Abstract:
:We evaluated a novel assay for the measurement of 1,25 dihydroxyvitamin D (1,25 (OH)2D). Immunoextraction of 1,25(OH)2D is performed using a mini column containing a solid-phase monoclonal antibody followed by radioimmunoassay (RIA) using an 125I-labelled 1,25(OH)2D derivative tracer and Sac-cell separation. The mean recovery of 1,25(OH)2D3 was 101%, linearity was excellent, inter- and intra-assay coefficients of variation were 9, 8 and 13% and 11, 10 and 14% at low, medium and high concentrations of 1,25 (OH)2D3, respectively. The cross-reactivity of vitamin D metabolites was < 0.0015% for 25-hydroxyvitamin D3, 24, 25 dihydroxyvitamin D3 and dihydrotachysterol and 0.54% for 1 alpha calcidol. 1,25 dihydroxyvitamin D2 cross-reactivity was 79%. The detection limit of the assay was 5 pmol/L. Comparison with a commercial radio receptor assay (RRA) and an in-house RIA gave regression equations of y = 0.94x + 11.8 (r = 0.98) and y = 0.91x-1.7 (r = 0.95), respectively, with no major discrepancies between the methods in all patient groups studied. Plasma concentrations of 1,25(OH)2D obtained with the assay were as follows: normal, unsupplemented subjects: mean 88, range 48-155 pmol/L, n = 68, patients with chronic renal failure: mean 11, range 3-36 pmol/L, n = 27, primary hyperparathyroidism: mean 198, range 130-299 pmol/L, n = 23, Paget's disease: mean 92, range 42-149 pmol/L, n = 24, osteomalacia: mean 43, range 27-61 pmol/L, n = 9. A minimum sample volume of 300 microL is required, the hands-on time is significantly less than other commercial assays and the measuring procedure is gamma counting rather than scintillation counting. The assay offers several advantages over previous methods and should allow more laboratories to offer measurement of 1,25(OH)2D as part of their repertoire.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Fraser WD,Durham BH,Berry JL,Mawer EBdoi
10.1177/000456329703400606subject
Has Abstractpub_date
1997-11-01 00:00:00pages
632-7eissn
0004-5632issn
1758-1001journal_volume
34 ( Pt 6)pub_type
临床试验,杂志文章abstract::Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200135
更新日期:1975-07-01 00:00:00
abstract::The immunosuppressive agent mycophenolate mofetil is a prodrug for the active compound mycophenolic acid. Following organ transplantation, it is used mostly in combination with other immunosuppressive drugs, but it has also been used as primary therapy. Although in the pivotal clinical studies the drug was given in fi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021902035
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND AND METHODS:We report a case of insulinoma in which the diagnosis was very challenging as some of the biochemical data were consistently equivocal. In order to assess the relative reliability of the analytical tests, retrospective biochemical data on 45 other cases of histologically confirmed insulinoma were...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303770367315
更新日期:2003-11-01 00:00:00
abstract::Some inherited metabolic disorders (IMDs) can cause significant complications during pregnancy, affecting the mother and/or the fetus. Although it appears that only a minority of IMDs have these effects, experience is still being acquired. For some disorders, patients will not have reached child-bearing age. Pregnanci...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456302320314458
更新日期:2002-09-01 00:00:00
abstract::Background The aim of the present study is to evaluate the utility of extraction versus non-extraction-based commercial melatonin ELISA kits for determining the melatonin concentration in amniotic fluid obtained in early and late pregnancy. Methods Pregnancy duration less than 28 weeks was defined as early and from 28...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563216645123
更新日期:2017-01-01 00:00:00
abstract::A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000105
更新日期:1983-01-01 00:00:00
abstract::Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsifie...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217693258
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND AND AIM:Familial hypercholesterolaemia is caused by variants in the low-density lipoprotein cholesterol metabolic pathway involving LDLR, APOB and PCSK9 genes. A national genetic testing service in Wales, UK has observed that no familial hypercholesterolaemia variant is found in almost 80% patients with the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218793165
更新日期:2019-01-01 00:00:00
abstract::Guaiac-based faecal occult blood tests (gFOBT) are still used in asymptomatic population bowel screening programmes but are being replaced by faecal immunochemical tests (FIT) for haemoglobin. gFOBT have many well-documented disadvantages and there is little evidence for their use in assessment of the symptomatic. Man...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2012.012065
更新日期:2012-11-01 00:00:00
abstract::An audit of troponin measurement protocols in use in Wales showed significant variations in practice with no consensus on analytical methods, or in the selection of a decision limit for the diagnosis of myocardial damage. Peer review data on assay imprecision at concentrations approaching the analytical limit of detec...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1258/0004563054890006
更新日期:2005-09-01 00:00:00
abstract:BACKGROUND:Neuroendocrine tumours are slow growing tumours known to secrete a variety of vasoactive peptides which give rise to symptoms of the carcinoid syndrome. The diagnosis and monitoring of patients with neuroendocrine tumours is undertaken in many centres using 24 h urinary measurement of 5-hydroxyindoleacetic a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215613109
更新日期:2016-09-01 00:00:00
abstract::The System 1 produced results for glucose and urea which were precise and accurate. It proved suitable as a general laboratory instrument as it samples 28 mul of specimen, is able to calibrate itself, produces results in three minutes, and has the capacity to analyse 56 samples an hour. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327701400179
