Abstract:
:Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional methods prevented further explorations. Therefore, we developed a droplet digital polymerase chain reaction (PCR) assay for detecting Fusobacterium nucleatum in stool and investigated its clinical utility in the management of colorectal tumours in a Japanese population. Methods Feces were collected from 60 healthy subjects (control group) and from 11 patients with colorectal non-advanced adenomas (non-advanced adenoma group), 19 patients with colorectal advanced adenoma/carcinoma in situ (advanced adenoma/carcinoma in situ (CIS) group) and 158 patients with colorectal cancer of stages I to IV (colorectal cancer group). Absolute copy numbers of Fusobacterium nucleatum were measured by droplet digital PCR. Results The median copy number of Fusobacterium nucleatum was 17.5 in the control group, 311 in the non-advanced adenoma group, 122 in the advanced adenoma/CIS group, and 317 in the colorectal cancer group. In comparison with that in the control group, the Fusobacterium nucleatum level was significantly higher in the non-advanced adenoma group, the advanced adenoma/CIS group and the colorectal cancer group. Conclusions This study illustrates the potential of stool DNA testing of Fusobacterium nucleatum by droplet digital PCR to detect individuals with colorectal tumours in a Japanese population.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Suehiro Y,Sakai K,Nishioka M,Hashimoto S,Takami T,Higaki S,Shindo Y,Hazama S,Oka M,Nagano H,Sakaida I,Yamasaki Tdoi
10.1177/0004563216643970subject
Has Abstractpub_date
2017-01-01 00:00:00pages
86-91issue
1eissn
0004-5632issn
1758-1001pii
0004563216643970journal_volume
54pub_type
杂志文章abstract:BACKGROUND:It is important to explain target proteins for the understanding of the pathogenesis of vitreoretinal diseases. In a previous study, we identified more than 100 proteins including seven angiogenic-modulated factors in vitreous humours (VHs). Although there have been many reports of expressed protein profiles...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2007.007151
更新日期:2008-05-01 00:00:00
abstract::Insulin resistance is a common condition, recognized to be a central feature of the metabolic syndrome, and strongly associated with an increased risk of cardiovascular disease and diabetes. The quantitative assessment of insulin sensitivity is not used for routine clinical purposes, but the emerging importance of ins...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456307780945778
更新日期:2007-07-01 00:00:00
abstract::Background Measurement of serum dexamethasone during the overnight dexamethasone-suppression test has been recommended to reduce false-positive results when investigating Cushing's syndrome or increasingly commonly found adrenal incidentalomas. Despite this, there remains a paucity of well-validated dexamethasone meth...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218766566
更新日期:2018-11-01 00:00:00
abstract::It is crucially important to detect subarachnoid haemorrhage (SAH) in all patients in whom it has occurred to select patients for angiography and preventative surgery. A computerized tomography (CT) scan is positive in up to 98% of patients with SAH presenting within 12 h, but is positive in only 50% of those presenti...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.007257
更新日期:2008-05-01 00:00:00
abstract:BACKGROUND:In humans, leptin circulates in a free form and is also bound to macromolecules. The aim of the present study was to compare a rapid acid-ethanol precipitation (AEP) method of measuring bound leptin with the more laborious gel exclusion chromatography (GEC) reference procedure. Serum samples collected from h...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303763046157
更新日期:2003-03-01 00:00:00
abstract::Addison's disease is a relatively common disorder to endocrinologists, but is rare and potentially fatal when presenting acutely. Treatment now involves replacement of glucocorticoids and mineralocorticoids with synthetic compounds, although historically patients took common salt and plant-based preparations. We descr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780945624
更新日期:2007-07-01 00:00:00
abstract::The Myolex (Orion) and the RapiTex (Behringwerke) latex agglutination tests for the rapid detection of elevated levels of serum myoglobin were studied prospectively in patients suspected of acute myocardial infarction, who were admitted to hospital within 8 h of pain onset. Using admission blood samples drawn 3.4 +/- ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600309
更新日期:1989-05-01 00:00:00
abstract::Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600606
更新日期:1999-11-01 00:00:00
abstract::We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300509
更新日期:1986-09-01 00:00:00
abstract::The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327601300105
更新日期:1976-01-01 00:00:00
abstract:BACKGROUND:With the recent outbreak in West Africa, hospitals worldwide have been developing protocols for suspect of cases of Ebola virus disease. Patients with Ebola virus disease present with a severe gastroenteritis leading to dehydration and electrolyte abnormalities and as such, routine chemistry analysis is esse...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215581651
更新日期:2016-03-01 00:00:00
abstract::Serum osteocalcin did not show any response to the onset of osteosarcoma in Paget's disease of bone whereas serum alkaline phosphatase increased rapidly. This suggests that osteocalcin is not useful in the diagnosis and management of Paget's osteosarcoma and does not reflect the same osteoblastic processes in bone as ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800216
更新日期:1991-03-01 00:00:00
