Abstract：
:Binding of peptides to major histocompatibility complex (MHC) molecules is the single most selective step in the recognition of pathogens by the cellular immune system. The human MHC genomic region (called HLA) is extremely polymorphic comprising several thousand alleles, each encoding a distinct MHC molecule. The potentially unique specificity of the majority of HLA alleles that have been identified to date remains uncharacterized. Likewise, only a limited number of chimpanzee and rhesus macaque MHC class I molecules have been characterized experimentally. Here, we present NetMHCpan-2.0, a method that generates quantitative predictions of the affinity of any peptide-MHC class I interaction. NetMHCpan-2.0 has been trained on the hitherto largest set of quantitative MHC binding data available, covering HLA-A and HLA-B, as well as chimpanzee, rhesus macaque, gorilla, and mouse MHC class I molecules. We show that the NetMHCpan-2.0 method can accurately predict binding to uncharacterized HLA molecules, including HLA-C and HLA-G. Moreover, NetMHCpan-2.0 is demonstrated to accurately predict peptide binding to chimpanzee and macaque MHC class I molecules. The power of NetMHCpan-2.0 to guide immunologists in interpreting cellular immune responses in large out-bred populations is demonstrated. Further, we used NetMHCpan-2.0 to predict potential binding peptides for the pig MHC class I molecule SLA-1*0401. Ninety-three percent of the predicted peptides were demonstrated to bind stronger than 500 nM. The high performance of NetMHCpan-2.0 for non-human primates documents the method's ability to provide broad allelic coverage also beyond human MHC molecules. The method is available at http://www.cbs.dtu.dk/services/NetMHCpan.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Hoof I,Peters B,Sidney J,Pedersen LE,Sette A,Lund O,Buus S,Nielsen Mdoi
10.1007/s00251-008-0341-zsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
1-13issue
1eissn
0093-7711issn
1432-1211journal_volume
61pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract：:We reported previously that human gene MAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with a MAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12 MAGE ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01246677
更新日期：1994-01-01 00:00:00
abstract：:The factors which influence expression of autoimmune disease in the central nervous system are still poorly understood. We determined the characteristics of experimental autoimmune encephalomyelitis (EAE) in twelve different inbred strains of mice using either mouse spinal cord homogenate or synthetic peptides as the ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF02602559
更新日期：1996-01-01 00:00:00
abstract：:Natural killer (NK) cells express receptors for MHC class I that contain immunoreceptor tyrosine-based inhibitory motif (ITIM) sequences in their cytoplasmic domain. Whereas these receptors inhibit NK cell cytotoxicity, certain isoforms of these NK receptors (e.g., KIR2DS, CD94/NKG2C, and Ly49D) do not have ITIMs, but...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510050642
更新日期：2000-05-01 00:00:00
abstract：:In mammals many natural killer (NK) cell receptors, encoded by the leukocyte receptor complex (LRC), regulate the cytotoxic activity of NK cells and provide protection against virus-infected and tumor cells. To investigate the origin of the Ig-like domains encoded by the LRC genes, a subset of C2-type Ig-like domain s...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-004-0764-0
更新日期：2005-04-01 00:00:00
abstract：:A cDNA library screening using the conserved exon 4 of Atlantic salmon Mhc class I as probe provided the basis for a study on Mhc class I polymorphism in a breeding population. Twelve different alleles were identified in the 82 dams and sires studied. No individual expressed more than two alleles, which corresponded t...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-002-0499-8
更新日期：2002-11-01 00:00:00
abstract：:Using the fluorescence-activated cell sorter (FACS II), we have analyzed the expression of H-2K- and H-2D-gene products on the membrane of various cellular components of the murine immune system. Using this serological technique we show a basic difference between T and B lymphocytes. Whereas all cellular components an...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01567827
更新日期：1980-01-01 00:00:00
abstract：:Pigtail macaques (Macaca nemestrina) are an increasingly common primate model for the study of human AIDS. Major Histocompatibility complex (MHC) class I-restricted CD8(+) T cell responses are a critical part of the adaptive immune response to HIV-1 in humans and simian immunodeficiency virus (SIV) in macaques; howeve...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-006-0164-8
