Abstract:
:Lack of accuracy in expressing concentrations of specific proteins in different lots of Hyland calibrating reference and quality control materials, supplied for the Hyland PDQ laser nephelometer, prompted an attempt to find alternative and more reliable calibrating materials available commercially. Laser grade (Atlantic Antibodies) and radial immunodiffusion grade materials (Behring Diagnostics) were evaluated in this study. Using Atlantic Antibodies' calibrator, only five of 11 specific proteins assayed in Atlantic control serum were within acceptable ranges; precision was less than that using Hyland materials of identical lots. Mean values for each of 10 of the same 11 specific proteins of Behring control plasma assayed against Behring stabilised human serum, were within manufacturers' acceptable ranges; however, precision was less than that with Hyland materials from identical lots. Nine of the 11 specific proteins in a frozen serum pool sample, measured on the laser nephelometer, agreed well with results of the specific proteins measured by radial immunodiffusion, using the same Behring calibration and quality control. Only the Hyland materials were prediluted for use, which explains the greater precision that was obtained. Intercomparison of concentrations, as expressed by respective manufacturers, was largely unacceptable. Based on these results there is need, until satisfactory reagents become available, to standardise on one parent Hyland reference sample giving best precision and to calibrate all subsequent working standards against the parent reference.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Shulman Gdoi
10.1177/000456328001700403subject
Has Abstractpub_date
1980-07-01 00:00:00pages
178-82issue
4eissn
0004-5632issn
1758-1001journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Golgi protein-73 (GP73) is a newly identified candidate serum marker for liver diseases. The utility of this biomarker remains limited, largely due to the lack of quantitative information. The aims of this study were to quantify serum GP73 (sGP73) in healthy individuals and in patients with liver diseases, a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.008088
更新日期:2009-01-01 00:00:00
abstract::Two cases of hypercholesterolaemic patients are presented in whom raised plasma creatine kinase was noted during treatment with statins. The plasma creatine kinase failed to fall following cessation of therapy. Further investigation revealed the aetiology of the raised plasma creatine kinase to be due to previously un...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303322326542
更新日期:2003-09-01 00:00:00
abstract::The presence of antibodies in some patients' serum has long been known to be a potential source of interference in immunoassays, as shown by numerous case reports. These often appear after the introduction of a new analyte (e.g. troponin) and then decrease in number as the topic becomes exhausted. This highlights the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456302760042128
更新日期:2002-07-01 00:00:00
abstract::We report the case of a 78-year-old patient with late diagnosis of hyperoxaluria type III (PH3). He developed renal failure after nephrectomy for clear cell papillary renal carcinoma and complained of recurrent urolithiasis for some 30 years, whose aetiology was never identified. Biochemical laboratory investigations ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563216677101
更新日期:2017-05-01 00:00:00
abstract::Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500609
更新日期:1998-11-01 00:00:00
abstract::We studied the serum protein binding of 3H-labelled progesterone, oestradiol and testosterone, and five 125I-labelled analogues of these steroids. All tracers investigated appeared to be bound by proteins in every serum sample tested. The addition of blocking agents caused a substantial reduction in serum protein bind...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329503200609
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:Recent NICE Guidelines have emphasized the need to have in place by 2007 the capability of offering screening to all women in the first trimester using a combination of maternal age with the ultrasound marker nuchal translucency thickness (NT) and the maternal serum biochemical markers free beta-hCG and preg...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563053026880
更新日期:2005-01-01 00:00:00
abstract::Direct assays have been described for the estimation of progesterone in serum and saliva. Both methods showed good inter-assay precision over a year, and the serum assay is now being used routinely in clinical laboratories. A highly significant correlation was obtained for progesterone concentrations in matched serum ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200605
更新日期:1985-11-01 00:00:00
abstract::We investigated the effect of a fatty meal on plasma concentrations of lipids, apolipoproteins, and the cholesterol component of lipoproteins. Sixteen nonobese, healthy, asymptomatic males, 22-34 years of age, served as subjects for this study. None smoked, consumed more than two alcoholic drinks per day, or took any ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700512
更新日期:1990-09-01 00:00:00
abstract::Plasma creatine kinase (CK) activity and lean body mass (LBM) (by anthropometry) were measured in 120 women (20-82 years) and 70 men (20-82 years). Plasma CK activity and LBM was higher in men than in women. In men plasma CK activity was significantly correlated (product moment correlation coefficient) with LBM and ne...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500411
更新日期:1988-07-01 00:00:00
abstract:BACKGROUND:Interpretative commenting constitutes an important aspect of the post-analytical phase in chemical pathology, but has only recently been the subject of quality assessment. The Royal College of Pathologists of Australasia (RCPA)-Australasian Association of Clinical Biochemists (AACB) Chemical Pathology Patien...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901955
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:We evaluated procalcitonin (PCT) assay in the emergency diagnosis of neonatal bacterial infection, especially in preterm infants, relative to C-reactive protein (CRP) and fibrinogen. METHODS:One hundred and twenty neonates (32 preterm), of whom 21 were infected, were tested. RESULTS:Concentrations of PCT, ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901874
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Current practice requires regular venous blood samples for monitoring of tacrolimus concentrations post renal transplant requiring regular hospital visits. Mitra devices use volumetric absorptive microsampling technology and absorb a fixed amount of blood (10 μL) from a capillary blood sample. They are a via...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220948886
