Identification of glucagon-like peptide-1(7-36) amide in rat brain.

Abstract:

:Acid-urea extract of rat brain was examined by glucagon-like peptide-1 (GLP-1) specific radioimmunoassay. A single peak was observed which co-eluted with GLP-1(7-36)amide on gel filtration and anion exchange chromatography. In contrast, GLP-1(1-37) was not detected under our experimental conditions. The fact that GLP-1 (7-36)amide, but not GLP-1(1-37), was present in rat brain suggests that preproglucagon was processed in the brain in the same manner as in the intestine and not as in the pancreas.

journal_name

Ann Clin Biochem

authors

Yoshimoto S,Hirota M,Ohboshi C,Shima K

doi

10.1177/000456328902600214

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

169-71

eissn

0004-5632

issn

1758-1001

journal_volume

26 ( Pt 2)

pub_type

杂志文章
  • A modified method for the estimation of galactose-1-phosphate.

    abstract::The measurement of galactose-1-phosphate has been advocated for monitoring galactosaemia. A radioenzymatic method is described in which red-cell haemolysates are incubated with radiolabelled uridine diphospho-glucose and exogenous galactose-1-phosphate uridyl transferase. Labelled glucose-1-phosphate is formed in prop...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328602300313

    authors: Dobbie JA,Holton JB

    更新日期:1986-05-01 00:00:00

  • Red blood cell distribution width and neutrophil to lymphocyte ratio are associated with outcomes of adult subarachnoid haemorrhage patients admitted to intensive care unit.

    abstract::Background Red blood cell distribution width (RDW) and neutrophil to lymphocyte ratio (NLR) have been reported to be associated with outcomes of acute cerebral infarction. However, their prognostic value in patients with subarachnoid haemorrhage (SAH) remains largely unknown. The aim of this study was to investigate t...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563216686623

    authors: Huang YL,Han ZJ,Hu ZD

    更新日期:2017-11-01 00:00:00

  • Urinary free catecholamines--diagnostic application of an HPLC technique to the investigation of neural crest tumours.

    abstract::Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328702400512

    authors: Davidson DF

    更新日期:1987-09-01 00:00:00

  • Variation in phlebotomy techniques in emergency medicine and the incidence of haemolysed samples.

    abstract:BACKGROUND:Phlebotomy is a potential cause of preanalytical errors. We have observed phlebotomy in routine practice in a busy Emergency Department, to see how current practice compares with optimal blood sampling. METHODS:Phlebotomy episodes were audited and compared with standard procedures. A computer-based search o...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/acb.2011.011099

    authors: Berg JE,Ahee P,Berg JD

    更新日期:2011-11-01 00:00:00

  • External quality assessment of serum alpha-fetoprotein assays.

    abstract::Adequate control of performance in immunoassays of maternal serum alpha-fetoprotein is an essential part of screening for neural tube defects. Information derived from external quality assessment schemes may be of limited value in monitoring individual laboratory performance and stresses the need for proper internal q...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328001700611

    authors: Woodhead JS,Kemp HA,Nix AB,Rowlands RJ

    更新日期:1980-11-01 00:00:00

  • Review of investigation and management of severe hyponatraemia in a hospital population.

    abstract::The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329903600204

    authors: Crook MA,Velauthar U,Moran L,Griffiths W

    更新日期:1999-03-01 00:00:00

  • A novel method for measuring serum ornithine carbamoyltransferase.

    abstract:BACKGROUND:Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. METHODS:We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, us...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456303321610583

    authors: Ishikawa H,Matsuzawa T,Ohashi K,Nagamura Y

    更新日期:2003-05-01 00:00:00

  • Carnosine supplementation does not affect serum concentrations of advanced glycation and precursors of lipoxidation end products in autism: a randomized controlled clinical trial.

    abstract:BACKGROUND:Abundant evidence indicate the increased levels of oxidative stress in patients with autism. Advanced glycation end products and advanced lipoxidation end products and their precursors play a major role in increased oxidative stress in numerous metabolic and neurologic diseases. Carnosine is a natural dipept...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章,随机对照试验

    doi:10.1177/0004563218796860

    authors: Ghodsi R,Kheirouri S,Nosrati R

    更新日期:2019-01-01 00:00:00

  • The effect of potassium EDTA on the stability of parathyroid hormone in whole blood.

    abstract:BACKGROUND:The stability of parathyroid hormone (PTH) in whole blood has not been well characterized. Previous studies show EDTA plasma samples to be more stable than serum samples. METHODS:Blood from 17 haemodialysis patients and five healthy volunteers was collected into serum separator tubes (SSTs) and potassium ED...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456307780480927

    authors: English E,McFarlane I,Taylor KP,Halsall DJ

    更新日期:2007-05-01 00:00:00

  • Are biochemistry interpretative comments helpful? Results of a general practitioner and nurse practitioner survey.

