Mind the gap: shortcomings of the osmotic gap and a possible solution.

abstract：BACKGROUND:The rapid exclusion of acute myocardial infarction in patients with chest pain can reduce the length of hospital admission, prevent unnecessary diagnostic work-up and reduce the burden on our health-care systems. The combined use of biomarkers that are associated with different pathophysiological aspects of ...

journal_title：Annals of clinical biochemistry

authors： Jacobs LH,van Borren M,Gemen E,van Eck M,van Son B,Glatz JF,Daniels M,Kusters R

更新日期：2015-09-01 00:00:00

abstract：:Background The aim of the present study is to evaluate the utility of extraction versus non-extraction-based commercial melatonin ELISA kits for determining the melatonin concentration in amniotic fluid obtained in early and late pregnancy. Methods Pregnancy duration less than 28 weeks was defined as early and from 28...

journal_title：Annals of clinical biochemistry

authors： Bagci S,Altuntas Ö,Katzer D,Berg C,Willruth A,Reutter H,Bartmann P,Müller A,Zur B

更新日期：2017-01-01 00:00:00

abstract：:Eleven unfit bank executives in sedentary employment exercised daily for 10 weeks according to the Canadian Air-Force 5BX Exercise Programme. Over this period no changes were observed in the glucose or insulin responses to a standard breakfast or to intravenous glucose, or in the glucose response to intravenous insuli...

journal_title：Annals of clinical biochemistry

authors： Moore RA,Penfold WA,Simpson RD,Simpson RW,Mann JI,Turner RC

更新日期：1979-03-01 00:00:00

abstract：:The System 1 produced results for glucose and urea which were precise and accurate. It proved suitable as a general laboratory instrument as it samples 28 mul of specimen, is able to calibrate itself, produces results in three minutes, and has the capacity to analyse 56 samples an hour. ...

journal_title：Annals of clinical biochemistry

authors： Potezny N,Quelch J,Fuller B,Geary TD

更新日期：1977-09-01 00:00:00

abstract：:This paper summarises the views of the authors on the provision of a prolactin assay service. We discuss the pathophysiology of prolactin secretion and the clinical indications that arise from that. We cover the rather complex issue of the definition of normal and elevated prolactin levels. From these considerations, ...

journal_title：Annals of clinical biochemistry

authors： Jeffcoate SL,Bacon RR,Beastall GH,Diver MJ,Franks S,Seth J

更新日期：1986-11-01 00:00:00

abstract：:The effect of oral vitamin E supplementation on the oxido-reductive status of red blood cells in normal mice and those subject to oxidative stress by chronic administration of the anti-tumour drug Adriamycin was investigated. Mice were randomly separated into three groups of 20 animals each and maintained on diets ide...

journal_title：Annals of clinical biochemistry

authors： Thabrew MI,Samarawickrema N,Chandrasena LG,Jayasekera S

更新日期：1999-03-01 00:00:00

abstract：:A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...

journal_title：Annals of clinical biochemistry

authors： Willemsen JJ,Ross HA,Wolthers BG,Sweep CG,Kema IP

更新日期：2001-11-01 00:00:00

abstract：:A recently introduced turbidimetric immunoassay using shell/core particles for determination of myoglobin (Turbiquant Myoglobin, Behringwerke, Marburg, Germany) was evaluated on the Behring Turbitimer analyzer. Intra-assay CV varied between 1.0% and 3.2%; interassay CV was between 2.0% and 3.6%. Linear results were ob...

journal_title：Annals of clinical biochemistry

authors： Delanghe J,Chapelle JP,el Allaf M,De Buyzere M

更新日期：1991-09-01 00:00:00

abstract：:Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated p...

journal_title：Annals of clinical biochemistry

authors： Menéndez LG,Fernández AL,Enguix A,Ciriza C,Amador J

更新日期：2001-05-01 00:00:00

abstract：BACKGROUND:Glycated haemoglobin (HbA1c) concentration is lower relative to glyacemic control in diabetic patients on haemodialysis. However, it is unknown as to whether this is also true for diabetic patients with end-stage renal disease but not on haemodialysis. METHODS:Correlations between HbA1c or glycated albumin ...

