An automated method for the analysis of urinary free catecholamines using ASTED and high-pressure liquid chromatography.


:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs.


Ann Clin Biochem


Green B,Cooper JD,Turnell DC




Has Abstract


1989-07-01 00:00:00








26 ( Pt 4)


  • Prevalence of macroamylasaemia using polyethylene glycol precipitation as a screening method.

    abstract::Four hundred and fifty-four subjects with hyperamylasaemia were screened for the presence of macroamylase using polyethylene glycol precipitation (PEG) and the Beckman automated amylase assay based on the hydrolysis of maltotetraose. Twenty-five subjects (5.5%) exhibited PEG precipitation values suggestive of macroamy...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Lawson GJ

    更新日期:2001-01-01 00:00:00

  • Bhattacharya: a new application for quality control.

    abstract::The Bhattacharya method and the 'average of normals' method for internal quality control were compared. Both are based on unselected patient test results. The Bhattacharya method is mostly used in clinical chemistry for calculation of reference intervals but could be modified for quality control procedures. Using the ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Oosterhuis WP,Modderman TA,Dinkelaar RB,Zwinderman AH,van der Helm HJ

    更新日期:1991-07-01 00:00:00

  • A simple automated colorimetric method for determination of N-acetyl- beta-D-glucosaminidase.

    abstract::An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Moore JC,Morris JE

    更新日期:1982-05-01 00:00:00

  • Identification of an unusual gamma counter fault using Trigg's technique for trend detection.

    abstract::A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Challand GS,Cox L

    更新日期:1978-03-01 00:00:00

  • Information management and informatics: need for a modern pathology service.

    abstract::Requirements for information technology in pathology now extend well beyond the provision of purely analytical data. With the aim of achieving seamless integration of laboratory data into the total clinical pathway, "informatics"--the art and science of turning data into useful information--is becoming increasingly im...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Jones R,O'Connor J

    更新日期:2004-05-01 00:00:00

  • Effect of storage of plasma and serum on enzymatic determination of non-esterified fatty acids.

    abstract::Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated p...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Menéndez LG,Fernández AL,Enguix A,Ciriza C,Amador J

    更新日期:2001-05-01 00:00:00

  • Biochemical investigation of foetal and neonatal thyroid function using the ACS-180SE analyser: clinical application.

    abstract::Despite sonographic detection of foetal goitre, uncertainty persists in the initial diagnosis of thyrotoxicosis and hypothyroidism. The aim of this study was to establish foetal and neonatal iodothyronine and thyrotrophin reference values for the ACS-180SE analyser. From 22 to 36 weeks of gestation, median foetal seru...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Guibourdenche J,Noël M,Chevenne D,Vuillard E,Voluménie JL,Polak M,Boissinot C,Porquet D,Luton D

    更新日期:2001-09-01 00:00:00

  • The influence of age and sex on tests of thyroid function.

    abstract::Serum concentrations of total thyroid hormones, thyroid hormone binding proteins, free thyroid hormones and thyroid stimulating hormone (TSH) were measured in a large group of healthy normal subjects (n = 218) to determine the influence of age and sex on tests of thyroid function. No effect of age on measurements of t...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Franklyn JA,Ramsden DB,Sheppard MC

    更新日期:1985-09-01 00:00:00

  • Urinary free catecholamines--diagnostic application of an HPLC technique to the investigation of neural crest tumours.

    abstract::Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Davidson DF

    更新日期:1987-09-01 00:00:00

  • The influence of a cooked-meat meal on estimated glomerular filtration rate.

    abstract:BACKGROUND:Chronic kidney disease (CKD) is an important but under-recognized condition. Recent national guidelines have recommended that biochemistry laboratories report estimated GFR (eGFR) to improve diagnosis of CKD and facilitate disease staging and management. Previous reports have suggested that intake of large a...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Preiss DJ,Godber IM,Lamb EJ,Dalton RN,Gunn IR

    更新日期:2007-01-01 00:00:00

  • Validity of estimating non-sex hormone-binding globulin bound testosterone and oestradiol from total hormone measurements in boys and girls.

    abstract:BACKGROUND:Circulating levels of bioavailable oestradiol and testosterone are often desirable for clinical practice or investigational studies of children. However, assays to measure circulating hormone levels might not always be accessible. We sought to validate the empirical calculation of circulating bioavailable te...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,随机对照试验


    authors: Egleston BL,Chandler DW,Dorgan JF

    更新日期:2010-05-01 00:00:00

  • Improved continuous-flow analysis for serum bilirubin.

