Abstract:
:Adipocyte differentiation is a complex developmental process forming adipocytes from various precursor cells. The murine 3T3-L1 preadipocyte cell line has been most frequently used in the studies of adipocyte differentiation. Differentiation of 3T3-L1 preadipocytes includes a medium containing fetal bovine serum (FBS) with hormonal induction. In this study, we observed that differentiation medium containing adult bovine serum (ABS) instead of FBS did not support differentiation of preadipocytes. Impaired adipocyte differentiation was due to the presence of a serum protein factor in ABS that suppresses differentiation of preadipocytes. Using a proteomic analysis, alpha-2-macroglobulin and paraoxonase/arylesterase 1, which were previously shown to suppress differentiation of preadipocytes, were identified as anti-adipogenic proteins. Although their functional mechanisms have not yet been elucidated, the anti-adipogenic effects of these proteins are discussed.
journal_name
BMB Repjournal_title
BMB reportsauthors
Park J,Park J,Nahm SS,Choi I,Kim Jdoi
10.5483/bmbrep.2013.46.12.082subject
Has Abstractpub_date
2013-12-01 00:00:00pages
582-7issue
12eissn
1976-6696issn
1976-670Xpii
2315journal_volume
46pub_type
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