Abstract:
BACKGROUND:Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. METHODS:We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, using ornithine-ketoacid aminotransferase, Delta(1)-pyrroline-5-carboxylate dehydrogenase and glutamate dehydrogenase, which together convert citrulline through ornithine to glutamate. The glutamate is then quantitatively measured using glutamate oxidase and Trinder's reagent. RESULTS:The results obtained by this method agreed well with those obtained using the diacetylmonoxime method as a gold standard [correlation coefficient (r) = 0.973 P<0.001]. The endogenous amino acids sensitive to this method in serum (glutamate, ornithine and Delta(1)-pyrroline-5-carboxylate) were eliminated by the initial futile reaction. The new method appears to be more accurate at low levels of ornithine carbamoyltransferase activity than the diacetylmonoxime method. CONCLUSIONS:Here we report a new method for serum ornithine carbamoyl-transferase assay which might be useful for clinical diagnosis of hepatic disorders, including hepatic cancer.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Ishikawa H,Matsuzawa T,Ohashi K,Nagamura Ydoi
10.1258/000456303321610583subject
Has Abstractpub_date
2003-05-01 00:00:00pages
264-8issue
Pt 3eissn
0004-5632issn
1758-1001journal_volume
40pub_type
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