    abstract::A simple and improved method of continuous-flow analysis for total and direct bilirubin in serum is described. Advantages of the method are: small serum and reagent volumes; the use of less concentrated caffeine solution in the accelerator; the substitution of distilled water for hydrochloric acid in the direct biliru...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Lolekha PH

    更新日期:1980-09-01 00:00:00

  • Pregnancy and inherited metabolic disorders: maternal and fetal complications.

    abstract::Some inherited metabolic disorders (IMDs) can cause significant complications during pregnancy, affecting the mother and/or the fetus. Although it appears that only a minority of IMDs have these effects, experience is still being acquired. For some disorders, patients will not have reached child-bearing age. Pregnanci...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审


    authors: Preece MA,Green A

    更新日期:2002-09-01 00:00:00

  • Enzyme immunoassay: a review.

    abstract::In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems involved in preparing enzyme labels and the ways in which such labels can be used in...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,评审


    authors: O'Sullivan MJ,Bridges JW,Marks V

    更新日期:1979-09-01 00:00:00

  • Unusual gamma heavy chain disease protein in a patient with splenic marginal-zone lymphoma.

    abstract::We conducted an electrophoretic analysis of monoclonal gamma-globulin found in the serum of a patient with splenic marginal-zone lymphoma. This monoclonal protein showed electrophoretic mobility to the gamma region and reacted with anti-immunoglobulin (IgG) antiserum but not with anti-kappa or anti-lambda light chain ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Morita K,Kawamoto H,Takada H,Nakamura S,Ishii K,Okamoto Y

    更新日期:2006-03-01 00:00:00

  • Clinical impact of variability in HbA1c as assessed by simultaneously measuring fructosamine and use of error grid analysis.

    abstract:BACKGROUND:Haemoglobin A1c (HbA1c) is the only measure of glycaemic control used for many patients with diabetes, but it has limitations and might sometimes be misleading. HbA(1c) concentrations are influenced by conditions that alter red-cell life and there is evidence that biochemical variation in intracellular glyca...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Macdonald DR,Hanson AM,Holland MR,Singh BM

    更新日期:2008-07-01 00:00:00

  • Recurrent dizzy spells: all in the head!

    abstract::Hypoglycaemia is an uncommon manifestation of hypopituitarism in non-diabetic adults. A 47-year-old woman with Cushing's disease was treated with bilateral adrenalectomy and pituitary radiotherapy following unsuccessful trans-sphenoidal surgery. Twenty years later, she presented with a tonic-clonic seizure and recurre...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: McAulay V,Seckl JR,Strachan MW

    更新日期:2005-01-01 00:00:00

  • A new method for haptoglobin phenotyping.

    abstract::Haptoglobin is an acute-phase protein with three major phenotypes: Hp 1-1, Hp 2-1 and Hp 2-2. Studies have shown that functional differences between these phenotypes have important consequences in a number of pathological disorders (e.g. cardiovascular disease, autoimmune disorders, infectious disease), making phenoty...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Wassell J,Keevil B

    更新日期:1999-09-01 00:00:00

  • Antibodies to glutamic acid decarboxylase in young Chinese diabetic patients.

    abstract::Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Ko GT,Chan JC,Yeung VT,Chow CC,Li JK,Lau MS,Mackay IR,Rowley MJ,Zimmet P,Cockram CS

    更新日期:1998-11-01 00:00:00

  • Influence of glucuronosyl bilirubin and (EZ)-cyclobilirubin on determination of serum unbound bilirubin by UB-analyser.

    abstract:BACKGROUND:In the enzyme reaction for the determination of the unbound (free) bilirubin concentration by glucose oxidase and peroxidase, materials with low affinity for serum protein are reactive. The influence of these materials on the determination of serum unbound bilirubin was investigated. METHODS Serum samples fr...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Itoh S,Kawada K,Kusaka T,Yasuda S,Okada H,Imai T,Isobe K

    更新日期:2002-11-01 00:00:00

  • Lack of association between oxidized LDL-cholesterol concentrations and haptoglobin phenotypes in healthy subjects.

