Abstract:
:Determining the genomic organization of the Ovis aries (Ovar) major histocompatibility complex class IIa region is essential for future functional studies related to antigen presentation. In this study, a bacterial artificial chromosome (BAC) library of genomic DNA from peripheral blood leukocytes (PBL) of a Rambouillet ram was constructed, and BAC clone consisting of the major histocompatibility complex (MHC) class II DQB2, DQA2, DQB1, DQA1, and DRB1 loci was identified and completely sequenced. The BAC clone consists of 160,889 bp of finished sequence with the loci arranged in the following order: DQB2, DQA2, DQB1, DQA1, and DRB1 with 14.3, 25, 6.6, and 40.9 Kb spanning between the loci, respectively. All five of these loci were transcribed in the animal used to generate the MHC class II BAC clone. Repeat or retrotransposable elements along with MHC class II cis promoter elements consisting of S, X, and Y boxes were identified in the sequence. In addition, 16 non-coding conserved sequences amongst primates, carnivores, and ruminants were identified (p < 0.001). These conserved sequences include binding sites for transcription factors with known roles in immune cells, and they provide a basis for further functional investigation of the genes in this region. This is the first ruminant finished sequence of the DQB2-DRB1 region, and this sequence information will aid in whole genome and transcriptome analyses of MHC class II.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Herrmann-Hoesing LM,White SN,Kappmeyer LS,Herndon DR,Knowles DPdoi
10.1007/s00251-008-0275-5subject
Has Abstractpub_date
2008-04-01 00:00:00pages
167-76issue
3-4eissn
0093-7711issn
1432-1211journal_volume
60pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::The H-2K glycoprotein from the MHC mutant bm10 was analyzed biochemically to determine where primary structural differences distinguished it from the parental standard molecule, Kb. Comparative peptide maps showed differences in two peptides known to be part of the parental CNBr fragment spanning amino acids 139 to 22...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00364285
更新日期:1983-01-01 00:00:00
abstract::A healthy adult having no serologically detectable HLA class I A locus antigens was identified. The parents of the individual are consanguineous. Results of a family study indicated that the individual is homozygous for the B46-Cw1-DR8.1 haplotype, which was shown to be positively associated with A*0207 in our previou...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00186597
更新日期:1996-01-01 00:00:00
abstract::There have been several attempts over the years to identify positions in the peptide-binding region (PBR) of human leukocyte antigens (HLA) that influence the specificity of bound amino acids (AAs) at each position in the peptide. Originally, six pockets (A-F) were defined by calculating the surface area of the PBR on...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-007-0197-7
更新日期:2007-04-01 00:00:00
abstract::Killer cell immunoglobulin-like receptors (KIRs) represent the most polymorphic genes responsible for natural killer cell function, while human leukocyte antigen (HLA) class I molecules define and restrict cytotoxic T lymphocyte responses. Specific KIR, HLA, or KIR-HLA combinations have been implicated in the outcome ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-016-0920-3
更新日期:2016-10-01 00:00:00
abstract::HLA-B and C are related class I genes which are believed to have arisen by duplication of a common ancestor. Previous study showed the presence of orthologues for both HLA-B and C in African apes but only for HLA-B in Asian apes. These observations suggested that the primate C locus evolved subsequent to the divergenc...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050566
更新日期:1999-09-01 00:00:00
abstract::KIR2DL5 alleles were physically linked to alleles at adjacent KIR loci to define this region of KIR haplotypes in 55 gene-positive random African Americans. The majority carried KIR2DL5B. Three KIR2DL5A and six KIR2DL5B alleles that have been previously described and 11 novel KIR2DL5 alleles were identified by DNA seq...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0458-8
更新日期:2010-08-01 00:00:00
abstract::Assembly of HLA class I molecules was studied using pulse-chase labeling of B-lymphoblastoid cell lines with 35S-methionine, immunoprecipitation with antibodies detecting free or beta 2-microglobulin-associated heavy chain and isoelectric focusing. Marked differences between the products of different class I alleles w...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216831
更新日期:1993-01-01 00:00:00
