Abstract:
:We describe a bioinformatic analysis of germline and rearranged immunoglobulin kappa chain (IGK) gene sequences, performed in order to assess the completeness and reliability of the reported IGK repertoire. In contrast to the reported heavy-chain gene repertoire, which includes many dubious sequences, only five IGK variable gene (IGKV) alleles appear to have been reported in error. There was, however, insufficient evidence to justify removing these IGKV genes from the germline repertoire. Bioinformatic analysis of apparent mismatches between reported germline genes and 1,863 expressed IGK sequences suggested the existence of two unreported IGKV polymorphisms. Genomic screening of 12 individuals led to the confirmation of both of these polymorphisms, IGKV1-16*02 and IGKV2-30*02. We also show that in contrast to the heavy chain, the IGK repertoire is dominated by sequences that use just a handful of kappa variable (IGKV) and junction (IGKJ) gene pairs. There is also little modification of IGKV and IGKJ genes by the processes of exonuclease removal and N nucleotide addition. The expressed IGK repertoire therefore lacks diversity and the junction region is particularly constrained. Remarkably, the analysis of a dataset of 435 relatively unmutated rearranged kappa genes showed that ten amino acid sequences account for almost 10% of the rearrangements, with identical sequences being derived from as many as seven independent sources. Such dominant sequences are likely to have important roles in the operation of the humoral immune response.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Collins AM,Wang Y,Singh V,Yu P,Jackson KJ,Sewell WAdoi
10.1007/s00251-008-0325-zsubject
Has Abstractpub_date
2008-11-01 00:00:00pages
669-76issue
11eissn
0093-7711issn
1432-1211journal_volume
60pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract::A large number of HLA-Cw4 (Cw *0402) peptides were purified, sequenced, and identified from breast and ovarian carcinoma cell lines. HLA-Cw4 molecules were expressed in these cells as soluble, secreted HLA (sHLA) and recovered from the growth medium. The peptides were separated by capillary reversed-phase HPLC and ana...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0570-0
更新日期:2003-06-01 00:00:00
abstract::The size of B-cell subsets appears to be under genetic control, but the mechanism of this regulation is unknown. By analyzing five congenic strains of mice that differ only in their H2 haplotype, we addressed the issue of whether the MHC genes are involved in the relative proportions of B-1a, B-1b and B-2 cells. Not o...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-002-0457-5
更新日期:2002-06-01 00:00:00
abstract::The polymorphism of feline MHC antigens was examined using biochemical methods. The following observations were made: (1) feline class I and II antigens are polymorphic. Their biochemical features were established using rabbit and mouse reagents directed against human MHC products; they resemble those observed for oth...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00398799
更新日期:1986-01-01 00:00:00
abstract::Binding of peptides to major histocompatibility complex (MHC) molecules is the single most selective step in the recognition of pathogens by the cellular immune system. The human MHC genomic region (called HLA) is extremely polymorphic comprising several thousand alleles, each encoding a distinct MHC molecule. The pot...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-008-0341-z
更新日期:2009-01-01 00:00:00
abstract::The variability of immune responses modulated by human leukocyte antigen (HLA) genes and secreted cytokines is a significant factor in the development of a protective effect of measles vaccine. We studied the association between type 1 helper T cells (Th1)- and Th2-like cytokine immune responses and HLA class I allele...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-005-0061-6
更新日期:2005-12-01 00:00:00
abstract::Ataxia-telangiectasia (A-T), a rare inherited disorder, usually affects the nervous and immune systems, and occasionally other organs. A-T is associated mainly with mutations in the ataxia telangiectasia mutated (ATM) gene, which encodes a protein kinase that has a major role in the cellular response to DNA damage. We...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-017-0983-9
更新日期:2017-07-01 00:00:00
abstract::To determine the nucleotide polymorphism of activating killer-cell immunoglobulin-like receptors (aKIR) 3DS1 and 2DS3, we developed a novel direct-sequencing method and analyzed DNA samples of 175 KIR3DS1(+) individuals and 72 KIR2DS3(+) individuals from the white population. The putative ligand-binding extracellular ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-007-0239-1
更新日期:2007-10-01 00:00:00