    abstract:BACKGROUND:The haptoglobin (Hp) 2-2 phenotype has been known to have less antioxidative activity than Hp 1-1 and Hp 2-1. However, few have reported the relationship between oxidative stress and Hp phenotypes by using oxidized LDL. The relationship between Hp phenotypes and oxidative stress was therefore investigated in...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Lee YW,Min WK,Chun S,Lee W,Park H,Lee YK,Lee DH,Son JE

    更新日期:2004-11-01 00:00:00

  • Near-patient test for nicotine and its metabolites in saliva to assess smoking habit.

    abstract::Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Cope G,Nayyar P,Holder R,Brock G,Chapple I

    更新日期:2000-09-01 00:00:00

  • Evaluation of the Randox colorimetric serum copper and zinc assays against atomic absorption spectroscopy.

    abstract:BACKGROUND:Analysis of copper and zinc in serum is commonly performed using atomic absorption spectrometry (AAS); however, these methods are often not readily available in smaller laboratories. Randox colorimetric assays for copper and zinc in serum were evaluated on the Thermo Electron Data Pro analyser against flame ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Beckett JM,Hartley TF,Ball MJ

    更新日期:2009-07-01 00:00:00

  • A direct comparison of patient-based real-time quality control techniques: The importance of the analyte distribution.

    abstract:BACKGROUND:Patient-based real-time quality control (PBRTQC) techniques have been described in clinical chemistry for over 50 years. PBRTQC has a number of advantages over traditional quality control including commutability, cost and the opportunity for real-time monitoring. However, there are few systematic investigati...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Smith JD,Badrick T,Bowling F

    更新日期:2020-05-01 00:00:00

  • Variation in phlebotomy techniques in emergency medicine and the incidence of haemolysed samples.

    abstract:BACKGROUND:Phlebotomy is a potential cause of preanalytical errors. We have observed phlebotomy in routine practice in a busy Emergency Department, to see how current practice compares with optimal blood sampling. METHODS:Phlebotomy episodes were audited and compared with standard procedures. A computer-based search o...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Berg JE,Ahee P,Berg JD

    更新日期:2011-11-01 00:00:00

  • Serum iron and total iron-binding capacity determination by flow-injection analysis with atomic absorption detection.

    abstract::The deproteinised sample (150 microliters) is 'injected' into a continuously flowing stream of deionised water which is pumped, via a capillary tube, to the nebuliser of an atomic absorption spectrophotometer. Analytical readout is obtained, in the form of transient peaks, 6 s after sample injection. Before injection ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Rocks BF,Sherwood RA,Turner ZJ,Riley C

    更新日期:1983-03-01 00:00:00

  • A study of high-, middle- and low-molecular weight adiponectin in urine as a surrogate marker for early diabetic nephropathy using ultrasensitive immune complex transfer enzyme immunoassay.

    abstract::Background For the early identification of patients at risk of developing diabetic nephropathy, we have developed an ultrasensitive immune complex transfer enzyme immunoassay to measure adiponectin in urine. Methods We developed immune complex transfer enzyme immunoassay for adiponectin and measured urinary adiponecti...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Yamamoto M,Fujimoto Y,Hayashi S,Hashida S

    更新日期:2018-09-01 00:00:00

  • Superoxide dismutase (erythrocuprein) and free radicals in clinical chemistry.

    abstract::Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Gutteridge JM

    更新日期:1976-05-01 00:00:00

  • Development of a rapid liquid chromatography tandem mass spectrometry method for the quantitation of serum dexamethasone and its clinical verification.

    abstract::Background Measurement of serum dexamethasone during the overnight dexamethasone-suppression test has been recommended to reduce false-positive results when investigating Cushing's syndrome or increasingly commonly found adrenal incidentalomas. Despite this, there remains a paucity of well-validated dexamethasone meth...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Hawley JM,Owen LJ,Debono M,Newell-Price J,Keevil BG

    更新日期:2018-11-01 00:00:00

  • The cardiovascular risk marker asymmetric dimethylarginine is elevated in asymptomatic, untreated HIV-1 infection and correlates with markers of immune activation and disease progression.

    abstract:BACKGROUND:As lifespan in HIV infection increases, cardiovascular disease has emerged as a cause of morbidity and mortality. Asymmetric dimethylarginine is an established marker of endothelial dysfunction and predicts cardiovascular events. The role of asymmetric dimethylarginine in HIV-related cardiovascular disease h...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章


    authors: Hudson CL,Zemlin AE,Ipp H

    更新日期:2014-09-01 00:00:00