abstract::Background Measured (MO) and calculated osmotic concentrations (CO) and the osmotic gap (OG) are commonly used in the investigation of electrolyte and volume disturbances as well as in cases of suspected volatile ingestion. Methods We compared 38 published formulae for CO with MO on a large data-set ( n = 9466) and ad...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217694052
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:To date, the difference in microRNA expression profiles in tears of dry eye patients and healthy people has not been reported. In current study, we evaluated the significance of microRNAs and transforming growth factor beta2 (TGF-β2) in distinguishing dry eye. METHODS:A total of 138 patients with dry eye fr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220961746
更新日期:2020-11-01 00:00:00
abstract:BACKGROUND:Method-dependent variation of caeruloplasmin measurement is significant and necessitates the requirement for assay-specific reference intervals. Local determination becomes necessary in the absence of suitable published or manufacturer-quoted reference intervals. METHODS:Applicability of the Beckman Coulter...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563221989360
更新日期:2021-01-27 00:00:00
abstract::Background The aim of the present study is to evaluate the utility of extraction versus non-extraction-based commercial melatonin ELISA kits for determining the melatonin concentration in amniotic fluid obtained in early and late pregnancy. Methods Pregnancy duration less than 28 weeks was defined as early and from 28...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563216645123
更新日期:2017-01-01 00:00:00
abstract::A simple procedure is described for the preparation of a stable precision quality control material for use in the measurement of level of ionised calcium in serum at or near the reference range. Repeat analyses on a Nova 2 ionised calcium analyser of serum pools stored at different temperatures over a period of three ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328101800211
更新日期:1981-03-01 00:00:00
abstract::Whole blood and washed erythrocytes from nutritionally normal and abnormal elderly patients were stored at room temperature, 4 degrees C and -20 degrees C for up to 14 days. In both groups, and under all storage conditions, the erythrocyte transketolase (ETK) activity and the thiamine pyrophosphate (TPP) effect decrea...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200417
更新日期:1985-07-01 00:00:00
abstract::Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400314
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND:Pregnancy represents a major risk factor for deep vein thrombosis (DVT). Most coagulation/fibrinolysis markers currently utilized change during pregnancy, and therefore they cannot accurately evaluate thrombotic events in pregnancy because the rate of false positive results is high. Fibrin monomer complex (F...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307781646076
更新日期:2007-09-01 00:00:00
abstract::Requirements for information technology in pathology now extend well beyond the provision of purely analytical data. With the aim of achieving seamless integration of laboratory data into the total clinical pathway, "informatics"--the art and science of turning data into useful information--is becoming increasingly im...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456304323019532
更新日期:2004-05-01 00:00:00
abstract::Ethylene glycol in plasma, urine or dialysis fluid is analysed as the phenylboronate derivative by mixing with acetonitrile/acidified 2,2-dimethoxypropane containing phenylboronic acid. After centrifugation, a portion of the supernatant is analysed directly by gas-liquid chromatography using a 3% OV-101 column at 150 ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328702400113
更新日期:1987-01-01 00:00:00
abstract::Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327601300125
更新日期:1976-05-01 00:00:00
abstract::Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200135
更新日期:1975-07-01 00:00:00
abstract::A group of international experts prepared two lists of drugs with their serum/plasma and urine concentrations, which should be used when evaluating the performance of a new laboratory method. The two lists were verified by running in vitro interference studies in three European laboratories on Hitachi instruments. The...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1258/0004563011900696
更新日期:2001-07-01 00:00:00
abstract:BACKGROUND:Angiogenesis is involved in the pathogenesis of diabetic retinopathy. Osteoprotegerin, a recently identified glycoprotein belonging to the tumour necrosis factor receptor superfamily, has been implicated to be correlated with angiogenesis. This study aims to determine whether serum and vitreous concentration...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214533669
更新日期:2015-03-01 00:00:00
abstract::The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100206
更新日期:1984-03-01 00:00:00
abstract::An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328201900305
更新日期:1982-05-01 00:00:00
abstract::In a 1 year prospective study we evaluated the effect of feedback of laboratory data on the requesting behaviour of physicians in general medicine. Data on within-hours and out-of-hours clinical chemistry laboratory usage and revenue expenditure for inpatients and outpatients, expressed in terms of clinical workload, ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800204
更新日期:1991-03-01 00:00:00
abstract:BACKGROUND:Placental growth factor (PlGF) and soluble-fms-like tyrosine kinase 1 (sFlt-1) are biomarkers of placental function used to aid the diagnosis and prediction of pregnancy complications. This work verified the analytical performance of both biomarkers and provides preliminary diagnostic accuracy data to identi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220911993
更新日期:2020-05-01 00:00:00