Abstract：
BACKGROUND:To date, the difference in microRNA expression profiles in tears of dry eye patients and healthy people has not been reported. In current study, we evaluated the significance of microRNAs and transforming growth factor beta2 (TGF-β2) in distinguishing dry eye. METHODS:A total of 138 patients with dry eye from October 2017 to October 2018 were selected. During the same period, 138 healthy persons were collected. All patients were followed up for 12 months through outpatient, telephone or medical records and the time of corneal injury was recorded. RESULTS:Compared with healthy people, TGF-β2 concentrations in dry eye patients were significantly decreased (P < 0.05). Array analysis, predictive software and dual-luciferase reporter assays showed that miR-450b-5p, miR-1283 and miR-3671 can target TGF-β2 expression. Tear miR-450b-5p, miR-1283 and miR-3671 concentrations were significantly higher in dry eye patients than healthy people. A logistic regression model combining miR-450b-5p, miR-1283, miR-3671 and TGF-β2 was performed. This model presented a high discriminating value (AUC: 0.907, 0.876-0.939, P < 0.001) than any single indicator, and the sensitivity and specificity were 77.7% and 92.7%, respectively. Compared with the low miR-450b-5p, low miR-1283, low miR-3671 and high TGF-β2 groups, the high miR-450b-5p, high miR-1283, high miR-3671 and low TGF-β2 groups had a significantly higher probability of corneal injury (TGF-β2: χ2 = 5.762, P = 0.016; miR-450b-5p: χ2 = 13.267, P < 0.001; miR-1283: χ2 = 19.431, P < 0.001; miR-3671: χ2 = 8.131, P = 0.004). CONCLUSION:Current model combining tear miR-450b-5p, miR-1283, miR-3671 and TGF-β2 had important values in the identification of dry eye and was of great value in evaluating the risk of corneal injury.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Wang Q,Xie X,Li H,Hao Sdoi
10.1177/0004563220961746subject
Has Abstractpub_date
2020-11-01 00:00:00pages
420-428issue
6eissn
0004-5632issn
1758-1001journal_volume
57pub_type
杂志文章abstract：BACKGROUND:Current guidelines define acute myocardial infarction (AMI) by the rise and/or fall of cardiac troponin with ≥1 value above the 99th percentile. Past troponin assays have been unreliable at the lower end of the range. Highly sensitive assays have therefore been developed to increase the clinical sensitivity ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2010.010219
更新日期：2011-05-01 00:00:00
abstract：:The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456327601300105
更新日期：1976-01-01 00:00:00
abstract：:Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1177/0004563216674743
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563220921884
更新日期：2020-05-01 00:00:00
abstract：:Hereditary renal hypouricaemia (HRH) is a genetic disorder commonly associated with exercise-induced acute kidney injury (AKI). We report the case of a 19-year-old man who was admitted to hospital with exercise-induced AKI and who was subsequently shown to have HRH. We believe this to be the first description of a cas...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2012.012017
更新日期：2013-05-01 00:00:00
abstract：:A number of cardiac interventional procedures are available for the treatment of angina, including percutaneous transluminal coronary angioplasty (PTCA), stent insertion and rotational atherectomy (RA). Variable degrees of myocardial cell injury during PTCA and stent insertion have been observed, based on rises in cre...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/0004563001900075
更新日期：2000-11-01 00:00:00
abstract：BACKGROUND AND AIM:Familial hypercholesterolaemia is caused by variants in the low-density lipoprotein cholesterol metabolic pathway involving LDLR, APOB and PCSK9 genes. A national genetic testing service in Wales, UK has observed that no familial hypercholesterolaemia variant is found in almost 80% patients with the ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563218793165
更新日期：2019-01-01 00:00:00
abstract：:Background Analysis of dried blood specimens has been an integral part of laboratory medicine dating back to the early 1960s when they were introduced as part of neonatal screening programs. More recently, they have been used in Down syndrome screening programmes. Dried blood spot specimens can be collected either by ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563213484906
更新日期：2013-11-01 00:00:00
abstract：BACKGROUND:Placental growth factor (PlGF) and soluble-fms-like tyrosine kinase 1 (sFlt-1) are biomarkers of placental function used to aid the diagnosis and prediction of pregnancy complications. This work verified the analytical performance of both biomarkers and provides preliminary diagnostic accuracy data to identi...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563220911993
更新日期：2020-05-01 00:00:00
abstract：:The calculation of within-laboratory imprecision in quality-assessment (QA) programmes normally involves combining data from different analyte concentrations to calculate an average standard deviation (SD) or coefficient of variation. However, for immunoassay neither of these parameters is concentration independent. T...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328402100611
更新日期：1984-11-01 00:00:00
abstract：BACKGROUND:Oxidation of lipoproteins is thought to play a crucial role in atherogenesis. Role for triglyceride-rich lipoproteins in atherogenesis is unclear. Thus, we aimed to investigate whether cholesteryl ester hydroperoxides (CEOOH) are present in very low-density lipoproteins (VLDL) and intermediate-density lipopr...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563213516093
更新日期：2014-11-01 00:00:00
abstract：:Lack of accuracy in expressing concentrations of specific proteins in different lots of Hyland calibrating reference and quality control materials, supplied for the Hyland PDQ laser nephelometer, prompted an attempt to find alternative and more reliable calibrating materials available commercially. Laser grade (Atlant...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328001700403