journal_title：Annals of clinical biochemistry

authors： Shima K,Chujo K,Yamada M,Komatsu M,Noma Y,Mizuguchi T

更新日期：2012-01-01 00:00:00

abstract：:A rapid and simple method for detecting circulating immune complexes based on turbidity measurements following polyethylene glycol precipitation was studied with regard to its suitability as a routine assay in clinical laboratories. This method was found to have an acceptable degree of precision provided the temperatu...

journal_title：Annals of clinical biochemistry

authors： Kilpatrick DC,Weston J,Irvine WJ

更新日期：1981-11-01 00:00:00

abstract：BACKGROUND:To date, the difference in microRNA expression profiles in tears of dry eye patients and healthy people has not been reported. In current study, we evaluated the significance of microRNAs and transforming growth factor beta2 (TGF-β2) in distinguishing dry eye. METHODS:A total of 138 patients with dry eye fr...

journal_title：Annals of clinical biochemistry

authors： Wang Q,Xie X,Li H,Hao S

更新日期：2020-11-01 00:00:00

abstract：:Background Point-of-care testing has been developed to provide rapid test results. Most published studies focus on analytical performance, neglecting its impact on patient outcomes. Objective To review the analytical performance and accuracy of point-of-care testing specifically planned for immunoassay and to evaluate...

journal_title：Annals of clinical biochemistry

authors： Pecoraro V,Banfi G,Germagnoli L,Trenti T

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:In humans, leptin circulates in a free form and is also bound to macromolecules. The aim of the present study was to compare a rapid acid-ethanol precipitation (AEP) method of measuring bound leptin with the more laborious gel exclusion chromatography (GEC) reference procedure. Serum samples collected from h...

journal_title：Annals of clinical biochemistry

authors： Jamieson NB,McMillan DC,Brown DJ,Wallace AM

更新日期：2003-03-01 00:00:00

abstract：BACKGROUND:Calculated globulin fraction is derived from the liver function tests by subtracting albumin from the total protein. Since immunoglobulins comprise the largest component of the serum globulin concentration, increased or decreased calculated globulins and may identify patients with hypogammaglobulinaemia or h...

journal_title：Annals of clinical biochemistry

authors： Hoo T,Lim E,John M,D'Orsogna L,McLean-Tooke A

更新日期：2021-01-11 00:00:00

abstract：:Homocysteine, a sulphur amino acid, is a branch-point intermediate of methionine metabolism. It can be degraded in the transsulphuration pathway to cystathionine, or remethylated to methionine via the remethylation pathway. In both pathways, major genetic defects that cause enzyme deficiencies are associated with very...

journal_title：Annals of clinical biochemistry

authors： Lievers KJ,Kluijtmans LA,Blom HJ

更新日期：2003-01-01 00:00:00

abstract：:Mass spectrometry has for many years enabled us to rapidly identify and quantify many different compounds in biological samples. The equipment is currently available in specialist centres investigating metabolic disorders and in toxicology laboratories. Improvements in sample introduction and refinements in the mass s...

journal_title：Annals of clinical biochemistry

authors： Honour JW

更新日期：2003-11-01 00:00:00

abstract：:A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protecte...

journal_title：Annals of clinical biochemistry

authors： Pickard CE,Stewart AD,Hartley R,Lucock MD

更新日期：1986-07-01 00:00:00

abstract：:The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...

journal_title：Annals of clinical biochemistry

authors： Crook MA,Velauthar U,Moran L,Griffiths W

更新日期：1999-03-01 00:00:00

abstract：BACKGROUND:The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the ...

journal_title：Annals of clinical biochemistry

authors： McEneny J,McPherson PA,McGinty A,Hull SS,McCance DR,Young IS

更新日期：2013-01-01 00:00:00

abstract：:The immunosuppressive agent mycophenolate mofetil is a prodrug for the active compound mycophenolic acid. Following organ transplantation, it is used mostly in combination with other immunosuppressive drugs, but it has also been used as primary therapy. Although in the pivotal clinical studies the drug was given in fi...

journal_title：Annals of clinical biochemistry

authors： Holt DW

更新日期：2002-05-01 00:00:00

abstract：:Background Accumulating evidence shows an over-abundance of Fusobacterium nucleatum in colorectal tumour tissues. Although stool DNA testing of Fusobacterium nucleatum might be a potential marker for the detection of colorectal tumours, the difficulty in detecting Fusobacterium nucleatum in stool by conventional metho...

