Measurement of ethylene glycol (ethane-1,2-diol) in biological specimens using derivatisation and gas-liquid chromatography with flame ionisation detection.

abstract：:We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are...

journal_title：Annals of clinical biochemistry

authors： Barenghi L,Ceriotti F,Luzzana M,Ripamonti M,Mosca A,Bonini PA

更新日期：1986-09-01 00:00:00

abstract：:Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...

journal_title：Annals of clinical biochemistry

authors： Meek CL

更新日期：2017-01-01 00:00:00

abstract：:591 patients with a history of coronary heart disease had one or more biochemical markers of tobacco smoking measured. 26% were self reported smokers and a further 4% were apparent 'smoking deceivers'. The urinary nicotine metabolite concentration is an excellent marker for tobacco smoking; breath CO would be a suitab...

journal_title：Annals of clinical biochemistry

authors： Archbold GP,Cupples ME,McKnight A,Linton T

更新日期：1995-03-01 00:00:00

abstract：BACKGROUND:Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. METHODS:We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, us...

journal_title：Annals of clinical biochemistry

authors： Ishikawa H,Matsuzawa T,Ohashi K,Nagamura Y

更新日期：2003-05-01 00:00:00

abstract：:The measurement of galactose-1-phosphate has been advocated for monitoring galactosaemia. A radioenzymatic method is described in which red-cell haemolysates are incubated with radiolabelled uridine diphospho-glucose and exogenous galactose-1-phosphate uridyl transferase. Labelled glucose-1-phosphate is formed in prop...

journal_title：Annals of clinical biochemistry

authors： Dobbie JA,Holton JB

更新日期：1986-05-01 00:00:00

abstract：:We conducted an electrophoretic analysis of monoclonal gamma-globulin found in the serum of a patient with splenic marginal-zone lymphoma. This monoclonal protein showed electrophoretic mobility to the gamma region and reacted with anti-immunoglobulin (IgG) antiserum but not with anti-kappa or anti-lambda light chain ...

journal_title：Annals of clinical biochemistry

authors： Morita K,Kawamoto H,Takada H,Nakamura S,Ishii K,Okamoto Y

更新日期：2006-03-01 00:00:00

abstract：:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...

journal_title：Annals of clinical biochemistry

authors： Green B,Cooper JD,Turnell DC

更新日期：1989-07-01 00:00:00

abstract：:Heterophilic antibodies are well described, but poorly appreciated interferents and is often not a recognized problem affecting most immunoassays. We report a patient presented with ectopic Cushing's syndrome (CS), but repeated plasma adrenocorticotropic hormone (ACTH) concentrations conducted by immunoassay were inap...

journal_title：Annals of clinical biochemistry

authors： Saiegh L,Odeh M,Chen-Konak L,Elias N,Sheikh-Ahmad M,Reut M,Slobodin G,Bejar J,Shechner C

更新日期：2014-07-01 00:00:00

abstract：:Protoporphyrin and zinc-protoporphyrin were measured in the erythrocytes of normal subjects, workers exposed to lead and patients with iron deficiency and erythropoietic protoporphyria (EPP). Results showed significantly higher levels of zinc-protoporphyrin in the lead-exposed workers (P < 0.0001), patients with iron ...

journal_title：Annals of clinical biochemistry

authors： Nelson JC,Westwood M,Allen KR,Newton KE,Barth JH

更新日期：1998-05-01 00:00:00

abstract：:Fasting glycosylated haemoglobin was analysed in 535 consecutive patients having an oral 75 g glucose tolerance test for diagnostic purposes. A reference range for fasting glycosylated haemoglobin was established from patients with a non-diabetic glucose tolerance test as defined by the World Health Organisation Exper...

journal_title：Annals of clinical biochemistry

authors： Albutt EC,Nattrass M,Northam BE

更新日期：1985-01-01 00:00:00

abstract：PURPOSE:We assessed erythrocyte sedimentation rate and high-sensitivity C-reactive protein concentration in knee osteoarthritis and non-knee osteoarthritis. In addition, we investigated potential relationship between the levels of erythrocyte sedimentation rate and high-sensitivity C-reactive protein with clinical find...