    abstract:BACKGROUND:Adding or incorporating clinical interpretative comments on biochemistry results is widespread in UK laboratories; although this consumes considerable human resource, there is still little evidence to suggest that it is either effective or appreciated by our clinical colleagues. I therefore decided to survey...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/acb.2007.007134

    authors: Barlow IM

    更新日期:2008-01-01 00:00:00

  • Late diagnosis of primary hyperoxaluria type III.

    abstract::We report the case of a 78-year-old patient with late diagnosis of hyperoxaluria type III (PH3). He developed renal failure after nephrectomy for clear cell papillary renal carcinoma and complained of recurrent urolithiasis for some 30 years, whose aetiology was never identified. Biochemical laboratory investigations ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563216677101

    authors: Richard E,Blouin JM,Harambat J,Llanas B,Bouchet S,Acquaviva C,de la Faille R

    更新日期:2017-05-01 00:00:00

  • Metformin decreases glycated albumin to glycated haemoglobin ratio in patients with newly diagnosed type 2 diabetes.

    abstract:BACKGROUND:To know whether metformin improves postprandial hyperglycaemia, we examined the effect of metformin on the glycated albumin (GA) to glycated haemoglobin (HbA1c) ratio (GA/HbA1c ratio) in patients with newly diagnosed type 2 diabetes. METHODS:Metformin and lifestyle interventions were initiated in 18 patient...

    journal_title:Annals of clinical biochemistry

    pub_type: 临床试验,杂志文章

    doi:10.1177/0004563214522984

    authors: Sumitani S,Morita S,Deguchi R,Hirai K,Mukai K,Utsu Y,Miki S,Sato B,Nakamura H,Kasayama S,Koga M

    更新日期:2015-01-01 00:00:00

  • Interleukin 6 in cerebrospinal fluid of patients with meningitis is not a useful diagnostic marker in the differential diagnosis of meningitis.

    abstract::We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples cl...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329703400206

    authors: López-Cortés LF,Cruz-Ruiz M,Gómez-Mateos J,Jiménez-Hernández D,Viciana-Fernández P,Jiménez-Mejías E

    更新日期:1997-03-01 00:00:00

  • Effect of plantain on therapeutic drug monitoring of digoxin and thirteen other common drugs.

    abstract:INTRODUCTION:Plantain, a herbal remedy, has been reported to interfere with therapeutic drug monitoring of digoxin. We evaluated three commercially available plantain products for potential interference with therapeutic drug monitoring of digoxin and 13 other common drugs. METHOD:Dry content of plantain capsule or pla...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456306776865214

    authors: Dasgupta A,Davis B,Wells A

    更新日期:2006-05-01 00:00:00

  • A direct comparison of patient-based real-time quality control techniques: The importance of the analyte distribution.

    abstract:BACKGROUND:Patient-based real-time quality control (PBRTQC) techniques have been described in clinical chemistry for over 50 years. PBRTQC has a number of advantages over traditional quality control including commutability, cost and the opportunity for real-time monitoring. However, there are few systematic investigati...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563220902174

    authors: Smith JD,Badrick T,Bowling F

    更新日期:2020-05-01 00:00:00

  • Association of serum omentin-1 concentrations with the presence and severity of preeclampsia.

    abstract:BACKGROUND:Inflammation is involved in the pathogenesis of preeclampsia. Omentin is a newly discovered adipokine that possesses an anti-inflammatory function. The aim of this study was to determine the association of serum omentin-1 concentration with the presence and severity of preeclampsia. METHODS:Serum concentrat...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563214541247

    authors: Liu H,Wu J,Wang H,Sheng L,Tang N,Li Y,Hao T

    更新日期:2015-03-01 00:00:00

  • A method for measuring glycerol-blanked triglyceride concentrations by using gas chromatography-isotope dilution mass spectrometry.

    abstract:BACKGROUND:Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563220921884

    authors: Koyama I,Imano H,Nakamura M,Kitamura A,Kiyama M,Miyamoto Y,Iso H

    更新日期:2020-05-01 00:00:00

  • The reduction of peripheral insulin concentrations in obese subjects following a hypocaloric diet: reduced pancreatic secretion or increased hepatic extraction?

    abstract::The fasting hyperinsulinaemia characteristic of obesity is due to pancreatic hypersecretion and is accompanied by a corresponding increase in plasma C-peptide concentrations. The exaggerated plasma insulin and C-peptide responses to oral glucose in obese subjects which is greater for insulin than C-peptide suggests th...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328902600502

    authors: Gama R,Marks V

    更新日期:1989-09-01 00:00:00

  • 2,8-Dihydroxyadenine renal stones in a 41-year-old man.

    abstract::A case is presented of a 41-year-old man with a history of recurrent renal stones over 10 years. Analysis of the stone showed that, although it gave a positive reaction with the non-specific phosphotungstic acid test, uricase failed to identify any urate present. Analysis in a reference laboratory confirmed its compos...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/000456304322880087

    authors: Wilkinson H,Samuell C,Stower M

    更新日期:2004-03-01 00:00:00

  • Clinical impact of variability in HbA1c as assessed by simultaneously measuring fructosamine and use of error grid analysis.

