Albumin analysis in serum of haemodialysis patients: discrepancies between bromocresol purple, bromocresol green and electroimmunoassay.

abstract：:This study was undertaken to investigate the extent of the reported discrepancy between serum and plasma albumin concentrations measured by the bromocresol purple (BCP) method. We have shown that plasma albumin concentrations are falsely increased by turbidity due to the precipitation of fibrinogen when plasma is dilu...

journal_title：Annals of clinical biochemistry

authors： Hill PG,Wells TN

更新日期：1983-09-01 00:00:00

abstract：:A radioimmoassay for digoxin is described which uses commercially available reagents. Two assay procedures are used, one for batch assays, and another, which needs a 5 minute incubation only, for 'stat' assays. The methods are shown to be simple, rapid, precise and inexpensive and the batch procedure compared well wit...

journal_title：Annals of clinical biochemistry

authors： Moore RA,Turner M,Cousins J,Gales M

更新日期：1985-03-01 00:00:00

abstract：:Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware of the effect that these technological advances have, both on the analytical and the clinical requirements for provision o...

journal_title：Annals of clinical biochemistry

authors： Sturgeon CM,McAllister EJ

更新日期：1998-07-01 00:00:00

abstract：BACKGROUND:Neutrophilic polymorphonuclear leukocytes play a crucial role in the host defence against bacterial and fungal infections. They participate in the inflammatory response through the liberation of peptides and enzymes like myeloperoxidase (MPO). Therefore, MPO has a potential as a marker enzyme for the diagnos...

journal_title：Annals of clinical biochemistry

authors： Hasmann A,Wehrschuetz-Sigl E,Marold A,Wiesbauer H,Schoeftner R,Gewessler U,Kandelbauer A,Schiffer D,Schneider KP,Binder B,Schintler M,Guebitz GM

更新日期：2013-05-01 00:00:00

abstract：:The diagnostic performance of a new enzyme linked immunosorbent assay for the cardiac structural protein troponin T in the differential diagnosis of ischaemic cardiac damage was assessed. A well documented set of patients admitted to the coronary care unit of a district general hospital were studied. At a cutoff value...

journal_title：Annals of clinical biochemistry

authors： Collinson PO,Moseley D,Stubbs PJ,Carter GD

更新日期：1993-01-01 00:00:00

abstract：:A previously described method for mannitol in urine has been modified and improved. End product inhibition by fructose in the mannitol dehydrogenase method for mannitol has been minimized; the assay is linear over a sample mannitol concentration range of 0-12 mmol/L; no significant interference from other sugars or su...

journal_title：Annals of clinical biochemistry

authors： Blood J,Ingle AR,Allison N,Davies GR,Hill PG

更新日期：1991-07-01 00:00:00

abstract：:The osmolality of plasma and heparinised whole blood samples collected from hospital patients was estimated using measurement of the depression of freezing point. There was no clinically significant difference between osmolality measurement made on either whole blood, or plasma taken from the same patient. Neither cel...

journal_title：Annals of clinical biochemistry

authors： Rocks BF,Sherwood RA,Cook JG

更新日期：1986-01-01 00:00:00

abstract：:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...

journal_title：Annals of clinical biochemistry

authors： Green B,Cooper JD,Turnell DC

更新日期：1989-07-01 00:00:00

abstract：:Background After adrenalectomy, urinary fractionated metanephrine concentrations are expected to be reduced. However, there are few studies suggesting cut-offs for adrenalectomy patients. Methods Urinary metanephrine and normetanephrine concentrations in adrenalectomy patients and two controls were compared and hormon...

journal_title：Annals of clinical biochemistry

authors： Cho YY,Kim YN,Kim JH,Jeong BC,Lee SY,Kim JH

更新日期：2017-01-01 00:00:00

abstract：:The measurement of carbamylated haemoglobin is a useful indicator of uraemic state during the preceding few weeks in patients with renal failure. In diabetic uraemic patients with hyperglycaemia, glycation of haemoglobin may interfere with its carbamylation, as both reactions involve the free amino groups of the prote...

journal_title：Annals of clinical biochemistry

authors： Hammouda AM,Mady GE

更新日期：2001-03-01 00:00:00

abstract：:The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...

journal_title：Annals of clinical biochemistry

authors： Trewick AL

更新日期：1998-03-01 00:00:00

abstract：:We assayed interleukin 6 (IL-6) concentrations in cerebrospinal fluid (CSF) from patients affected by meningitis of different aetiologies, and verified whether IL-6 can be used as a diagnostic marker in the differential diagnosis of meningitis. We used a monoclonal antibody enzyme immunoassay to test 98 CSF samples cl...

journal_title：Annals of clinical biochemistry

authors： López-Cortés LF,Cruz-Ruiz M,Gómez-Mateos J,Jiménez-Hernández D,Viciana-Fernández P,Jiménez-Mejías E

更新日期：1997-03-01 00:00:00

abstract：:A simple and improved method of continuous-flow analysis for total and direct bilirubin in serum is described. Advantages of the method are: small serum and reagent volumes; the use of less concentrated caffeine solution in the accelerator; the substitution of distilled water for hydrochloric acid in the direct biliru...

journal_title：Annals of clinical biochemistry

authors： Lolekha PH

更新日期：1980-09-01 00:00:00

abstract：:Background Measured (MO) and calculated osmotic concentrations (CO) and the osmotic gap (OG) are commonly used in the investigation of electrolyte and volume disturbances as well as in cases of suspected volatile ingestion. Methods We compared 38 published formulae for CO with MO on a large data-set ( n = 9466) and ad...

