Abstract:
:A large number of HLA-Cw4 (Cw *0402) peptides were purified, sequenced, and identified from breast and ovarian carcinoma cell lines. HLA-Cw4 molecules were expressed in these cells as soluble, secreted HLA (sHLA) and recovered from the growth medium. The peptides were separated by capillary reversed-phase HPLC and analyzed by tandem mass-spectrometry. The resulting peptides fit to some extent, but not completely, the known consensus of the Cw4 peptide-binding motif. Among the identified peptides, there are a few that originate from proteins of possible interest for cancer immunotherapy or diagnostics, including mucin-5B, ART-1, fatty acid synthase, putative prostate cancer tumor suppressor, DNA topoisomerase-1, and Rac1. This work demonstrates that large-scale identification of HLA peptides recovered from sHLA is an advantageous approach for establishing the HLA peptide consensus of different haplotypes and the identification of useful peptides for treatment of diseases such as cancer, viral, and autoimmune diseases.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Buchsbaum S,Barnea E,Dassau L,Beer I,Milner E,Admon Adoi
10.1007/s00251-003-0570-0subject
Has Abstractpub_date
2003-06-01 00:00:00pages
172-176issue
3eissn
0093-7711issn
1432-1211pii
10.1007/s00251-003-0570-0journal_volume
55pub_type
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