Abstract：
:It is crucially important to detect subarachnoid haemorrhage (SAH) in all patients in whom it has occurred to select patients for angiography and preventative surgery. A computerized tomography (CT) scan is positive in up to 98% of patients with SAH presenting within 12 h, but is positive in only 50% of those presenting within one week. Cerebrospinal fluid (CSF) bilirubin spectrophotometry can be used to determine the need for angiography in those few CT-negative patients in whom clinical suspicion of SAH remains high; it may remain positive up to two weeks after the event. A lumbar puncture (LP) should only be performed >12 h after the onset of presenting symptoms. Whenever possible collect sequential specimens. Always ensure that the least blood-stained CSF sample taken (usually the last) is sent for bilirubin analysis. Protect the CSF from light and avoid vacuum tube transport systems, if possible. Always use spectrophotometry in preference to visual inspection. All CSF specimens are precious and should always be analysed unless insufficient sample is received. Centrifuge the specimen at >2000 rpm for 5 min as soon as possible after receipt in the laboratory. Store the supernatant at 4 degrees C in the dark until analysis. An increase in CSF bilirubin is the key finding, which supports the occurrence of SAH but is not specific for this. In most positive cases, bilirubin will occur with oxyhaemoglobin.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Cruickshank A,Auld P,Beetham R,Burrows G,Egner W,Holbrook I,Keir G,Lewis E,Patel D,Watson I,White P,UK NEQAS Specialist Advisory Group for External Quality Assurance of CSF Proteins and Biochemistry.doi
10.1258/acb.2008.007257subject
Has Abstractpub_date
2008-05-01 00:00:00pages
238-44issue
Pt 3eissn
0004-5632issn
1758-1001pii
45/3/238journal_volume
45pub_type
杂志文章abstract：:The application of a DC plasma emission spectrometer to the measurement of copper, zinc and magnesium in serum and urine is reported. Each assay requires only a simple dilution in 1% nitric acid. Comparisons with standard atomic absorption techniques showed good analytical agreement, especially for copper and magnesiu...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328502200512
更新日期：1985-09-01 00:00:00
abstract：:Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329703400314
更新日期：1997-05-01 00:00:00
abstract：:Eleven unfit bank executives in sedentary employment exercised daily for 10 weeks according to the Canadian Air-Force 5BX Exercise Programme. Over this period no changes were observed in the glucose or insulin responses to a standard breakfast or to intravenous glucose, or in the glucose response to intravenous insuli...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456327901600115
更新日期：1979-03-01 00:00:00
abstract：:Iron status is influenced by inflammation when the normal control of iron metabolism is reorganized by the primary mediators of the acute phase response, tumour necrosis factor-alpha and interleukin-1. The objective of this review is to show how indices of iron status, particularly haemoglobin, serum ferritin and solu...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1258/acb.2007.007167
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haem...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/0004563053492739
更新日期：2005-03-01 00:00:00
abstract：:The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329803500212
更新日期：1998-03-01 00:00:00
abstract：BACKGROUND:Lysophosphatidic acid (LPA) plays important roles in a variety of biological responses, especially in the area of vascular biology, and the determination of its plasma concentration is believed to be important. Several mechanisms are known to be involved in the metabolism of LPA. METHODS:To identify factors...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2008.007242
更新日期：2008-07-01 00:00:00
abstract：:Serum concentrations of total thyroid hormones, thyroid hormone binding proteins, free thyroid hormones and thyroid stimulating hormone (TSH) were measured in a large group of healthy normal subjects (n = 218) to determine the influence of age and sex on tests of thyroid function. No effect of age on measurements of t...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328502200506
更新日期：1985-09-01 00:00:00
abstract：BACKGROUND:Oxidative stress may play an aetiological role in the development and progression of cardiovascular disease (CVD). However, evidence on its biochemical markers has been controversial. This article aimed to assess the role of F2-isoprostanes, a marker for measuring in vivo lipid oxidation, as a biomarker for ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1258/acb.2012.011263
更新日期：2013-03-01 00:00:00
abstract：BACKGROUND:Measurement of HbA(1c) is the standard test for assessment of glycaemic control in diabetic subjects. Using new glucose sensing technology we re-evaluated the significance of HbA(1c) in terms of the aspects of the blood profile it measures in patients with diabetes. METHODS:In a group of 27 patients with ty...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/000456302320314557
更新日期：2002-09-01 00:00:00
abstract：:Most thyroid-stimulating hormone (TSH) assays now have the sensitivity required by thyroid guidelines and allow the reliable identification of patients with both overt and subclinical hyperthyroidism. Clinical guidelines usually quote decision limits for TSH, but often ignore the issue of whether variability in bias b...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/000456307780480945
更新日期：2007-05-01 00:00:00
abstract：:The analysis of serum albumin by bromocresol purple (BCP), by bromocresol green (BCG), and by electroimmunoassay (EIA) has been compared in a mixed group of 100 hospital patients. Good agreement between the immunochemical and BCP methods, but a positive bias with the BCG method led to routine use of the BCP method. In...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328502200314