    abstract:BACKGROUND:Haemoglobin A1c (HbA1c) is the only measure of glycaemic control used for many patients with diabetes, but it has limitations and might sometimes be misleading. HbA(1c) concentrations are influenced by conditions that alter red-cell life and there is evidence that biochemical variation in intracellular glyca...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/acb.2008.007259

    authors: Macdonald DR,Hanson AM,Holland MR,Singh BM

    更新日期:2008-07-01 00:00:00

  • Gas chromatographic screening for drugs and metabolites in plasma and urine.

    abstract::There is an increasing need for sensitive and specific methods for toxicological screening. Development of a dual-column gas chromatography system for the screening of drugs and metabolites in the plasma of poisoned patients is described. The system has been evaluated using plasma from a well-documented series of pati...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328402100203

    authors: Jarvie DR,Simpson D

    更新日期:1984-03-01 00:00:00

  • Comparison of biochemical with radiological findings in renal osteodystrophy.

    abstract::In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456327501200126

    authors: Moorhead JF,Varghese Z,Tatler GL,Baillod RA,Wills MR

    更新日期:1975-05-01 00:00:00

  • Verification of placental growth factor and soluble-fms-like tyrosine kinase 1 assay performance in late pregnancy and their diagnostic test accuracy in women with reduced fetal movement.

    abstract:BACKGROUND:Placental growth factor (PlGF) and soluble-fms-like tyrosine kinase 1 (sFlt-1) are biomarkers of placental function used to aid the diagnosis and prediction of pregnancy complications. This work verified the analytical performance of both biomarkers and provides preliminary diagnostic accuracy data to identi...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563220911993

    authors: Armstrong-Buisseret LK,Haslam S,James T,Bradshaw L,Heazell AE

    更新日期:2020-05-01 00:00:00

  • Seasonal variation in serum 25-hydroxy vitamin D3 does not affect 1,25-dihydroxy vitamin D.

    abstract::The seasonal variation of serum 25-hydroxy vitamin D3 and 1,25-dihydroxy-vitamin D has been investigated. Blood was taken from 27 healthy volunteers, aged 21-44 years old at 3 monthly intervals over a period of 1 year. A scrolling monthly programme with 12 quarterly (3 month) time periods was developed. A summer assoc...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329403100105

    authors: Hine TJ,Roberts NB

    更新日期:1994-01-01 00:00:00

  • A rapid method for measuring serum amyloid A protein by latex agglutination nephelometric immunoassay.

    abstract::Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enric...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329303000112

    authors: Yamada T,Nomata Y,Sugita O,Okada M

    更新日期:1993-01-01 00:00:00

  • Application and validation of a urinary methadone metabolite (EDDP) immunoassay to monitor methadone compliance.

    abstract::A total of 1381 urine specimens were screened using a Microgenics CEDIA urinary primary methadone metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; EDDP) immunoassay (cut-off calibrator concentration of 100 microg/L) in combination with a Dade Behring EMIT urinary methadone immunoassay (cut-off calibrator...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1258/0004563001899267

    authors: George S,Parmar S,Meadway C,Braithwaite RA

    更新日期:2000-05-01 00:00:00

  • Troponin T for the differential diagnosis of ischaemic myocardial damage.

    abstract::The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456329303000102

    authors: Collinson PO,Moseley D,Stubbs PJ,Carter GD

    更新日期:1993-01-01 00:00:00

  • Radioimmunoassay of sodium cromoglycate in human plasma.

    abstract::A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the ...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328302000105

    authors: Brown K,Gardner JJ,Lockley WJ,Preston JR,Wilkinson DJ

    更新日期:1983-01-01 00:00:00

  • Association of serum and vitreous concentrations of osteoprotegerin with diabetic retinopathy.

    abstract:BACKGROUND:Angiogenesis is involved in the pathogenesis of diabetic retinopathy. Osteoprotegerin, a recently identified glycoprotein belonging to the tumour necrosis factor receptor superfamily, has been implicated to be correlated with angiogenesis. This study aims to determine whether serum and vitreous concentration...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/0004563214533669

    authors: Yu G,Ji X,Jin J,Bu S

    更新日期:2015-03-01 00:00:00

  • Screening of bacteria in urine using luciferin-luciferase assay of microbial ATP: a comparative study.

    abstract::A total of 3227 urine specimens were analysed to investigate the applicability of a firefly luciferase assay of microbial adenosine triphosphate (ATP) for the rapid screening of bacteriuria in clinical specimens. Urine sediment, dipslide culture, and three plate cultures were also investigated in the majority of the u...

    journal_title:Annals of clinical biochemistry

    pub_type: 杂志文章

    doi:10.1177/000456328201900605

    authors: Ruokonen A,Koskinen M,Leinonen M,Tiilikainen A,Vihko R

    更新日期:1982-11-01 00:00:00