abstract::Virus-specific CD8(+) T cells play an important role in controlling HIV/SIV replication. These T cells recognize intracellular pathogen-derived peptides displayed on the cell surface by individual MHC class I molecules. In the SIV-infected rhesus macaque model, five Mamu class I alleles have been thoroughly characteri...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-011-0541-9
更新日期:2011-12-01 00:00:00
abstract::Antigen-specific CD4+ T cells play a critical role in protective immunity to many infectious pathogens. Although the antigen-specific CD4+ T cells can be measured by functional assays such as proliferation or cytokine enzyme-linked immunospot, such assays are limited to a specific function and cannot quantify anergic ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-006-0140-3
更新日期:2006-09-01 00:00:00
abstract::The sheep immunoglobulin heavy chain Igh-J locus has been characterized in order to determine the genomic organization of JH segments and their contribution to heavy chain diversity. The locus contains six segments, of which two are functional and four are apparently pseudogenes. These segments span a 1.8 kilobase (kb...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050274
更新日期:1997-01-01 00:00:00
abstract::In the present study, we investigate patterns of variation in the KIR cluster in a large and well-characterized sample of worldwide human populations in the Human Genome Diversity Project-Centre d'Etude du Polymorphisme Humain (HGDP-CEPH) panel in order to better understand the patterns of diversity in the region. Com...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-012-0629-x
更新日期:2012-10-01 00:00:00
abstract::We have isolated and characterized cDNAs and genes for pufferfish, Fugu rubripes, (Fugu) orthologues of mammalian interleukin (IL)-12 subunits (IL-12 p35 and IL-12 p40). The deduced amino acid sequences of the Fugu IL-12 subunits showed homology with mammalian IL-12 subunits (p35: 50.4-58.0% similarity; p40: 51.2-55.4...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0582-9
更新日期:2003-08-01 00:00:00
abstract::Codon bias and base composition in major histocompatibility complex (MHC) sequences have been studied for both class I and II loci in Homo sapiens and Pan troglodytes. There is low to moderate codon bias for the MHC of humans and chimpanzees. In the class I loci, the same level of moderate codon bias is seen for HLA-B...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s002510050493
更新日期:1999-04-01 00:00:00
abstract::Presentation of peptide antigens by MHC-II proteins is prerequisite to effective CD4 T cell tolerance to self and to recognition of foreign antigens. Antigen uptake and processing pathways as well as expression of the peptide exchange factors HLA-DM and HLA-DO differ among the various professional and non-professional...
journal_title:Immunogenetics
pub_type: 杂志文章,评审
doi:10.1007/s00251-018-1095-x
更新日期:2019-03-01 00:00:00
abstract::IgM and IgD genes of the Japanese flounder were cloned and characterized from a genomic bacterial artificial chromosome (BAC) library. The micro gene contained four constant region exons (Cmi1-Cmi4), and two transmembrane exons (miTM1 and miTM2), which conforms to the organizational pattern of all other vertebrate mic...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0689-7
更新日期:2004-07-01 00:00:00
abstract::The CCL4 chemokine is secreted by a variety of cells following stimulation. CCL4 affects several different types of cells that are important for acute inflammatory responses and are critical for the development of specific immune responses to foreign antigens. The human genome contains two genes for the CCL4 chemokine...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-003-0636-z
更新日期:2004-01-01 00:00:00
abstract::Assembly of HLA class I molecules was studied using pulse-chase labeling of B-lymphoblastoid cell lines with 35S-methionine, immunoprecipitation with antibodies detecting free or beta 2-microglobulin-associated heavy chain and isoelectric focusing. Marked differences between the products of different class I alleles w...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00216831
更新日期:1993-01-01 00:00:00
abstract::The major histocompatibility complex (MHC) is critical to host-pathogen interactions. Class II MHC is a heterodimer, with α and β subunits encoded by different genes. The peptide-binding groove is formed by the first domain of both subunits (α1 and β1), but studies of class II variation or natural selection focus prim...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-019-01130-z
更新日期:2019-09-01 00:00:00
abstract::Loss of major histocompatibility complex class II (MHCII) antigen expression on diffuse large B cell lymphoma (DLBCL) corresponds closely with significant decreases in patient survival. However, the mechanisms accounting for MHCII loss in DLBCL have not been thoroughly characterized to date. In this report, we demonst...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-009-0418-3