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples. METHODS:Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium ED...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780480927
更新日期:2007-05-01 00:00:00
abstract::The extent of lipoprotein glycation was assessed in 82 patients diagnosed as diabetes mellitus type II. Glycated lipoprotein levels in serum were determined by agarose gel film electrophoresis in 48 non-diabetic control subjects, 56 diabetics with complications and 26 diabetics with no complications. All glycated lipo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329503200505
更新日期:1995-09-01 00:00:00
abstract::Plasma immunoreactive alpha-atrial natriuretic peptide (IR alpha-ANP) levels were determined before, and at 5 and 10 min after bolus intravenous administration of morphine (0.15 or 0.30 mg/kg) in 21 otherwise healthy human subjects who underwent elective surgery. Five min after injection IR alpha-ANP levels had nearly...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700105
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Various conjugated cholesterol metabolites are excreted in urine of the patients with metabolic abnormalities and hepatobiliary diseases. We aimed to examine the usefulness of precursor ion scan and neutral loss scan for the characterization of conjugated cholesterol metabolites in urine. METHODS:A mixture ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214568871
更新日期:2015-09-01 00:00:00
abstract::The analytical and biological components of variation of total cholesterol, high density lipoprotein (HDL) cholesterol, HDL3 cholesterol, HDL2 cholesterol and apolipoproteins A-I and B in serum were assessed in 12 apparently healthy individuals. All analytes showed marked individuality and therefore conventional popul...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600314
更新日期:1989-05-01 00:00:00
abstract::The last 10 years have witnessed radical changes in the definition and diagnosis of the acute coronary syndrome (ACS), including myocardial infarction, as a result of the introduction of sensitive and specific markers of myocardial damage, the cardiac troponins. One barrier to the universal acceptance of these markers...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456304322664645
更新日期:2004-01-01 00:00:00
abstract::Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456329803500402
更新日期:1998-07-01 00:00:00
abstract::A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900606
更新日期:1992-11-01 00:00:00
abstract::We evaluated a new immunoselective enzyme assay of bone-specific alkaline phosphatase (ALP). The monoclonal antibody used in this assay was raised against purified bone-specific ALP obtained from SAOS-2 human osteosarcoma cell line. Calibration was based on the enzyme's own activity. The relative activity of the antib...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329603300205
更新日期:1996-03-01 00:00:00
abstract:BACKGROUND:Pregnancy represents a major risk factor for deep vein thrombosis (DVT). Most coagulation/fibrinolysis markers currently utilized change during pregnancy, and therefore they cannot accurately evaluate thrombotic events in pregnancy because the rate of false positive results is high. Fibrin monomer complex (F...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307781646076
更新日期:2007-09-01 00:00:00
abstract::Sequential measurements of plasma alkaline phosphatase activity were made in 84 pre-term infants delivered before 38 weeks gestation. In 67% of infants, without evidence of rickets but in whom three or more measurements were made and the peak activity was less than 10 times the adult reference range, the activity rose...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300604
更新日期:1986-11-01 00:00:00
abstract:BACKGROUND:There are few data on oxidative stresses during and after pregnancy, although aggravation of autoimmune disease is implicated in oxidative stress and occurs frequently in the postpartum period. Thioredoxin (TRX) is a stress-inducible protein, and is used as a good biomarker for oxidative stress. To clarify t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2009.009120
更新日期:2010-01-01 00:00:00
abstract::A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or su...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800416
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:The rapid exclusion of acute myocardial infarction in patients with chest pain can reduce the length of hospital admission, prevent unnecessary diagnostic work-up and reduce the burden on our health-care systems. The combined use of biomarkers that are associated with different pathophysiological aspects of ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215578189
更新日期:2015-09-01 00:00:00
abstract::Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327601300125
更新日期:1976-05-01 00:00:00
abstract:BACKGROUND:Measurement of plasma-free metanephrine plus normetanephrine (PFM) is the best screening test for phaeochromocytoma. While clearly raised levels are diagnostic, borderline increases may be associated with factors such as stress and medications, and should prompt a repeat study after interfering factors are w...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.010131
更新日期:2010-11-01 00:00:00
abstract::Background For the early identification of patients at risk of developing diabetic nephropathy, we have developed an ultrasensitive immune complex transfer enzyme immunoassay to measure adiponectin in urine. Methods We developed immune complex transfer enzyme immunoassay for adiponectin and measured urinary adiponecti...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217748681
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:It is recommended that children receiving intravenous fluids should have frequent biochemical monitoring, in some situations 4-6 hourly. Small changes in sodium must be detected, requiring very high precision from sodium analyses. Some children are monitored using venous blood analysed by indirect ion-select...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306778904560
更新日期:2006-11-01 00:00:00