更新日期：1980-07-01 00:00:00
abstract：:A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329202900606
更新日期：1992-11-01 00:00:00
abstract：:Improvement in the control of diabetic patients is aided by a knowledge of blood glucose levels during a 'normal' (non-hospitalised) day. We have devised a 5 microliter capillary tube collection system as a 'kit' for home use by diabetics. Blood collected into 5 microliter capillary tubes is washed into a protein prec...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328101800507
更新日期：1981-09-01 00:00:00
abstract：:Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in i...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563217700172
更新日期：2018-03-01 00:00:00
abstract：:A bloodspot assay for testosterone has been developed using an antiserum raised against testosterone-3-carboxymethyloxime-bovine serum albumin (T-3 CMO-BSA) conjugate and 125I-T-3CMO-histamine tracer. The method has a detection limit of 0.4 nmol/L with between-batch precision of less than 15% for testosterone values b...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329903600411
更新日期：1999-07-01 00:00:00
abstract：BACKGROUND:Method-dependent variation of caeruloplasmin measurement is significant and necessitates the requirement for assay-specific reference intervals. Local determination becomes necessary in the absence of suitable published or manufacturer-quoted reference intervals. METHODS:Applicability of the Beckman Coulter...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/0004563221989360
更新日期：2021-01-27 00:00:00
abstract：:An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328201900305
更新日期：1982-05-01 00:00:00
abstract：:Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328702400512
更新日期：1987-09-01 00:00:00
abstract：BACKGROUND:Measurement of serum natriuretic peptides is recommended in patients with suspected heart failure. Assays for N-terminal pro-B-type natriuretic peptide (NT-proBNP) are available on several platforms and can be measured in serum or heparinized plasma. Siemens Healthcare Diagnostics do not recommend the use of...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2012.012053
更新日期：2012-09-01 00:00:00
abstract：:Serum osteocalcin did not show any response to the onset of osteosarcoma in Paget's disease of bone whereas serum alkaline phosphatase increased rapidly. This suggests that osteocalcin is not useful in the diagnosis and management of Paget's osteosarcoma and does not reflect the same osteoblastic processes in bone as ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329102800216
更新日期：1991-03-01 00:00:00
abstract：BACKGROUND:Oxidative stress may play an aetiological role in the development and progression of cardiovascular disease (CVD). However, evidence on its biochemical markers has been controversial. This article aimed to assess the role of F2-isoprostanes, a marker for measuring in vivo lipid oxidation, as a biomarker for ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1258/acb.2012.011263
更新日期：2013-03-01 00:00:00
abstract：:We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples cl...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456329703400206
更新日期：1997-03-01 00:00:00
abstract：:The content of cytoplasmic 17 beta oestradiol and progesterone receptors in human uterine leiomyoma and normal myometrium in the Negroid population was determined. Eighteen women of reproductive age, at various stages of the menstrual cycle, were included in the study. The serum oestrogen and progesterone concentratio...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1177/000456328702400304
更新日期：1987-05-01 00:00:00
abstract：:The magnitude of the inflammatory response to surgery depends on the degree of injury during surgical procedures. Laparoscopic techniques are generally associated with less postoperative pain and shorter hospital stay compared with open procedures, presumably due to less tissue injury and reduced inflammatory response...
journal_title：Annals of clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1177/000456329703400509
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:Macroprolactin is a high molecular mass complex of prolactin that may be the cause of elevated serum prolactin as determined by immunoassay. The composition of macroprolactin and its reactivity in immunoassays are variable but the complex has minimal bioactivity in vivo. Hyperprolactinaemia due to unrecogniz...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/000456306775141830
更新日期：2006-01-01 00:00:00
abstract：BACKGROUND:Golgi protein-73 (GP73) is a newly identified candidate serum marker for liver diseases. The utility of this biomarker remains limited, largely due to the lack of quantitative information. The aims of this study were to quantify serum GP73 (sGP73) in healthy individuals and in patients with liver diseases, a...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2008.008088
更新日期：2009-01-01 00:00:00
abstract：:A 41-year-old lady presented with classical symptoms of a phaeochromocytoma and markedly elevated urinary adrenaline concentration. Urinary fractionated metanephrine and normetanephrine concentrations were within the normal reference range. Computed tomography of the abdomen/pelvis and I(123) meta-iodobenzylguanidine ...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章
doi：10.1258/acb.2007.007106
更新日期：2008-03-01 00:00:00
abstract：:A method for the determination of metanephrine (MN; also known as metadrenaline), normetanephrine (NMN; also known as normetadrenaline) and 3-methoxytyramine (3-MT) in human urine using high-performance liquid chromatography followed by electrochemical detection (ECD) was validated primarily by comparing the results w...
journal_title：Annals of clinical biochemistry
pub_type： 更正并重新发布的文章,杂志文章
doi：10.1258/0004563011900984
更新日期：2001-11-01 00:00:00
abstract：:Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...
journal_title：Annals of clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1177/000456329803500402
更新日期：1998-07-01 00:00:00
