Abstract:
:Transgenic mice were produced in which human HLA-Cw4 is stably integrated, behaves as a single Mendelian trait, and, being under the transcriptional control of human CD2, is selectively and efficiently expressed in T lymphocytes. These mice were used as a model system to determine whether HLA-type C molecules can be exposed on the surface of activated lymphocytes as free heavy chains, non-associated with beta2-microglobulin (beta2m). In our transgenic mice we could identify HLA-Cw4 molecules either as free chains or as beta2m-associated molecules by the use of monoclonal antibodies specific for either conformation of HLA class I and nonreactive to mouse H2 molecules. Resting mouse lymphocytes were shown by western transfer analysis to contain sizeable amounts of HLA-Cw4 free chains, but they exposed on their surface HLA-Cw4 only in association with beta2m, as indicated by flow cytometric measurements. Conversely, where the content of total HLA-Cw4 was increased, lectin-activated mouse lymphocytes exposed on their outer cell membrane HLA-Cw4 molecules in both conformations, namely, also as free heavy chains. Isoelectrofocusing analysis confirmed the presence of both HLA-Cw4 molecular conformations in activated T cells and indicated that HLA-Cw4 heavy chains can bind to mouse beta2m with the same low affinity displayed for human beta2m. The results of our experiments led us to conclude that (1) association with beta2m is not necessary for the exposure of HLA-C on the surface of activated T lymphocytes and (2) cell activation affects the balance between the two conformational forms of HLA-C.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Aiuti A,Forte P,Simeoni L,Lino M,Pozzi L,Fattorossi A,Giacomini P,Ginelli E,Beretta A,Siccardi Adoi
10.1007/BF00179398subject
Has Abstract,Author List Incompletepub_date
1995-01-01 00:00:00pages
368-75issue
5eissn
0093-7711issn
1432-1211journal_volume
42pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::Wild mice captured in Texas, Scotland, Federal Republic of Germany, Denmark, Spain, Greece, Israel, Egypt, and Chile were mated to inbred strains and through successive backcross matings and H-2 typing lines homozygous for wild-derived H-2, haplotypes were established. The lines, which are neither congenic nor inbred,...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00952959
更新日期:1983-01-01 00:00:00
abstract::Position 45 represents a highly polymorphic residue within HLA class I alleles, which contacts the p2 position of bound peptides in 85% of the peptide-HLA structures analyzed, while the neighboring residues 41 and 46 are not involved in peptide binding. To investigate the influence of residue 45 at the functional leve...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0583-z
更新日期:2012-03-01 00:00:00
abstract::Mutation rates of H2 and non-H2 histocompatibility genes in the mouse are examined over a 25-year period. Detected by skin graft rejections, the mutations were screened in inbred and hybrid mice from a continuously maintained and monitored colony and from a regularly supplied set of mice provided from the National Can...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050325
更新日期:1997-01-01 00:00:00
abstract::The immune response of the rat to group A streptococcal carbohydrate (SACHO) and an associated idiotype, Id-1, was used to examine the effect of paternal immunity on Id-1 and SACHO-specific antibody expression by the offspring. First litters, conceived before immunization of the father, had significantly higher Id-1 l...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00372236
更新日期:1985-01-01 00:00:00
abstract::Many fishes express high levels of intraspecific variability, often linked to resource partitioning. Several studies show that a species' evolutionary trajectory of adaptive divergence can undergo reversals caused by changes in its environment. Such a reversal in neutral genetic and morphological variation among lake ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-017-0996-4
更新日期:2018-01-01 00:00:00
abstract::Human leucocyte antigen (HLA)-DRB1*15 is associated with predisposition to multiple sclerosis (MS), although conjecture surrounds the possible involvement of an alternate risk locus in the class I region of the HLA complex. We have shown previously that an alternate MS risk allele(s) may be encompassed by the telomeri...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0183-5
更新日期:2007-03-01 00:00:00
abstract::Virus-specific CD8(+) T cells play an important role in controlling HIV/SIV replication. These T cells recognize intracellular pathogen-derived peptides displayed on the cell surface by individual MHC class I molecules. In the SIV-infected rhesus macaque model, five Mamu class I alleles have been thoroughly characteri...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0541-9
