Abstract:
:The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 Beta production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group || underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). ells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group ||, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 Beta and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.
journal_name
BMB Repjournal_title
BMB reportsauthors
Klimenko OV,Vobruba V,Martasek Pdoi
10.5483/bmbrep.2010.43.4.257subject
Has Abstractpub_date
2010-04-01 00:00:00pages
257-62issue
4eissn
1976-6696issn
1976-670Xjournal_volume
43pub_type
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