journal_title：Annals of clinical biochemistry

authors： Suehiro Y,Sakai K,Nishioka M,Hashimoto S,Takami T,Higaki S,Shindo Y,Hazama S,Oka M,Nagano H,Sakaida I,Yamasaki T

更新日期：2017-01-01 00:00:00

abstract：:Two cases of hypercholesterolaemic patients are presented in whom raised plasma creatine kinase was noted during treatment with statins. The plasma creatine kinase failed to fall following cessation of therapy. Further investigation revealed the aetiology of the raised plasma creatine kinase to be due to previously un...

journal_title：Annals of clinical biochemistry

authors： Barth JH,Brownjohn AM,Jamieson DR

更新日期：2003-09-01 00:00:00

abstract：:A 41-year-old lady presented with classical symptoms of a phaeochromocytoma and markedly elevated urinary adrenaline concentration. Urinary fractionated metanephrine and normetanephrine concentrations were within the normal reference range. Computed tomography of the abdomen/pelvis and I(123) meta-iodobenzylguanidine ...

journal_title：Annals of clinical biochemistry

authors： King TF,Shea PO,Sullivan EP,Srinivasan R,Griffin A,Fitzgerald R,Tormey W,Smith D

更新日期：2008-03-01 00:00:00

abstract：BACKGROUND:Macroprolactin is a high molecular mass complex of prolactin that may be the cause of elevated serum prolactin as determined by immunoassay. The composition of macroprolactin and its reactivity in immunoassays are variable but the complex has minimal bioactivity in vivo. Hyperprolactinaemia due to unrecogniz...

journal_title：Annals of clinical biochemistry

authors： Ellis AR,Fahie-Wilson M,Axcell M,Sands K,Hill RP

更新日期：2006-01-01 00:00:00

abstract：:Parenteral nutrition is a well-established technique for feeding patients with intestinal failure and other conditions which render enteral feeding impossible. Biochemical tests are required in the initial assessment of these patients, for monitoring their response to treatment and for the detection and management of ...

journal_title：Annals of clinical biochemistry

authors： Marshall WJ,Mitchell PE

更新日期：1987-07-01 00:00:00

abstract：BACKGROUND:Emergency abdominal surgery carries considerable postoperative morbidity and mortality. Hypovolaemia is considered to be a cause of renal hypoperfusion, which is associated with a decreased clearance of serum urea and creatinine. This study examines whether the perioperative serum urea and creatinine concent...

journal_title：Annals of clinical biochemistry

authors： Harten J,Hay A,McMillan DC,McArdle CS,O'Reilly DS,Kinsella J

更新日期：2006-07-01 00:00:00

abstract：BACKGROUND:Patient-based real-time quality control (PBRTQC) techniques have been described in clinical chemistry for over 50 years. PBRTQC has a number of advantages over traditional quality control including commutability, cost and the opportunity for real-time monitoring. However, there are few systematic investigati...

journal_title：Annals of clinical biochemistry

authors： Smith JD,Badrick T,Bowling F

更新日期：2020-05-01 00:00:00

abstract：BACKGROUND:In the enzyme reaction for the determination of the unbound (free) bilirubin concentration by glucose oxidase and peroxidase, materials with low affinity for serum protein are reactive. The influence of these materials on the determination of serum unbound bilirubin was investigated. METHODS Serum samples fr...

journal_title：Annals of clinical biochemistry

authors： Itoh S,Kawada K,Kusaka T,Yasuda S,Okada H,Imai T,Isobe K

更新日期：2002-11-01 00:00:00

abstract：BACKGROUND:There are few data on oxidative stresses during and after pregnancy, although aggravation of autoimmune disease is implicated in oxidative stress and occurs frequently in the postpartum period. Thioredoxin (TRX) is a stress-inducible protein, and is used as a good biomarker for oxidative stress. To clarify t...

journal_title：Annals of clinical biochemistry

authors： Kuroda S,Watanabe M,Santo T,Shimizuishi Y,Takano T,Hidaka Y,Kimura T,Iwatani Y

更新日期：2010-01-01 00:00:00