journal_title：Annals of clinical biochemistry

authors： Hanada M,Takahashi M,Furuhashi H,Koyama H,Matsuyama Y

更新日期：2016-09-01 00:00:00

abstract：:The Myolex (Orion) and the RapiTex (Behringwerke) latex agglutination tests for the rapid detection of elevated levels of serum myoglobin were studied prospectively in patients suspected of acute myocardial infarction, who were admitted to hospital within 8 h of pain onset. Using admission blood samples drawn 3.4 +/- ...

journal_title：Annals of clinical biochemistry

authors： Konings CH,Funke Küpper AJ,Verheugt FW

更新日期：1989-05-01 00:00:00

abstract：:Quadriceps muscle specimens from autopsy of 28 neonates (gestational age 25-42 weeks) were investigated to determine pyruvate and malate oxidation rates and several enzymes of the mitochondrial oxidative process. In general, the levels of all mitochondrial parameters measured, including carnitine levels, were lower in...

journal_title：Annals of clinical biochemistry

authors： Sperl W,Sengers RC,Trijbels JM,Ruitenbeek W,Doesburg WH,Smeitink JA,Kollée LA,Boon JM

更新日期：1992-11-01 00:00:00

abstract：:This review describes the development and application of a novel test to determine levels of human immunoreactive trypsin, an enzyme produced solely by the pancreas, in biological fluids. Being organ-specific, the assay of immunoreactive trypsin should be an ideal marker of pancreatic function, and this is supported b...

journal_title：Annals of clinical biochemistry

authors： Stagg BH,Wood TP

更新日期：1979-05-01 00:00:00

abstract：:Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-...

journal_title：Annals of clinical biochemistry

authors： Yoshimoto S,Hirota M,Ohboshi C,Shima K

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:Calculated globulin fraction is derived from the liver function tests by subtracting albumin from the total protein. Since immunoglobulins comprise the largest component of the serum globulin concentration, increased or decreased calculated globulins and may identify patients with hypogammaglobulinaemia or h...

journal_title：Annals of clinical biochemistry

authors： Hoo T,Lim E,John M,D'Orsogna L,McLean-Tooke A

更新日期：2021-01-11 00:00:00

abstract：:We examined the reproducibility of oral glucose tolerance tests (OGTT) using the World Health Organization criterion in 212 Chinese subjects (male 149, female 63) who underwent two 75 g OGTTs within a 6-week period. The overall reproducibility was 65.6% (139/212) of which 74 subjects had normal glucose tolerance, 24 h...

journal_title：Annals of clinical biochemistry

authors： Ko GT,Chan JC,Woo J,Lau E,Yeung VT,Chow CC,Cockram CS

更新日期：1998-01-01 00:00:00

abstract：:Sequential measurements of plasma alkaline phosphatase activity were made in 84 pre-term infants delivered before 38 weeks gestation. In 67% of infants, without evidence of rickets but in whom three or more measurements were made and the peak activity was less than 10 times the adult reference range, the activity rose...

journal_title：Annals of clinical biochemistry

authors： Walters EG,Murphy JF,Henry P,Gray OP,Elder GH

更新日期：1986-11-01 00:00:00

abstract：:Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...

journal_title：Annals of clinical biochemistry

authors： Deverill I,Lock RJ

更新日期：1983-07-01 00:00:00

abstract：:A binding assay for sex hormone binding globulin (SHBG) has been developed in which SHBG is saturated with tritiated dihydrotestosterone (DHT). Separation of bound and free DHT is achieved by using Concanavalin A-Sepharose as a solid phase matrix. The method is described and its performance, including linearity, impre...

journal_title：Annals of clinical biochemistry

authors： Whittaker JA,Cawood ML,Oakey RE

更新日期：1992-03-01 00:00:00

abstract：BACKGROUND:We evaluated procalcitonin (PCT) assay in the emergency diagnosis of neonatal bacterial infection, especially in preterm infants, relative to C-reactive protein (CRP) and fibrinogen. METHODS:One hundred and twenty neonates (32 preterm), of whom 21 were infected, were tested. RESULTS:Concentrations of PCT, ...

