A modified method for the estimation of galactose-1-phosphate.

abstract：:An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...

journal_title：Annals of clinical biochemistry

authors： Goldfinch ME,Maguire GA

更新日期：1988-01-01 00:00:00

abstract：:Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-...

journal_title：Annals of clinical biochemistry

authors： Yoshimoto S,Hirota M,Ohboshi C,Shima K

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:Supported liquid extraction (SLE) techniques are relatively new compared to other sample preparation approaches such as solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PPE). We investigated the use of SLE as an alternative to SPE for the liquid chromatography tandem ma...

journal_title：Annals of clinical biochemistry

authors： Owen LJ,Keevil BG

更新日期：2013-09-01 00:00:00

abstract：:A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...

journal_title：Annals of clinical biochemistry

authors： Stewart MJ,Willis RG

更新日期：1975-01-01 00:00:00

abstract：BACKGROUND:The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the ...

journal_title：Annals of clinical biochemistry

authors： McEneny J,McPherson PA,McGinty A,Hull SS,McCance DR,Young IS

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND:Thiopurine methyltransferase (TPMT) catalyses the S-methylation of 6-thiopurine drugs, which are commonly used in the treatment of autoimmune diseases, leukaemia and organ transplantation. TPMT activity is polymorphic as a result of gene mutations. Ethnic variations in phenotype and genotype have been identi...

journal_title：Annals of clinical biochemistry

authors： Laróvere LE,de Kremer RD,Lambooy LH,De Abreu RA

更新日期：2003-07-01 00:00:00

abstract：:Three sensitive assays (Behring, Organon and LKB-Pharmacia) for measurement of serum thyrotropin (TSH) were evaluated. All three assays showed good precision, sensitivity, linearity and for practical purposes negligible high dose hook effects. The correlations between the assays were excellent, but due to standardisat...

journal_title：Annals of clinical biochemistry

authors： Bonte HA,Vermes I,v d Sluijs Veer G

更新日期：1989-11-01 00:00:00

abstract：:Requirements for information technology in pathology now extend well beyond the provision of purely analytical data. With the aim of achieving seamless integration of laboratory data into the total clinical pathway, "informatics"--the art and science of turning data into useful information--is becoming increasingly im...

journal_title：Annals of clinical biochemistry

authors： Jones R,O'Connor J

更新日期：2004-05-01 00:00:00

abstract：:During incubation with rabbit blood in vitro rabbit-muscle lactate dehydrogenase-5 was inactivated at a rate similar to that observed in vivo. By contrast plasma and plasma containing erythrocytes had no effect on the enzyme activity, but plasma containing leucocytes inactivated the enzyme at the same rate as whole bl...

journal_title：Annals of clinical biochemistry

authors： Qureshi AR,Wilkinson JH

更新日期：1977-01-01 00:00:00

abstract：:Most thyroid-stimulating hormone (TSH) assays now have the sensitivity required by thyroid guidelines and allow the reliable identification of patients with both overt and subclinical hyperthyroidism. Clinical guidelines usually quote decision limits for TSH, but often ignore the issue of whether variability in bias b...

journal_title：Annals of clinical biochemistry

authors： Beckett G,MacKenzie F

更新日期：2007-05-01 00:00:00

abstract：:We evaluated a novel assay for the measurement of 1,25 dihydroxyvitamin D (1,25 (OH)2D). Immunoextraction of 1,25(OH)2D is performed using a mini column containing a solid-phase monoclonal antibody followed by radioimmunoassay (RIA) using an 125I-labelled 1,25(OH)2D derivative tracer and Sac-cell separation. The mean ...

journal_title：Annals of clinical biochemistry

authors： Fraser WD,Durham BH,Berry JL,Mawer EB

更新日期：1997-11-01 00:00:00

abstract：:A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...

journal_title：Annals of clinical biochemistry

authors： Willemsen JJ,Ross HA,Wolthers BG,Sweep CG,Kema IP

更新日期：2001-11-01 00:00:00

abstract：:We present two cases of spuriously high cerebrospinal fluid glucose concentration with approximately normal blood glucose concentrations. The cause of these abnormal findings was the use of inappropriate collection tubes during the pre-analytical phase. Whilst no patient harm was identified following this error, signi...

journal_title：Annals of clinical biochemistry

authors： Broz P,Rajdl D,Racek J,Petr T,Vitek L

更新日期：2015-01-01 00:00:00

abstract：:The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performanc...

journal_title：Annals of clinical biochemistry

authors： Bonham JR,Downing M,Pollitt RJ,Manning NJ,Carpenter KH,Olpin SE,Allen JC,Worthy E

更新日期：1994-03-01 00:00:00

abstract：:Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsifie...

journal_title：Annals of clinical biochemistry

authors： Yamaguchi T,Murano T,Tatsuno I,Hiruta N,Suzuki T,Sawada S,Katagiri H,Shirai K,Schneider WJ,Bujo H

