Abstract：
:There have been several attempts over the years to identify positions in the peptide-binding region (PBR) of human leukocyte antigens (HLA) that influence the specificity of bound amino acids (AAs) at each position in the peptide. Originally, six pockets (A-F) were defined by calculating the surface area of the PBR on the crystal structure of HLA-A2 molecules. More recent crystallographic analyses of a variety of HLA alleles have led to broader pocket definitions. In this study, we examined the peptide-binding specificity of HLA-B*41 alleles and compared our results with the available pocket definitions. By generating recombinant HLA-B molecules and studying the eluted peptides by mass spectrometry and pool sequencing, we detected two different POmega peptide motifs within the B*41 group: Leu vs Val/Pro. Specificity was dependent on the presence of Leu (B*4102, B*4103, and B*4104) vs Trp (B*4101, B*4105, and B*4106) at AA position 95 in the HLA molecule, whose impact on POmega has been a subject of controversy in current pocket definitions. In contrast, the Arg97Ser mutation did not affect pocket F binding specificity in B*41 subtypes although residue 97 was previously identified as a modulator of peptide binding for several HLA class I alleles. According to most pocket definitions, this study shows that the Asn80Lys substitution in B*4105 impels the peptide's POmega anchor toward more promiscuity. Our sequencing results of peptides eluted from HLA-B*41 variants demonstrate the limitations of current pocket definitions and underline the need for an extended peptide motif database for improved understanding of peptide-major histocompatibility complex interactions.
journal_name
Immunogeneticsjournal_title
Immunogeneticsauthors
Bade-Doeding C,DeLuca DS,Seltsam A,Blasczyk R,Eiz-Vesper Bdoi
10.1007/s00251-007-0197-7subject
Has Abstractpub_date
2007-04-01 00:00:00pages
253-9issue
4eissn
0093-7711issn
1432-1211journal_volume
59pub_type
杂志文章相关文献
IMMUNOGENETICS文献大全abstract：:The variability of immune responses modulated by human leukocyte antigen (HLA) genes and secreted cytokines is a significant factor in the development of a protective effect of measles vaccine. We studied the association between type 1 helper T cells (Th1)- and Th2-like cytokine immune responses and HLA class I allele...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-005-0061-6
更新日期：2005-12-01 00:00:00
abstract：:The inbred strain STS/A exhibits a higher proliferative response in the mixed lymphocyte culture (MLC) to stimulator cells of all 11 tested inbred mouse strains with 10 different major histocompatibility complex (MHC) haplotypes, as well as to stimulation with IL-2 than does the strain BALB/cHeA. However, alloantigen-...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF02602654
更新日期：1996-01-01 00:00:00
abstract：:Many immunoglobulin variable region (IgV) genes are present in the vertebrate genome and provide a basis for antibody diversity. IgV genes have been classified into distinct families according to DNA sequence similarity. Comparisons of VH and VL genes from two mammalian species (mouse and human) have led to the conclu...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00188428
更新日期：1995-01-01 00:00:00
abstract：:Four cDNA probes for the human major histocompatibility complex (MHC) were used to investigate the sheep MHC, in conjunction with serological typing for ovine lymphocyte antigen (OLA). Lymphocytes from a family (two parents and five offspring) of Romanov sheep were subjected to genomic DNA digestion by the restriction...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00430918
更新日期：1985-01-01 00:00:00
abstract：:Natural killer (NK) cells express receptors for MHC class I that contain immunoreceptor tyrosine-based inhibitory motif (ITIM) sequences in their cytoplasmic domain. Whereas these receptors inhibit NK cell cytotoxicity, certain isoforms of these NK receptors (e.g., KIR2DS, CD94/NKG2C, and Ly49D) do not have ITIMs, but...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510050642
更新日期：2000-05-01 00:00:00
abstract：:UV-inactivated GCHV (grass carp hemorrhage virus) is able to induce an antiviral state in cultured CAB cells (crucian carp Carassius auratus blastulae embryonic cells) via the production of interferon (IFN). In the current work, the full-length cDNAs of two Mx genes, termed CaMx1 and CaMx2, have been cloned and sequen...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-004-0658-1
更新日期：2004-04-01 00:00:00
abstract：:The N-terminal domain of the chemokine CCL5/regulated upon activation normal T cell expressed and secreted (RANTES) has been shown to be critical for its biological activity on leukocytes. Several N-terminus-modified CCL5/RANTES derivatives, such as N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (A...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-006-0133-2