更新日期:2011-12-01 00:00:00
abstract::The A15, L6, and S5.7(TAPA1) proteins are members of the transmembrane 4 superfamily (TM4SF). The A15 is expressed in immature human T cells and in the human brain. The MXS1(CCG-B7) gene which codes for A15 contains triplet nucleotide repeats which have been associated with neuropsychiatric diseases such as Huntington...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00189229
更新日期:1994-01-01 00:00:00
abstract::We cloned the cDNAs and genes of two different types of toll-like receptors from Japanese flounder. The results of homology searches suggested that these genes (designated JF-TLR2 and JF-TLR22) are homologues of human TLR2 and fugu TLR22, respectively. The cDNAs of JF-TLR2 and JF-TLR22 encoded 818 and 961 amino acid r...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0657-2
更新日期:2004-04-01 00:00:00
abstract::Genes of the major histocompatibility complex (MHC) have been characterized in all extant lineages of mammals. The tammar wallaby (Macropus eugenii) is well established as a model marsupial species; however, no classical MHC sequences have been described from this species. We have isolated two MHC class II beta-chain ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0644-7
更新日期:2004-02-01 00:00:00
abstract::Coeliac disease is strongly associated with HLA-DQ2, but it is possible that additional major histocompatibility complex genes also confer disease susceptibility. Encoded close to HLA-DQ are two genes, TAP1 and TAP2, whose products are believed to transport antigenic peptides from the cytoplasm into the endoplasmic re...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00210476
更新日期:1993-01-01 00:00:00
abstract::Experimental infection of Mauritian cynomolgus macaques by simian immunodeficiency virus is a representative model of HIV infection, currently in favour for evaluating the efficacy of new preventive or curative treatments. Extensive studies of major histocompatibility complex (MHC) polymorphism by microsatellites reve...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0504-6
更新日期:2011-05-01 00:00:00
abstract::One mouse with a known recombination (NAK) at the Igk locus on chromosome 6 and two new recombinants [B6.PL (75NS) and B6.PL (85NS)] were examined using a series of probes, each of which is specific for a set of immunoglobulin (Ig) Vk genes. Under high stringency conditions, each probe detects from 1 to 19 Bam HI rest...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00373021
更新日期:1986-01-01 00:00:00
abstract::Over a period of some 20 years, Werner Eugen Mayer played a significant role in establishing a framework for molecular studies of Mhc genes in multiple vertebrates. His work largely concerned gene isolation, sequencing, and related bioinformatic analyses both for the Mhc and for immune system genes of about 200 specie...
journal_title:Immunogenetics
pub_type: 传,历史文章,杂志文章,评审
doi:10.1007/s00251-012-0654-9
更新日期:2012-12-01 00:00:00
abstract::The non-major histocompatibility complex (MHC)-encoded CD1 family has recently emerged as a new antigen-presenting system that is distinct from either MHC class I or class II molecules. In the present study, we determined the genomic structure of the rat CD1 locus. It was extremely similar to mouse CD1 genes, especial...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050396
更新日期:1998-06-01 00:00:00
abstract::A graft-vs-host reaction (GVHR) develops after major histocompatibility complex (MHC)-compatible bone marrow-transplantation. In the genetic combination studied, B10.D2 donor cells differed from those of (DBA/2 x B10.D2)F1 mice for multiple DBA/2 minor histocompatibility antigens (mHAg) and minor lymphocyte stimulatin...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00241258
更新日期:1994-01-01 00:00:00
abstract::Insect bite hypersensitivity (IBH) is a chronic allergic dermatitis common in horses. Affected horses mainly react against antigens present in the saliva from the biting midges, Culicoides ssp, and occasionally black flies, Simulium ssp. Because of this insect dependency, the disease is clearly seasonal and prevalence...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0573-1
更新日期:2012-03-01 00:00:00
abstract::Endemic pemphigus foliaceus (EPF), is an autoimmune disease associated with production of IgG antibodies against epidermal antigens. We have tested 38 patients and 50 control subjects living in endemic areas to investigate whether HLA genes are associated with host factors that determine whether or not exposed individ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216698
更新日期:1991-01-01 00:00:00