更新日期：2017-11-01 00:00:00

abstract：:The effect on the concentrations of specific serum proteins and tumour markers, of heat-treating samples at 56 degrees C for 30 min to inactivate HIV, was investigated. Statistically significant decreases, that may affect clinical decisions, were observed for alpha-1-antitrypsin, beta-2-microglobulin, IgE, fibrinogen,...

journal_title：Annals of clinical biochemistry

authors： Gleeson M,Herd L,Burns C

更新日期：1990-11-01 00:00:00

abstract：BACKGROUND:The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples. METHODS:Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium ED...

journal_title：Annals of clinical biochemistry

authors： English E,McFarlane I,Taylor KP,Halsall DJ

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Ascorbic acid can interfere with methodologies involving redox reactions, while comprehensive studies on main chemistry analysers have not been reported. We therefore attempted to determine the interference of ascorbic acid with analytes on the Beckman Synchron LX20. METHODS:Various concentrations of ascorb...

journal_title：Annals of clinical biochemistry

authors： Meng QH,Irwin WC,Fesser J,Massey KL

更新日期：2005-11-01 00:00:00

abstract：:We have studied 46 patients, 30 men and 16 women, with type 2 (non-insulin-dependent) diabetes in a follow-up period of 6-52 months (mean 30 months). The patients were consecutively entered in the study from the out-patient diabetic clinic. None had urinary tract infections nor proteinuria at entry. Investigations wer...

journal_title：Annals of clinical biochemistry

authors： Friis T,Pedersen LR

更新日期：1997-05-01 00:00:00

abstract：BACKGROUND:Glycated haemoglobin (HbA1c) concentration is lower relative to glyacemic control in diabetic patients on haemodialysis. However, it is unknown as to whether this is also true for diabetic patients with end-stage renal disease but not on haemodialysis. METHODS:Correlations between HbA1c or glycated albumin ...

journal_title：Annals of clinical biochemistry

authors： Shima K,Chujo K,Yamada M,Komatsu M,Noma Y,Mizuguchi T

更新日期：2012-01-01 00:00:00

abstract：:Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...

journal_title：Annals of clinical biochemistry

authors： Ko GT,Chan JC,Yeung VT,Chow CC,Li JK,Lau MS,Mackay IR,Rowley MJ,Zimmet P,Cockram CS

更新日期：1998-11-01 00:00:00

abstract：:Clinical biochemists have long known the analytical and clinical limitations of creatinine and creatinine clearance measurement in the assessment of glomerular filtration rate (GFR). This background is reviewed in the article before assessing the utility of cystatin C, the most promising replacement biochemical marker...

journal_title：Annals of clinical biochemistry

authors： Newman DJ

更新日期：2002-03-01 00:00:00

abstract：BACKGROUND:Measurement of aldosterone and/or renin is essential to aid the differential diagnosis of secondary hypertension, guide strategy for therapeutic management of hypertension and assess adequacy of mineralocorticoid replacement. AIM:The objective was to establish normative data for aldosterone and renin using ...

journal_title：Annals of clinical biochemistry

authors： O'Shea P,Brady JJ,Gallagher N,Dennedy MC,Fitzgibbon M

更新日期：2016-05-01 00:00:00

abstract：:Thyroglobulin (Tg) is a tumour marker for differentiated thyroid cancer. Interpretation requires a knowledge of the current thyrotropin (TSH) concentration as secretion is TSH-dependent. While a raised serum Tg may be indicative of residual or recurrent thyroid cancer, trauma to the thyroid (e.g. surgical, biopsy or d...

journal_title：Annals of clinical biochemistry

authors： Clark P,Franklyn J

更新日期：2012-07-01 00:00:00

abstract：:Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...

journal_title：Annals of clinical biochemistry

authors： Meek CL

更新日期：2017-01-01 00:00:00

abstract：:Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...

journal_title：Annals of clinical biochemistry

authors： Reynolds TM

更新日期：1997-05-01 00:00:00

abstract：:Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error. ...

journal_title：Annals of clinical biochemistry

authors： McLelland AS,Fleck A,Wagstaff D,Robinson R

更新日期：1975-07-01 00:00:00

abstract：BACKGROUND:Placental growth factor (PlGF) and soluble-fms-like tyrosine kinase 1 (sFlt-1) are biomarkers of placental function used to aid the diagnosis and prediction of pregnancy complications. This work verified the analytical performance of both biomarkers and provides preliminary diagnostic accuracy data to identi...

journal_title：Annals of clinical biochemistry

authors： Armstrong-Buisseret LK,Haslam S,James T,Bradshaw L,Heazell AE

更新日期：2020-05-01 00:00:00

abstract：:Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...

journal_title：Annals of clinical biochemistry

authors： Cope G,Nayyar P,Holder R,Brock G,Chapple I

更新日期：2000-09-01 00:00:00

abstract：:Iron status is influenced by inflammation when the normal control of iron metabolism is reorganized by the primary mediators of the acute phase response, tumour necrosis factor-alpha and interleukin-1. The objective of this review is to show how indices of iron status, particularly haemoglobin, serum ferritin and solu...

journal_title：Annals of clinical biochemistry

authors： Northrop-Clewes CA

更新日期：2008-01-01 00:00:00