更新日期：1985-05-01 00:00:00
abstract：BACKGROUND:Circulating levels of bioavailable oestradiol and testosterone are often desirable for clinical practice or investigational studies of children. However, assays to measure circulating hormone levels might not always be accessible. We sought to validate the empirical calculation of circulating bioavailable te...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1258/acb.2010.009112
更新日期：2010-05-01 00:00:00
abstract：:A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456327501200102
更新日期：1975-01-01 00:00:00
abstract：:It is our aim to summarise and discuss procedures for the evaluation of the concentration of free ionised calcium in serum or plasma. Stress is laid upon the interrelations and relative validity of the most common algebraic expressions to appraise the calcium status. The multitude of formulae proposed in the literatur...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1177/000456328402100501
更新日期：1984-09-01 00:00:00
abstract：:The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328402100206
更新日期：1984-03-01 00:00:00
abstract：BACKGROUND:Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipept...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1177/0004563218796860
更新日期：2019-01-01 00:00:00
abstract：:We have evaluated the clinical relevance of monitoring acute phase proteins in severe urinary tract infection. Body temperature, white blood cell count, erythrocyte sedimentation rate, serum amyloid A protein (SAA), C-reactive protein (CRP), alpha-1-antichymotrypsin (ACT) and alpha-1-acid glycoprotein (AGP) were deter...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329303000308
更新日期：1993-05-01 00:00:00
abstract：:The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329803500507
更新日期：1998-09-01 00:00:00
abstract：BACKGROUND:With the recent outbreak in West Africa, hospitals worldwide have been developing protocols for suspect of cases of Ebola virus disease. Patients with Ebola virus disease present with a severe gastroenteritis leading to dehydration and electrolyte abnormalities and as such, routine chemistry analysis is esse...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563215581651
更新日期：2016-03-01 00:00:00
abstract：:The activity of serum deoxycytidylate deaminase, not hitherto reported to increase after myocardial infarction, has been investigated and the results have been related to other cardiac enzymes, including the MB fraction of creatine phosphokinase. Deoxycytidylate deaminase was found to increase to maximum activity on t...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456327801500175
更新日期：1978-11-01 00:00:00
abstract：:A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protecte...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328602300410
更新日期：1986-07-01 00:00:00
abstract：:The two stages of a two-site immunoradiometric assay were investigated separately. In the first stage, the amount of ferritin bound to coated tubes initially showed a rapid increase with increasing concentration of added ferritin. This was followed by a plateau and then a further increase which appeared to be largely ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328402100510
更新日期：1984-09-01 00:00:00
abstract：:Hypogonadism in the male results from inadequate testicular function, especially defects in androgen synthesis and secretion, or action. Androgen action is important throughout normal male development: in the fetus, puberty, adult life and old age. Regulation is by variable activity of the hypothalamo-pituitary axis a...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1258/acb.2010.010150
更新日期：2010-11-01 00:00:00
abstract：:Haptoglobin is an acute-phase protein with three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2. Studies have shown that functional differences between these phenotypes have important consequences in a number of pathological disorders (e.g. cardiovascular disease, autoimmune disorders, infectious disease), making phenoty...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329903600507
更新日期：1999-09-01 00:00:00
abstract：:A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or su...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329102800416
更新日期：1991-07-01 00:00:00
abstract：:The osmolality of plasma and heparinised whole blood samples collected from hospital patients was estimated using measurement of the depression of freezing point. There was no clinically significant difference between osmolality measurement made on either whole blood, or plasma taken from the same patient. Neither cel...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328602300115
更新日期：1986-01-01 00:00:00
abstract：:Parenteral nutrition is a well-established technique for feeding patients with intestinal failure and other conditions which render enteral feeding impossible. Biochemical tests are required in the initial assessment of these patients, for monitoring their response to treatment and for the detection and management of ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1177/000456328702400401
更新日期：1987-07-01 00:00:00
abstract：:The aim of this study was to assess the performance of a commercially available procedure for detecting anti-Sjögren's syndrome A (anti-SSA) and anti-Sjögren's syndrome B (anti-SSB) antibodies by immunoblotting (IB) and compare it with double immunodiffusion (DID). We also studied the clinical significance of these pr...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329903600310
更新日期：1999-05-01 00:00:00
abstract：:Thyroglobulin (Tg) is a tumour marker for differentiated thyroid cancer. Interpretation requires a knowledge of the current thyrotropin (TSH) concentration as secretion is TSH-dependent. While a raised serum Tg may be indicative of residual or recurrent thyroid cancer, trauma to the thyroid (e.g. surgical, biopsy or d...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1258/acb.2011.011248
更新日期：2012-07-01 00:00:00