更新日期:2010-02-01 00:00:00
abstract::The genes of the major histocompatibility complex (MHC) are amongst the most variable in vertebrates and represent some of the best candidates to study processes of adaptive evolution. However, despite the number of studies available, most of the information on the structure and function of these genes come from studi...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0456-x
更新日期:2010-08-01 00:00:00
abstract::Class I-like molecules have been immunoprecipitated from Xenopus leukocytes and erythrocytes with alloantisera directed against major histocompatibility complex (MHC)-linked antigens. The heavy chains, depending on the allele examined, have molecular weights of 40 000-44 000 of which 3000 daltons are asparagine-linked...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00345617
更新日期:1984-01-01 00:00:00
abstract::As the only native insular Newfoundland canid between the extinction of the wolf in the 1930s and the recent arrival of coyotes, the red fox (Vulpes vulpes deletrix Bangs 1898) poses interesting questions about genetic distinctiveness and the post-glacial colonization history of the island's depauperate mammalian faun...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-016-0907-0
更新日期:2016-05-01 00:00:00
abstract::Genes of the major histocompatibility complex (MHC) have been characterized in all extant lineages of mammals. The tammar wallaby (Macropus eugenii) is well established as a model marsupial species; however, no classical MHC sequences have been described from this species. We have isolated two MHC class II beta-chain ...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0644-7
更新日期:2004-02-01 00:00:00
abstract::The aim of this study was to evaluate the intra- and inter-population variability of the Gm/Km system in the Madonie Mountains, one of the main geographical barriers in north-central Sicily. We analysed 392 samples: 145 from Alia, 128 from Valledolmo, 25 from Cerda and 94 from Palermo. Serum samples were tested for G1...
journal_title:Immunogenetics
pub_type: 历史文章,杂志文章
doi:10.1007/s00251-003-0628-z
更新日期:2004-01-01 00:00:00
abstract::To identify novel mechanisms regulating allogeneic hematopoietic cell engraftment, we used forward genetics and previously described identification, in mice, of a bone marrow (BM) engraftment quantitative trait locus (QTL), termed Bmgr5. This QTL confers dominant and large allele effects for engraftment susceptibility...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-013-0709-6
更新日期:2013-08-01 00:00:00
abstract::The rhesus macaque ( Macaca mulatta) has become a popular animal model for several human infectious diseases, such as HIV (modeled by SIV infection), hepatitis, and malaria. Investigation of T-cell responses in experimental infectious diseases in rhesus macaques has benefited from an expanding understanding of the div...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-004-0649-2
更新日期:2004-03-01 00:00:00
abstract::Development of an accurate and efficient molecular-based equine MHC class I typing method would facilitate the study of T lymphocyte immune responses in horses. Here, a DNA microarray was designed to detect expressed classical MHC class I genes comprising serologically defined equine leukocyte antigen (ELA)-A haplotyp...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/s00251-010-0463-y
更新日期:2010-09-01 00:00:00
abstract::The susceptibility to cell-mediated cytolysis of cells of the recently developed C57BL/Ka(H-2 ( b )) lymphoma cell line, BL/VL(3), was investigated in allogeneic assays with thymus-dependent lymphocytes (T cells). Compared to EL4, the widely used C57BL/6(H-2 ( b )) lymphoma cell line, BL/VL(3) cells were found to be i...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF01843991
更新日期:1978-12-01 00:00:00
abstract::The identification of 19 different HLA-DPB1 sequences implicates the existence of more DP specificities than can be typed for with cellular methods. How many of the DP beta sequences can be specifically recognized by T cells, and which of the polymorphic regions can contribute to the specificity of allorecognition, is...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00212307
更新日期:1991-01-01 00:00:00
abstract::To analyze how many D-region-encoded molecules could be detected in H-2q, we produced a panel of nine monoclonal antibodies from AKR (KkDk) anti-AKR.M (KkDq) immunizations. All of the Dq region antibodies cross-reacted on Dd and/or Ld, and all except one cross-reacted on Db, confirming the previously observed serologi...
journal_title:Immunogenetics
pub_type: 杂志文章
doi:10.1007/BF00768827
更新日期:1987-01-01 00:00:00