更新日期：2006-07-01 00:00:00
abstract：:Immunofluorescent and immunoelectron-microscopic staining methods were utilized to investigate the localization of Ia antigens in murine keratinizing epithelia. Approximately 3-5% of epidermal cells were shown to be Ia positive. Only dendritic Langerhans cells in the interfollicular epidermis and outer root sheaths we...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01844036
更新日期：1978-12-01 00:00:00
abstract：:Human CS1, also known as novel Ly9, 19A24, or CRACC, is a member of the immunoglobulin gene superfamily (IgSF) expressed on natural killer cells and other leukocytes. Here we describe the cloning of the mouse homologue of this gene. The mouse novel Ly9 gene is shown to encode a transmembrane protein composed of two ex...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-002-0483-3
更新日期：2002-09-01 00:00:00
abstract：:The cattle major histocompatibility complex (MHC) region contains a variable number of classical class I genes encoding polymorphic molecules involved in antigen presentation. Six classical class I genes have been described, but assigning sequences to these genes has proved problematic. We propose a refinement of the ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-012-0611-7
更新日期：2012-06-01 00:00:00
abstract：:An analysis of mRNA structures as deduced from the amino acid sequences of immunoglobulin heavy chains reveals possible base-paired regions within the sequences coding for the 20 C-terminal amino acids of the human μ and α chains. The two regions are similar in structure and contain a palindrome which might serve as a...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01843981
更新日期：1978-12-01 00:00:00
abstract：:Assembly of HLA class I molecules was studied using pulse-chase labeling of B-lymphoblastoid cell lines with 35S-methionine, immunoprecipitation with antibodies detecting free or beta 2-microglobulin-associated heavy chain and isoelectric focusing. Marked differences between the products of different class I alleles w...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00216831
更新日期：1993-01-01 00:00:00
abstract：:We have recently mapped the human FCGR2 gene to chromosome 1 bands q23-q24. In situ hybridization of FCGR2 cDNA with a cell line containing a t(1:19)(q23;p13) derived from a patient with pre-B ALL has allowed a more accurate localization of this gene to chromosome 1 band q23. Furthermore, this study indicated a splitt...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF02115010
更新日期：1990-01-01 00:00:00
abstract：:Apoptosis has been suggested as a major mechanism for the CD4(+) T-lymphocyte depletion observed in patients infected with human immunodeficiency virus 1 (HIV-1). To evaluate the impact of genetic variations to apoptosis during progression of acquired immunodeficiency syndrome (AIDS), we have performed an extensive ge...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-004-0664-3
更新日期：2004-04-01 00:00:00
abstract：:Eleven new C4d genomic primate sequences of the fourth complement factor (C4) have been obtained. Seven of them belong to five species not yet explored for this gene: Pan paniscus (pygmy chimpanzee), Cercopithecus aethiops (green monkey), Macaca mulatta (rhesus monkey), Macaca fascicularis (cynomolgus), and Saguinus o...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00177822
更新日期：1994-01-01 00:00:00
abstract：:Ranatuerins are antimicrobial peptides of the innate immune system found in ranid frogs. We previously presented evidence that a positive selective sweep had fixed a single allele at the Ranatuerin2 locus in the northern leopard frog (Rana pipiens). In this paper, we further investigate the evolutionary history of ran...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-010-0430-7
更新日期：2010-05-01 00:00:00
abstract：:The effect of the H-2dm1 mutation on Qa-m2 expression was examined. The mutant strain B10.D2-H-2dm1 showed a fourfold increase in Qa-m2 expression when compared with the parental strain B10.D2. Qa-m2 molecules immunoprecipitated from B10.D2-H-2dm1, C57BL/10, and B10.D2 spleen cells were identical by two-dimensional (2...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00345969
更新日期：1983-01-01 00:00:00
abstract：:Dendritic cells (DC) are specialist antigen presenting cells which capture antigens in the periphery, migrate centrally, and present the processed antigens in the context of major histocompatibility complex and appropriate co-stimulatory molecules to T lymphocytes for the initiation of an immune response. DEC-205 has ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510050381
更新日期：1998-05-01 00:00:00