journal_title：Annals of clinical biochemistry

authors： Guibourdenche J,Bedu A,Petzold L,Marchand M,Mariani-Kurdjian P,Hurtaud-Roux MF,Aujard Y,Porquet D

更新日期：2002-03-01 00:00:00

abstract：:An audit of troponin measurement protocols in use in Wales showed significant variations in practice with no consensus on analytical methods, or in the selection of a decision limit for the diagnosis of myocardial damage. Peer review data on assay imprecision at concentrations approaching the analytical limit of detec...

journal_title：Annals of clinical biochemistry

authors： Thomas MA,Singh GK,Williams EJ,McDowell IF,Tovey JA,Wayte AM,All Wales Clinical Biochemistry Audit Group.

更新日期：2005-09-01 00:00:00

abstract：:The number of known inherited metabolic disorders resulting in an organic aciduria has increased steadily over the past two decades. Prompt and reliable detection is both clinically and technically demanding but is essential if appropriate treatment is to be undertaken. This is the first study of laboratory performanc...

journal_title：Annals of clinical biochemistry

authors： Bonham JR,Downing M,Pollitt RJ,Manning NJ,Carpenter KH,Olpin SE,Allen JC,Worthy E

更新日期：1994-03-01 00:00:00

abstract：:Mass spectrometry has for many years enabled us to rapidly identify and quantify many different compounds in biological samples. The equipment is currently available in specialist centres investigating metabolic disorders and in toxicology laboratories. Improvements in sample introduction and refinements in the mass s...

journal_title：Annals of clinical biochemistry

authors： Honour JW

更新日期：2003-11-01 00:00:00

abstract：BACKGROUND:The high-dose hook effect is a well-known phenomenon of two-site immunoassays including those for human chorionic gonadotrophin (hCG). We investigated the occurrence of a high-dose hook effect in six routinely available hCG assays using a sample with a total hCG concentration of approximately 3,600,000 IU/L....

journal_title：Annals of clinical biochemistry

authors： Al-Mahdili HA,Jones GR

更新日期：2010-07-01 00:00:00

abstract：:A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the ...

journal_title：Annals of clinical biochemistry

authors： Brown K,Gardner JJ,Lockley WJ,Preston JR,Wilkinson DJ

更新日期：1983-01-01 00:00:00

abstract：:Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in 50 normal individuals (24 male)...

journal_title：Annals of clinical biochemistry

authors： Haq M,Haq S,Tutt P,Crook M

更新日期：1993-07-01 00:00:00

abstract：:The effect on the concentrations of specific serum proteins and tumour markers, of heat-treating samples at 56 degrees C for 30 min to inactivate HIV, was investigated. Statistically significant decreases, that may affect clinical decisions, were observed for alpha-1-antitrypsin, beta-2-microglobulin, IgE, fibrinogen,...

journal_title：Annals of clinical biochemistry

authors： Gleeson M,Herd L,Burns C

更新日期：1990-11-01 00:00:00

abstract：:Most thyroid-stimulating hormone (TSH) assays now have the sensitivity required by thyroid guidelines and allow the reliable identification of patients with both overt and subclinical hyperthyroidism. Clinical guidelines usually quote decision limits for TSH, but often ignore the issue of whether variability in bias b...

journal_title：Annals of clinical biochemistry

authors： Beckett G,MacKenzie F

更新日期：2007-05-01 00:00:00

abstract：:Background Measurement of serum dexamethasone during the overnight dexamethasone-suppression test has been recommended to reduce false-positive results when investigating Cushing's syndrome or increasingly commonly found adrenal incidentalomas. Despite this, there remains a paucity of well-validated dexamethasone meth...

journal_title：Annals of clinical biochemistry

authors： Hawley JM,Owen LJ,Debono M,Newell-Price J,Keevil BG

更新日期：2018-11-01 00:00:00