abstract::The H-2K glycoprotein from the MHC mutant bm10 was analyzed biochemically to determine where primary structural differences distinguished it from the parental standard molecule, Kb. Comparative peptide maps showed differences in two peptides known to be part of the parental CNBr fragment spanning amino acids 139 to 22...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00364285
更新日期:1983-01-01 00:00:00
abstract::Natural killer (NK) cells constitute the third major population of lymphocytes. They possess the inherent capacity to kill various tumor and virally infected cells and mediate the rejection of bone-marrow grafts in lethally irradiated animals. A large family of NK cell receptors belong to the C-type lectin superfamily...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050679
更新日期:1999-10-01 00:00:00
abstract::Dendritic cells (DC) are specialist antigen presenting cells which capture antigens in the periphery, migrate centrally, and present the processed antigens in the context of major histocompatibility complex and appropriate co-stimulatory molecules to T lymphocytes for the initiation of an immune response. DEC-205 has ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050381
更新日期:1998-05-01 00:00:00
abstract::Six IgA allotypes are serologically identifiable in inbred mice. The sequences of the PCR-amplified C alpha 1, C alpha 2 and C alpha 3 exons from the genomic DNA of mice of four previously unsequenced allotypes already have been compared with those of BALB/c and of a wild mouse, Mus pahari, in the literature. Sporadic...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0434-z
更新日期:2002-03-01 00:00:00
abstract::Since the discovery of human leukocyte antigens (HLAs), the function of major histocompatibility complex (MHC) gene families in a wide range of diseases have been the subject of research for decades. In particular, the associations of autoimmune disorders to allelic variants and candidate genes encoding the MHC are we...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s00251-021-01204-x
更新日期:2021-02-02 00:00:00
abstract::Using the fluorescence-activated cell sorter (FACS II), we have analyzed the expression of H-2K- and H-2D-gene products on the membrane of various cellular components of the murine immune system. Using this serological technique we show a basic difference between T and B lymphocytes. Whereas all cellular components an...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01567827
更新日期:1980-01-01 00:00:00
abstract::Control of HIV-1 viremia and progression to AIDS has been associated with specific HLA genes. The tumor necrosis factor ( TNF) and the non-classical major histocompatibility (MHC) class I chain-related A ( MICA) genes are located in the genomic segment between the HLA class I and II genes and variants of both genes ha...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0604-7
更新日期:2003-10-01 00:00:00
abstract::Genetic effects on spontaneous autoimmune thyroiditis in chickens were assessed by measuring phenotypic symptoms, the titer of circulating antibody to thyroglobulin, and the pathological change in the thyroids of young chicks. One or more loci within the B complex (the major histocompatibility complex of the chicken) ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01561675
更新日期:1981-01-01 00:00:00
abstract::Genomic copy number variants (CNVs) are a common, heritable source of inter-individual differences in genomic sequence. Their influence on phenotypic variability and their involvement in the pathogenesis of several common diseases is well established and the object of many current studies. In the course of examining C...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0459-7
更新日期:2010-08-01 00:00:00
abstract::We describe a bioinformatic analysis of germline and rearranged immunoglobulin kappa chain (IGK) gene sequences, performed in order to assess the completeness and reliability of the reported IGK repertoire. In contrast to the reported heavy-chain gene repertoire, which includes many dubious sequences, only five IGK va...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-008-0325-z
更新日期:2008-11-01 00:00:00
abstract::We have analyzed genomic DNA in a large number of independent examples of five HLA-DQw2-bearing extended haplotypes for their associated subtypes by restriction fragment length polymorphism (RFLP) using DRB, DQA, and DQB probes after Taq I and Pst I digestion and Southern blotting. In addition to three previously desc...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00210445
更新日期:1990-01-01 00:00:00
abstract::TAP1 and TAP2 are two major histocompatibility complex (MHC) genes, located between HLA-DP and -DQ, whose products form a transporter molecule involved in endogenous antigen processing. Polymorphic residues have been described in both genes and, in this study, we have identified another polymorphic site within the ade...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216802
更新日期:1993-01-01 00:00:00