Radioimmunoassay of sodium cromoglycate in human plasma.

Abstract:

:A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the administration of single therapeutic doses of the compound is possible. An antiserum raised in a sheep, radioligand heterogeneously labelled with iodine-125, and a second antibody technique for the separation of bound and free radioligand are employed. The inter-assay coefficient of variation is less than 14% (n = 23). The range of the method is limited; both 0.01 and 0.1 ml volumes of plasma must be analysed to encompass the concentration range 0.93-139 nmol/l which may be encountered in plasma samples from patients and human volunteers. The method is specific for sodium cromoglycate as indicated by a low cross-reactivity of the anti-cromoglycate antiserum with a number of drugs.

journal_name

Ann Clin Biochem

authors

Brown K,Gardner JJ,Lockley WJ,Preston JR,Wilkinson DJ

doi

10.1177/000456328302000105

subject

Has Abstract

pub_date

1983-01-01 00:00:00

pages

31-6

eissn

0004-5632

issn

1758-1001

journal_volume

20 Pt 1

pub_type

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