更新日期：2006-12-01 00:00:00
abstract：:The glycosyl phosphatidylinositol (GPI)-linked antigen recognized by monoclonal antibody (mAb) MEM-102 is expressed on all peripheral blood lymphocytes, both resting and activated. Its properties are very similar to a previously described activation antigen, Blast-1. The amino acid sequence deduced from the structure ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00210823
更新日期：1991-01-01 00:00:00
abstract：:Genes encoding the horse (Equus caballus) T-cell receptor beta chain (TCRB) were cloned and characterized. Of 33 cDNA clones isolated from the mesenteric lymph node, 30 had functionally rearranged gene segments, and three contained germline sequences. Sixteen unique variable segments (TCRBV), 14 joining genes (TCRBJ),...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00188177
更新日期：1994-01-01 00:00:00
abstract：:Mammalian interleukin-4 (IL-4) and IL-13 are T helper type 2 (Th2) cytokines with pleiotropic functions in immunity. They signal through receptors containing IL-4Rα and IL-2Rγ or IL-13Rα1. In addition, a decoy receptor, IL-13Rα2, is known to exist and modulates the function of IL-13. The existence of fish orthologues ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-010-0508-2
更新日期：2011-04-01 00:00:00
abstract：:Described here is a new, more efficient method for defining major histocompatibility complex-Y (MHC-Y) genotypes in chickens. The MHC-Y region is genetically independent from the classical MHC (MHC-B) region. MHC-Y is highly polymorphic and potentially important in the genetics of disease resistance. MHC-Y haplotypes ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-020-01166-6
更新日期：2020-07-01 00:00:00
abstract：:In the mouse three lymphocyte gene families have been positioned on the proximal region of chromosome 6. Originally the immunoglobulin kappa light chain (Igk) and the thymocyte surface antigens Lyt-2 and Lyt-3 were assigned to chromosome 6, and recently the beta chain of the T-cell receptor for antigen was positioned ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00404487
更新日期：1985-01-01 00:00:00
abstract：:A DBA/1 B10.D2-specific monoclonal antibody (CY34) is described which defines a new murine B lymphocyte differentiation antigen designated Lyb-8.2. The ontogeny, strain distribution, and cell-surface density of the antigen were studied by radioimmunoassay and by fluorescence-activated cell sorter (FACS) analysis. Lyb-...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00372098
更新日期：1982-01-01 00:00:00
abstract：:Genes of the major histocompatibility complex (MHC) have been characterized in all extant lineages of mammals. The tammar wallaby (Macropus eugenii) is well established as a model marsupial species; however, no classical MHC sequences have been described from this species. We have isolated two MHC class II beta-chain ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-004-0644-7
更新日期：2004-02-01 00:00:00
abstract：:The protein kinase, interferon-inducible double-stranded (ds)RNA-dependent activator (PRKRA) is a dsRNA-binding protein which activates a protein kinase participating in the antiviral activity of interferon. Our previous studies indicated that the nucleotide sequence encoding PRKRA, which appeared to be an intronless ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510000270
更新日期：2001-01-01 00:00:00
abstract：:Transcription of major histocompatibility complex class I genes is controlled by the class I regulatory complex in the 5' flanking region. To investigate the molecular basis of this region, we studied the polymorphism of the promoter of the HLA-B locus extending from the ATG transcription initiation signal to -284 bas...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00163991
更新日期：1995-01-01 00:00:00
abstract：:We examined the expression of H-Y antigen in 14 XO female mice using three monoclonal H-Y-specific antibodies. We found that spleen and liver cells from XO mice removed the reactivity of these antibodies at the same efficacy as XY cells. However radiobinding assays on cultured XO cells suggested a qualitative or quant...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00401354
更新日期：1983-01-01 00:00:00
abstract：:The common fruit fly Drosophila melanogaster is a powerful model for studying signaling pathway regulation. Conserved signaling pathways underlying physiological processes signify evolutionary relationship between organisms and the nature of the mechanisms they control. This study explores the cross-talk between the w...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-019-01119-8
更新日期：2019-07-01 00:00:00