abstract::As genetically engineered mutant mice deficient in single genes are usually generated on a C57BL/6 background, to study mast cell trafficking in mutant mice, we initially investigated whether mast cells accumulated in bronchi in C57BL/6 mice challenged with OVA allergen acutely or chronically for 1 to 3 months. The to...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0452-1
更新日期:2010-08-01 00:00:00
abstract::Using mouse bone marrow-derived macrophages we determined the role of interferon (IFN)-gamma at the different steps in expression of the I-A alpha chain of MHC class II molecules, from transcription to the cell surface. Levels of transcription, RNA, and protein were low in cells not stimulated with IFN-gamma. Treatmen...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510100312
更新日期:2001-03-01 00:00:00
abstract::Mammalian interleukin-4 (IL-4) and IL-13 are T helper type 2 (Th2) cytokines with pleiotropic functions in immunity. They signal through receptors containing IL-4Rα and IL-2Rγ or IL-13Rα1. In addition, a decoy receptor, IL-13Rα2, is known to exist and modulates the function of IL-13. The existence of fish orthologues ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0508-2
更新日期:2011-04-01 00:00:00
abstract::The organization of the major histocompatibility complex (MHC) class II region in cattle was investigated by Southern blot analysis using human probes corresponding to DO, DP, DQ, and DR genes. Exon-specific probes were also employed to facilitate the assessment of the number of different bovine class II genes. The re...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00351084
更新日期:1988-01-01 00:00:00
abstract::The role of H-2- and T-region products in determining allogeneic cell rejection was evaluated in H-2 congenic and recombinant mice by transplanting A1ATH and A6ATL leukemia cell lines induced in A.TH and A.TL strains, respectively, by Moloney murine leukemia virus.--In K- or D-region incompatible hosts transplant fail...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01561686
更新日期:1981-03-01 00:00:00
abstract::The inbred strain STS/A exhibits a higher proliferative response in the mixed lymphocyte culture (MLC) to stimulator cells of all 11 tested inbred mouse strains with 10 different major histocompatibility complex (MHC) haplotypes, as well as to stimulation with IL-2 than does the strain BALB/cHeA. However, alloantigen-...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF02602654
更新日期:1996-01-01 00:00:00
abstract::Antigenic peptide binding to MHC class II molecules in the endocytic pathway occurs via a multifactorial process that requires the support of a specialized lysosomal chaperone called HLA-DM. DM shows both in primary amino acid sequence and quaternary structure a high homology to both MHC class I and class II molecules...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s002510000208
更新日期:2000-08-01 00:00:00
abstract::Rh-related transcripts present in bone marrow samples from several species of nonhuman primates (chimpanzee, gorilla, gibbon, crab-eating macaque) have been amplified by RT-polymerase chain reaction using primers deduced from the sequence of human RH genes. Nucleotide sequence analysis of the nonhuman transcripts reve...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00172151
更新日期:1995-01-01 00:00:00
abstract::T cell receptor genes are assembled in developing T lymphocytes from discrete V, D, and J genes by a site-specific somatic rearrangement mechanism. A flanking recombination signal, composed of a conserved heptamer and a semiconserved nonamer separated by 12 or 23 variable nucleotides, targets the activity of the rearr...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0150-1
更新日期:2006-11-01 00:00:00
abstract::The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which spe...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510000227
更新日期:2000-09-01 00:00:00
abstract::Natural killer (NK) cells constitute the third major population of lymphocytes. They possess the inherent capacity to kill various tumor and virally infected cells and mediate the rejection of bone-marrow grafts in lethally irradiated animals. A large family of NK cell receptors belong to the C-type lectin superfamily...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050679
更新日期:1999-10-01 00:00:00
abstract::Protein tyrosine phosphorylation is an important regulatory mechanism in cell physiology. While the protein tyrosine kinase (PTKase) family has been extensively studied, only six protein tyrosine phosphatases (PTPases) have been described. By Southern blot analysis, genomic DNA from several different phyla were found ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00211693
