mantuano M,Martinelli C,Stocchi Vdoi
10.5483/bmbrep.2012.45.1.32subject
Has Abstractpub_date
2012-01-01 00:00:00pages
32-7issue
1eissn
1976-6696issn
1976-670Xjournal_volume
45pub_type
杂志文章相关文献
BMB Reports文献大全abstract::Ubiquitin is an essential, highly-conserved small regulatory protein in eukaryotic cells. It covalently modifies a wide variety of targeted proteins in the forms of monomer and polymers, altering the conformation and binding properties of the proteins and thus regulating proteasomal delivery, protein activities and lo...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2008.41.3.177
更新日期:2008-03-31 00:00:00
abstract::Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid β-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. ...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2019-09-01 00:00:00
abstract::Advances in the understanding of the Hippo signaling as a key regulatory pathway of proliferation and apoptosis have provided mechanical insights for controlling organ size and tumorigenicity. Recently, much attention has been directed to the regulation of LATS1/2 (large tumor suppressor) kinases that phosphorylate YA...
journal_title:BMB reports
pub_type: 新闻
doi:10.5483/bmbrep.2017.50.6.078
更新日期:2017-06-01 00:00:00
abstract::Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS) that promote apoptotic cell death. We showed that cytosolic NADP+-dependent isocitrate dehydrogenase (IDPc) plays an essential role in the control of cellular redox balance and defense against oxidative damage, by supplying NADPH for...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2014.47.4.137
更新日期:2014-04-01 00:00:00
abstract::The single-stranded DNA binding activity of the Escherichia coli RecA protein is crucial for homologous recombination to occur. This and other biochemical activities of ssDNA binding proteins may be affected by various factors. In this study, we analyzed the effect of CaCl(2), NaCl and NH(4)NO(3) salts in combination ...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2011.44.5.341
更新日期:2011-05-01 00:00:00
abstract::Liver cells experience hypoxic stress when drug-metabolizing enzymes excessively consume O2 for hydroxylation. Hypoxic stress changes the transcription of several genes by activating a heterodimeric transcription factor called hypoxia-inducible factor- 1α/β (HIF-1α/β). We found that hypoxic stress (0.1% O2) decreased ...
journal_title:BMB reports
pub_type: 新闻
doi:10.5483/bmbrep.2016.49.3.188
更新日期:2016-03-01 00:00:00
abstract::Methylglyoxal (MG) was identified as an intermediate in non-enzymatic glycation and increased levels were reported in patients with diabetes. In this study, we evaluated the effects of MG on the modification of ferritin. When ferritin was incubated with MG, covalent crosslinking of the protein increased in a time- and...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2012.45.3.147
更新日期:2012-03-01 00:00:00
abstract::The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 Beta production in bronchoalveolar lavage fluid (BA...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2010.43.4.257
更新日期:2010-04-01 00:00:00
abstract::As the prevalence of obesity has increased explosively over the last several decades, associated metabolic disorders, including type 2 diabetes, dyslipidemia, hypertension, and cardiovascular diseases, have been also increased. Thus, new strategies for preventing and treating them are needed. The nuclear peroxisome pr...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2014.47.11.174
更新日期:2014-11-01 00:00:00
abstract::Bone morphogenetic protein type 2 receptor (BMPR2) is one of the transforming growth factor-β (TGF-β) superfamily receptors, performing diverse roles during embryonic development, vasculogenesis, and osteogenesis. Human BMPR2 consists of 1,038 amino acids, and contains functionally conserved extracellular, transmembra...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2017.50.6.059
更新日期:2017-06-01 00:00:00
abstract::Tetrahydrobiopterin (BH4) is a pivotal cofactor for enzymes responsible for the synthesis and release of monoamine neurotransmitters including dopamine (DA) and serotonin (5-HT) as well as the release of glutamate (Glu). Deficiencies in BH4 levels and reduced activities of BH(4)-associated enzymes have been recently r...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/BMBRep.2010.43.9.593
更新日期:2010-09-01 00:00:00
abstract::Salsolinol (1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline) is a compound derived from dopamine metabolism and is capable of causing dopaminergic neurodegeneration. Oxidative modification of neurofilament proteins has been implicated in the pathogenesis of neurodegenerative disorders. In this study, oxidative m...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2012.45.2.114
更新日期:2012-02-01 00:00:00
abstract::The Pphsc70 (heat shock cognate 70) gene was isolated from the endoparasitoid Pteromalus puparum and then characterized. The full-length cDNA was 2204 base pair (bp) and contained a single 1968 bp ORF that encoded a polypeptide of 656 amino acids with a predicted molecular mass of 71.28 kDa. Phylogenetic analysis base...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2008.41.5.388
更新日期:2008-05-31 00:00:00
abstract::Translocase of outer mitochondrial membrane 20 (TOMM20) plays an essential role as a receptor for proteins targeted to mitochondria. TOMM20 was shown to be overexpressed in various cancers. However, the oncological function and therapeutic potential for TOMM20 in cancer remains largely unexplored. The purpose of this ...
journal_title:BMB reports
pub_type: 杂志文章
doi:
更新日期:2019-12-01 00:00:00
abstract::The 12 kDa FK506-binding protein (FK506BP12), an immunosuppressor, modulates T cell activation via calcineurin inhibition. In this study, we investigated the ability of PEP-1-FK506BP12, consisting of FK506BP12 fused to the protein transduction domain PEP-1 peptide, to suppress catabolic responses in primary human chon...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2015.48.7.050
更新日期:2015-07-01 00:00:00
abstract::Cell proliferation is a delicately regulated process that couples growth signals and metabolic demands to produce daughter cells. Interestingly, the proliferation of tumor cells immensely depends on glycolysis, the Warburg effect, to ensure a sufficient amount of metabolic flux and bioenergetics for macromolecule synt...