abstract：:The only avian major histocompatibility complex (Mhc) genes thus far identified are from species of the relatively small order of Galliformes, while by far the largest order of Passeriformes (songbirds), containing some 60% of extant bird species, has not been studied at all in this regard. The Galliformes emerged mor...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00176443
更新日期：1995-01-01 00:00:00
abstract：:We have performed an extensive analysis of Th1/Th2 cytokine receptors IL2Ralpha, IL4Ralpha, IL10Ralpha, and IFNgammaR1 gene polymorphisms to evaluate their impact on AIDS progression. The coding regions and promoters of these genes were sequenced in the genetics of resistance to immunodeficiency virus cohort, composed...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-005-0072-3
更新日期：2006-04-01 00:00:00
abstract：:Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401 (DRw14-Dw9) and DRB1*1402 (DRw14-Dw16). The second exon of the DRB1 gene of the novel DRw14 allele (designated DRB1-14c) ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00211645
更新日期：1990-01-01 00:00:00
abstract：:The anti-HLA-DQ3 monoclonal antibodies (mAb) KS13, SO1, SO2, SO3, SO4, and SO5 recognize spatially close but distinct antigenic determinants, since they crossinhibit each other in their binding to HLA-DQ3 antigens, but do not share idiotopes recognized in their antigen combining site by syngeneic and anti-id antisera ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00178583
更新日期：1995-01-01 00:00:00
abstract：:The interaction between 9-mer peptides and HLA-B51 molecules was investigated by quantitative peptide binding assay using RMA-S cells expressing human beta2-microglobulin and HLA-B51 molecules. Of 147 chemically synthesized 9-mer peptides possessing two anchor residues corresponding to the motif of HLA-B*5101 binding ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF02440994
更新日期：1996-01-01 00:00:00
abstract：:Celiac disease is a chronic inflammatory condition of the small intestine caused by aberrant adaptive immune response to gluten protein from wheat and related cereal plants. Over 90% of celiac disease patients carry the HLA-DQ2.5 allotype and HLA-DQ2.5 presents gluten peptides to gluten-reactive CD4+ T cells in celiac...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-019-01138-5
更新日期：2019-11-01 00:00:00
abstract：:In the absence of suitable rodent animal models for Plasmodium falciparum malaria, the efficacy testing of asexual blood-stage vaccine candidates in Aotus nancymaae represents a tool to select between different formulations before conducting expensive human clinical trials. CpG oligonucleotides (ODN) specifically prom...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-005-0789-z
更新日期：2005-05-01 00:00:00
abstract：:The HLA-A2 antigen expressed by donor OZB can be distinguished from the main HLA-A2.1 subtype by isoelectric focusing - it is one charge unit more acidic - and by some alloreactive T-cell clones but not by cytolytic T lymphocyte lines. The structure of variant OZB has been examined by comparative peptide mapping with ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00395130
更新日期：1988-01-01 00:00:00
abstract：:The polymorphism of exon 2 of the DAB genes (major histocompatibility complex [MHC] class IIB) was investigated for the first time in the freshwater cyprinid fish species, Squalius cephalus, in the wide range of its distribution in Europe. We identified 111 different MHC class IIB variants in 15 chub populations distr...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-010-0495-3
更新日期：2011-03-01 00:00:00
abstract：:UV-inactivated GCHV (grass carp hemorrhage virus) is able to induce an antiviral state in cultured CAB cells (crucian carp Carassius auratus blastulae embryonic cells) via the production of interferon (IFN). In the current work, the full-length cDNAs of two Mx genes, termed CaMx1 and CaMx2, have been cloned and sequen...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-004-0658-1
更新日期：2004-04-01 00:00:00
abstract：:The sheep immunoglobulin heavy chain Igh-J locus has been characterized in order to determine the genomic organization of JH segments and their contribution to heavy chain diversity. The locus contains six segments, of which two are functional and four are apparently pseudogenes. These segments span a 1.8 kilobase (kb...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510050274
更新日期：1997-01-01 00:00:00
abstract：:Immunofluorescent and immunoelectron-microscopic staining methods were utilized to investigate the localization of Ia antigens in murine keratinizing epithelia. Approximately 3-5% of epidermal cells were shown to be Ia positive. Only dendritic Langerhans cells in the interfollicular epidermis and outer root sheaths we...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01844036
更新日期：1978-12-01 00:00:00