abstract：:Human and mouse major histocompatibility complex class II beta chain alleles associated with predisposition to type I diabetes often encode a non-charged residue at position 57 rather than the negatively charged aspartate residue characteristic of non-susceptible haplotypes. The mechanism(s) whereby this polymorphism ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510050351
更新日期：1998-01-01 00:00:00
abstract：:Using mouse bone marrow-derived macrophages we determined the role of interferon (IFN)-gamma at the different steps in expression of the I-A alpha chain of MHC class II molecules, from transcription to the cell surface. Levels of transcription, RNA, and protein were low in cells not stimulated with IFN-gamma. Treatmen...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s002510100312
更新日期：2001-03-01 00:00:00
abstract：:Mouse leukosialin, previously known as the 3E8 antigen, is expressed primarily on cells of the hematopoietic and lymphoid lineages and is shown to be the mouse homologue to the human leukosialin/sialophorin and rat W3/13 molecules. A partial leukosialin cDNA clone was isolated via cross-species hybridization with a po...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF02115004
更新日期：1990-01-01 00:00:00
abstract：:We reported previously that human gene MAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with a MAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12 MAGE ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF01246677
更新日期：1994-01-01 00:00:00
abstract：:The A15, L6, and S5.7(TAPA1) proteins are members of the transmembrane 4 superfamily (TM4SF). The A15 is expressed in immature human T cells and in the human brain. The MXS1(CCG-B7) gene which codes for A15 contains triplet nucleotide repeats which have been associated with neuropsychiatric diseases such as Huntington...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00189229
更新日期：1994-01-01 00:00:00
abstract：:MICA and MICB genes encode MHC class I chain-related proteins, which are polymorphic, do not appear to present peptides or associate with beta(2)-microglobulin, and are expressed predominantly in epithelial cells, endothelial cells, fibroblasts and several cultured cell lines. Alternatively spliced isoforms are known ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-002-0496-y
更新日期：2002-12-01 00:00:00
abstract：:The tripartite motif protein (TRIM)5α/CypA fusion protein TRIMCyp in Old World monkeys is generally considered unable to restrict HIV-1 replication. Monkeys with TRIMCyp can serve as a unique animal model for studies of HIV-1 infection. The present study investigated the distribution and expression status of TRIMCyp i...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-012-0670-9
更新日期：2013-03-01 00:00:00
abstract：:To describe the polymorphism of the DRA gene in Macaca fascicularis, we have studied 141 animals either at cDNA level (78 animals from Mauritius, the Philippines, and Vietnam) or genomic level (63 animals from the Philippines, Indonesia, and Vietnam). In total, we characterized 22 cDNA DRA alleles, 13 of which had not...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-009-0421-8
更新日期：2010-03-01 00:00:00
abstract：:Many fishes express high levels of intraspecific variability, often linked to resource partitioning. Several studies show that a species' evolutionary trajectory of adaptive divergence can undergo reversals caused by changes in its environment. Such a reversal in neutral genetic and morphological variation among lake ...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-017-0996-4
更新日期：2018-01-01 00:00:00
abstract：:Ataxia-telangiectasia (A-T), a rare inherited disorder, usually affects the nervous and immune systems, and occasionally other organs. A-T is associated mainly with mutations in the ataxia telangiectasia mutated (ATM) gene, which encodes a protein kinase that has a major role in the cellular response to DNA damage. We...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-017-0983-9
更新日期：2017-07-01 00:00:00
abstract：:Kinetics and family transmission of antigen-specific in vitro cell-mediated responses were investigated in 68, and serum-antibody responses to tetanus toxoid (TT) in 73 individuals from a total of 12 families. Proliferative responses to highly purified TT monomer were studied in 6- to 7-day lymphocyte cultures. The ef...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/BF00372467
更新日期：1983-01-01 00:00:00
abstract：:The KIR gene cluster exhibits a high degree of polymorphism in terms of gene content as well as allelic polymorphism, and data suggest that it is evolving rapidly. The KIR3DL1 locus is one of the most polymorphic loci within this cluster and is unique in that it encodes an activating receptor KIR3DS1, as well as multi...
journal_title：Immunogenetics
pub_type： 杂志文章
doi：10.1007/s00251-007-0240-8
更新日期：2007-10-01 00:00:00