journal_title:BMB reports
pub_type: 杂志文章,评审
doi:10.5483/bmbrep.2014.47.3.273
更新日期:2014-03-01 00:00:00
abstract::Hypoxia is associated with many pathological conditions as well as the normal physiology of metazoans. We identified a lactate-dependent signaling pathway in hypoxia, mediated by the oxygen- and lactate-regulated protein NDRG family member 3 (NDRG3). Oxygen negatively regulates NDRG3 expression at the protein level vi...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2015.48.6.080
更新日期:2015-06-01 00:00:00
abstract::Gene regulation in the brain is essential for long-term plasticity and memory formation. Despite this established notion, the quantitative translational map in the brain during memory formation has not been reported. To systematically probe the changes in protein synthesis during memory formation, our recent study exp...
journal_title:BMB reports
pub_type: 新闻
doi:10.5483/bmbrep.2016.49.4.044
更新日期:2016-04-01 00:00:00
abstract::Tunicamycin, an endoplasmic reticulum (ER) stress inducer, specifically inhibits N-glycosylation. The cyclic AMP (cAMP) response element-binding protein H (CREBH) was previously shown to be regulated by UPR-dependent proteolytic cleavage in the liver. On the other hand, the role of CREBH in other tissues is unknown. I...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2011.44.11.735
更新日期:2011-11-01 00:00:00
abstract::In embryonic stem cells (ESCs), cell cycle regulation is deeply connected to pluripotency. Especially, core transcription factors (CTFs) which are essential to maintaining the pluripotency transcription programs should be reset during M/G1 transition. However, it remains unknown about how CTFs are governed during cell...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2016.49.10.161
更新日期:2016-10-01 00:00:00
abstract::The N-myc downstream regulated gene (NDRG) family members are dysregulated in several tumors. Functionally, NDRGs play an important role in the malignant progression of cancer cells. However, little is known about the potential implications of NDRG4 in pancreatic ductal adenocarcinoma (PDAC). The aim of the current st...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2020-12-01 00:00:00
abstract::Tumor metastasis involves circulating and tumor-initiating capacities of metastatic cancer cells. Hepatic TM4SF5 promotes EMT for malignant growth and migration. Hepatocellular carcinoma (HCC) biomarkers remain unexplored for metastatic potential throughout metastasis. Here, novel TM4SF5/CD44 interaction-mediated self...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2015.48.3.047
更新日期:2015-03-01 00:00:00
abstract::Glucose effects on the vegetative growth of Dictyostelium discoideum Ax2 were studied by examining oxidative stress and tetrahydropteridine synthesis in cells cultured with different concentrations (0.5X, 7.7 g L(-1); 1X, 15.4 g L(-1); 2X, 30.8 g L(-1)) of glucose. The growth rate was optimal in 1X cells (cells grown ...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2013.46.2.128
更新日期:2013-02-01 00:00:00
abstract::Genome-wide association studies (GWAS) have become popular as an approach for the identification of large numbers of phenotype-associated variants. However, differences in genetic architecture and environmental factors mean that the effect of variants can vary across populations. Understanding population genetic diver...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2013.46.8.191
更新日期:2013-08-01 00:00:00
abstract::Matrix metalloproteinase 1 (MMP-1), a calcium-dependent zinccontaining collagenase, is involved in the initial degradation of native fibrillar collagen. Tissue necrosis factor-alpha (TNFα) is a pro-inflammatory cytokine that is rapidly produced by dermal fibroblasts, monocytes/macrophages, and keratinocytes and regula...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2020-06-01 00:00:00
abstract::We cloned and pharmacologically characterized the guinea pig cysteinyl leukotriene (CysLT) 2 receptor (gpCysLT2). gpCysLT2 consists of 317 amino acids with 75.3%, 75.2%, 73.3% identity to those of humans, mice and rats, respectively. The gpCysLT2 gene is highly expressed in the lung, moderately in eosinophils, skin, s...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2008.41.2.139
更新日期:2008-02-29 00:00:00
abstract::Hemistepsin A (HsA) is a guaianolide sesquiterpene lactone that inhibits hepatitis and liver fibrosis. We evaluated the effects of HsA on liver X receptor (LXR)-mediated hepatic lipogenesis in vitro and in vivo. Up to 10 M, HsA did not affect the viability of HepG2 and Huh7 cells. Pretreatment with 5-10 μM HsA signif...
journal_title:BMB reports
pub_type: 新闻
doi:
更新日期:2020-08-26 00:00:00
abstract::The serine/threonine kinase mTOR is essential for the phosphoinositide 3-kinases (PI3K) signaling pathway, and regulates the development and function of immune cells. Aberrant activation of mTOR signaling pathway is associated with many cancers including leukemia. Here, we report the contributions of mTOR signaling to...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/BMBRep.2016.49.1.201
更新日期:2016-01-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is widely-considered to be a modulating factor of anoikis and cancer metastasis. We found that, in MDA-MB-231 cells, TP53I11 (tumor protein P53 inducible protein 11) suppressed EMT and migration in vitro, and inhibited metastasis in vivo. Our findings showed that hypoxic treatme...
journal_title:BMB reports
pub_type: 杂志文章
doi:
更新日期:2019-06-01 00:00:00
abstract::Cell therapies utilizing mesenchymal stem cells (MSCs) have a great potential in many research and clinical settings. The mechanisms underlying the therapeutic effects of MSCs have been studied previously and the paracrine effects elicited by their production of various growth factors and cytokines were recognized as ...
journal_title:BMB reports
pub_type: 杂志文章
doi:10.5483/bmbrep.2014.47.8.106
更新日期:2014-08-01 00:00:00