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Pharmacometabonomics is a new branch of science, first described in 2006 and defined as 'the prediction of the effects of a drug on the basis of a mathematical model of pre-dose metabolite profiles'. Pharmacometabonomics has been used to predict drug metabolism, pharmacokinetics (PK), drug safety and drug ef...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563213497929
更新日期:2013-11-01 00:00:00
abstract::The spectral interference caused by haemoglobin in the calcium-cresolphthalein complexone reaction was investigated. The results show that the nature of the haemoglobin derivative in the reaction mixture influences the degree of interference; in turn, the derivative formed depends on the order in which the sample and ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700212
更新日期:1990-03-01 00:00:00
abstract::Free and total reduced concentrations of D-penicillamine have been measured in the plasma of rheumatoid arthritis patients by HPLC and electro-chemical detection. A reverse-phase ion-pair separation in conjunction with a dual porous graphite electrode satisfied the requirements of robustness, sensitivity, selectivity ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500210
更新日期:1988-03-01 00:00:00
abstract::The analysis of serum albumin by bromocresol purple (BCP), by bromocresol green (BCG), and by electroimmunoassay (EIA) has been compared in a mixed group of 100 hospital patients. Good agreement between the immunochemical and BCP methods, but a positive bias with the BCG method led to routine use of the BCP method. In...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200314
更新日期:1985-05-01 00:00:00
abstract::There is no definitive treatment protocol for alkaptonuria. A patient with alkaptonuria was treated with ascorbic acid (0.5 g/day) from the age of 4 years. He developed episodes of severe recurrent joint pain at 9.5 years of age after which a protein-restricted diet (1.3 g/kg/day) was started. Protein restriction in c...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303321016268
更新日期:2003-01-01 00:00:00
abstract::The effects of inhibition of inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in guanine nucleotide de novo synthesis, on cell growth, cell viability, endogenous nucleotide concentrations and concentrations of extracellular nucleosides and bases were studied in Molt F4 human malignant lymphoblasts...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329403100211
更新日期:1994-03-01 00:00:00
abstract:BACKGROUND:Angiogenesis is involved in the pathogenesis of diabetic retinopathy. Osteoprotegerin, a recently identified glycoprotein belonging to the tumour necrosis factor receptor superfamily, has been implicated to be correlated with angiogenesis. This study aims to determine whether serum and vitreous concentration...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214533669
更新日期:2015-03-01 00:00:00
abstract::We examined the reproducibility of oral glucose tolerance tests (OGTT) using the World Health Organization criterion in 212 Chinese subjects (male 149, female 63) who underwent two 75 g OGTTs within a 6-week period. The overall reproducibility was 65.6% (139/212) of which 74 subjects had normal glucose tolerance, 24 h...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500107
更新日期:1998-01-01 00:00:00
abstract::A patient with sepsis and jaundice was admitted for diagnosis and treatment. Associated biochemical changes included increased C-reactive protein, conjugated bilirubin and gamma-glutamyltransferase, the duration of which was protracted. High urine coproporphyrin isomer-1 and immunostaining of liver tissue suggested Du...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2012.011279
更新日期:2012-11-01 00:00:00
abstract::A radioimmoassay for digoxin is described which uses commercially available reagents. Two assay procedures are used, one for batch assays, and another, which needs a 5 minute incubation only, for 'stat' assays. The methods are shown to be simple, rapid, precise and inexpensive and the batch procedure compared well wit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200216
更新日期:1985-03-01 00:00:00
abstract::Mitochondrial acetoacetyl-CoA thiolase deficiency (or beta-ketothiolase deficiency) is a rare metabolic disorder characterized by acute episodes of severe acidosis and ketosis. A case is presented of an 18-month-old boy who presented with vomiting and diarrhoea and was found to be markedly acidotic. When the acidosis ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.007202
更新日期:2008-09-01 00:00:00
abstract::The activity of serum deoxycytidylate deaminase, not hitherto reported to increase after myocardial infarction, has been investigated and the results have been related to other cardiac enzymes, including the MB fraction of creatine phosphokinase. Deoxycytidylate deaminase was found to increase to maximum activity on t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327801500175
更新日期:1978-11-01 00:00:00
abstract:BACKGROUND:Haemoglobin A1c (HbA1c) is the only measure of glycaemic control used for many patients with diabetes, but it has limitations and might sometimes be misleading. HbA(1c) concentrations are influenced by conditions that alter red-cell life and there is evidence that biochemical variation in intracellular glyca...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.007259
更新日期:2008-07-01 00:00:00
abstract::Whole blood and washed erythrocytes from nutritionally normal and abnormal elderly patients were stored at room temperature, 4 degrees C and -20 degrees C for up to 14 days. In both groups, and under all storage conditions, the erythrocyte transketolase (ETK) activity and the thiamine pyrophosphate (TPP) effect decrea...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200417
更新日期:1985-07-01 00:00:00
abstract::Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899717
更新日期:2000-09-01 00:00:00
abstract::This paper reviews the physiology and pathology of oxygen transport from the atmosphere to the cells in critically ill patients. A thorough understanding of this allows a rational approach to monitoring and managing an hypoxic patient in the intensive care setting. ...
journal_title:Annals of clinical biochemistry
pub_type: 指南,杂志文章,评审
doi:10.1177/000456329102800104
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND AND AIM:Familial hypercholesterolaemia is caused by variants in the low-density lipoprotein cholesterol metabolic pathway involving LDLR, APOB and PCSK9 genes. A national genetic testing service in Wales, UK has observed that no familial hypercholesterolaemia variant is found in almost 80% patients with the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218793165
更新日期:2019-01-01 00:00:00