更新日期:1991-01-01 00:00:00
abstract::In an effort to generate an A.CA mouse expressing Ed, the Ead gene has been introduced into A.CA mice which lack the major histocompatibility complex (MHC) class II E molecule. Flow cytometric analysis shows cell surface expression of the E alpha chain on lymphocytes and macrophages in the transgenic mice. Analysis of...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01719237
更新日期:1991-01-01 00:00:00
abstract::Multiple sclerosis (MS) is a common disease of the central nervous system characterized by myelin loss and progressive neurological dysfunction. An underlying genetic susceptibility plays a clear role in the etiology of MS, likely acting in concert with an undefined environmental exposure. Full-genome screenings in mu...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050621
更新日期:2000-04-01 00:00:00
abstract::The genomic organisation of the major histocompatibility complex (MHC) seems to vary considerably between different bird species. In order to understand this variation it is important to gather information from different species. We have, for the first time, investigated MHC class II polymorphism in a wader species, t...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0503-3
更新日期:2003-01-01 00:00:00
abstract::Since the discovery of human leukocyte antigens (HLAs), the function of major histocompatibility complex (MHC) gene families in a wide range of diseases have been the subject of research for decades. In particular, the associations of autoimmune disorders to allelic variants and candidate genes encoding the MHC are we...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s00251-021-01204-x
更新日期:2021-02-02 00:00:00
abstract::Several lines of evidence have confirmed the importance of Nod2 mutations for disease susceptibility in Crohn's disease. For tracing Nod2 evolution, exons 4a, 4e, 8, and 12 mutations were screened in a collection of 1,064 DNA samples from 52 worldwide populations. The overall allele frequency was 7.5% for single nucle...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-008-0274-6
更新日期:2008-02-01 00:00:00
abstract::Immunoglobulin-A deficiency (IgA-D) is the most common human Ig class deficiency with an estimated frequency of approximately 1 in 500 in the Swedish population. We investigated the immunoglobulin heavy chain constant region gene segments (IGHC) in 103 individuals with IgA-D and the immunoglobulin heavy chain variable...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00218046
更新日期:1992-01-01 00:00:00
abstract::The peptide repertoire presented on human leukocyte antigen (HLA) class I molecules is largely determined by the structure of the peptide binding groove. It is expected that the molecules having similar grooves (i.e., belonging to the same supertype) might present similar/overlapping peptides. However, the extent of p...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0552-6
更新日期:2011-11-01 00:00:00
abstract::A retrovirus element (TLev1) is located within the Thymus leukemia antigen (Tla) locus of the C57BL/10 mouse major histocompatibility complex. Low-copy probes have been isolated from sequences flanking the TLev1 integration site to examine the distribution of TLev1 among inbred mouse strains having genotypically deter...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00345501
更新日期:1988-01-01 00:00:00
abstract::The ability of local ultraviolet B (UVB) irradiation to impair the induction of dinitrofluorobenzene (DNFB)-specific contact hypersensitivity (CH) in mice has been shown to be genetically determined. We have explored the possibility that the mouse Tnfa and Lps loci are involved. We demonstrate that C3H/HeN (Lpsn) stra...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00241633
更新日期:1990-01-01 00:00:00
abstract::Many immunoglobulin variable region (IgV) genes are present in the vertebrate genome and provide a basis for antibody diversity. IgV genes have been classified into distinct families according to DNA sequence similarity. Comparisons of VH and VL genes from two mammalian species (mouse and human) have led to the conclu...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00188428
更新日期:1995-01-01 00:00:00
abstract::Immunogenicity for T cell-independent B-cell response assessed by splenic plaque-forming cell (PFC) response and cell-surface expression measured by laser flow cytometry of various class I H-2 antigens on mouse red blood cells (RBC) were compared. It was found that the order of magnitude of both immunogenicity and cel...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372295
更新日期:1986-01-01 00:00:00