Abstract:
:The gene for the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is responsible for severe combined immune deficiency (SCID) and its products associate with Ku70/Ku86 autoantigens, c-Abl, and other factors to exert its roles. Investigations to date suggest that DNA-PKcs comprises several regions which specifically interact with these known and other as yet unidentified factors. Nevertheless, the relationship between the structure and function of the DNA-PKcs molecule is poorly understood. Here we report cloning of the entire DNA-PKcs cDNA from chicken and Xenopus laevis. Comparative study of the DNA-PKcs polypeptides from four different vertebrates revealed at least three novel conserved regions in addition to the carboxyl-terminal region containing the phosphatidylinositol-3 kinase domain. We also demonstrated that the four vertebrates share the common genomic organization between a licensing factor, MCM4, and DNA-PKcs, both of which locate in a head-to-head manner within a few kilobase intervals. These data provide useful clues for the further genetic study of this huge multifunctional enzyme.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Fujimori A,Araki R,Fukumura R,Ohhata T,Takahashi H,Kawahara A,Tatsumi K,Abe Mdoi
10.1007/s002510000227subject
Has Abstractpub_date
2000-09-01 00:00:00pages
965-73issue
11eissn
0093-7711issn
1432-1211journal_volume
51pub_type
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