journal_title：Annals of clinical biochemistry

authors： Wilgen U,Pretorius CJ,Ungerer JP

更新日期：2018-01-01 00:00:00

abstract：:The System 1 produced results for glucose and urea which were precise and accurate. It proved suitable as a general laboratory instrument as it samples 28 mul of specimen, is able to calibrate itself, produces results in three minutes, and has the capacity to analyse 56 samples an hour. ...

journal_title：Annals of clinical biochemistry

authors： Potezny N,Quelch J,Fuller B,Geary TD

更新日期：1977-09-01 00:00:00

abstract：BACKGROUND:The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the ...

journal_title：Annals of clinical biochemistry

authors： McEneny J,McPherson PA,McGinty A,Hull SS,McCance DR,Young IS

更新日期：2013-01-01 00:00:00

abstract：:Guaiac-based faecal occult blood tests (gFOBT) are still used in asymptomatic population bowel screening programmes but are being replaced by faecal immunochemical tests (FIT) for haemoglobin. gFOBT have many well-documented disadvantages and there is little evidence for their use in assessment of the symptomatic. Man...

journal_title：Annals of clinical biochemistry

authors： Fraser CG

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Since more than 90% of cortisol is bound to protein, serum free cortisol (SFC) may be a more appropriate marker of adrenal status than total cortisol. However, measurement of SFC is technically difficult and calculated SFC may offer a more practical alternative. METHODS:SFC, measured by equilibrium dialysis...

journal_title：Annals of clinical biochemistry

authors： Barlow NL,Holme J,Stockley RA,Clark PM

更新日期：2010-05-01 00:00:00

abstract：INTRODUCTION:Plantain, a herbal remedy, has been reported to interfere with therapeutic drug monitoring of digoxin. We evaluated three commercially available plantain products for potential interference with therapeutic drug monitoring of digoxin and 13 other common drugs. METHOD:Dry content of plantain capsule or pla...

journal_title：Annals of clinical biochemistry

authors： Dasgupta A,Davis B,Wells A

更新日期：2006-05-01 00:00:00

abstract：BACKGROUND:With the recent outbreak in West Africa, hospitals worldwide have been developing protocols for suspect of cases of Ebola virus disease. Patients with Ebola virus disease present with a severe gastroenteritis leading to dehydration and electrolyte abnormalities and as such, routine chemistry analysis is esse...

journal_title：Annals of clinical biochemistry

authors： LeBlanc JJ,Heinstein C,MacDonald J,Gallant R,Roberts C,Jackson C,Lou A,Nassar BA,Hatchette TF

更新日期：2016-03-01 00:00:00

abstract：:The most widely used methods for the assay of total bilirubin in serum are based on a coupling reaction with various diazo dyes in the presence of an accelerating agent. The 'direct' reaction, without accelerator, gives a good estimate of the conjugated and protein bound (delta) species of bilirubin only if carefully ...

journal_title：Annals of clinical biochemistry

authors： Westwood A

更新日期：1991-03-01 00:00:00

abstract：:Many attempt are now being made by external agencies to assess the quality of assay performance in hormone laboratories. Some of the problems associated with this are discussed and some remedies are proposed. ...

journal_title：Annals of clinical biochemistry

authors： Jeffcoate SL

更新日期：1981-01-01 00:00:00

abstract：:This paper critically examines the usefulness of serum albumin measurement in the light of current laboratory practice and knowledge of the pathophysiology of albumin metabolism. The main conclusions and recommendations are as follows: (i) Albumin measurement forms a limited, but useful part of the investigation of li...

journal_title：Annals of clinical biochemistry

authors： Whicher J,Spence C

更新日期：1987-11-01 00:00:00

abstract：:The effects of inhibition of inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in guanine nucleotide de novo synthesis, on cell growth, cell viability, endogenous nucleotide concentrations and concentrations of extracellular nucleosides and bases were studied in Molt F4 human malignant lymphoblasts...

journal_title：Annals of clinical biochemistry

authors： Stet EH,De Abreu RA,Bökkerink JP,Lambooy LH,Vogels-Mentink TM,Keizer-Garritsen JJ,Trijbels FJ

更新日期：1994-03-01 00:00:00

abstract：:The application of a DC plasma emission spectrometer to the measurement of copper, zinc and magnesium in serum and urine is reported. Each assay requires only a simple dilution in 1% nitric acid. Comparisons with standard atomic absorption techniques showed good analytical agreement, especially for copper and magnesiu...

journal_title：Annals of clinical biochemistry

authors： Roberts NB,Fairclough D,McLoughlin S,Taylor WH

更新日期：1985-09-01 00:00:00

abstract：:We hereby describe the rare case of a 59-year-old patient presenting with marked hyperamylasaemia mimicking acute pancreatitis upon admission. Investigation of co-existent hypokalemia revealed the presence of ectopic adrenocorticotropic hormone secretion, leading to the final diagnosis of small cell lung cancer, exhib...

journal_title：Annals of clinical biochemistry

authors： Akinosoglou K,Siagris D,Geropoulou E,Kosmopoulou O,Velissaris D,Kyriazopoulou V,Gogos C

更新日期：2014-01-01 00:00:00

abstract：:We developed a new simple assay for potassium ion in serum using urea amidolyase (UAL) from yeast sp. The method is based on activation of the enzyme by potassium ion. We eliminated endogenous ammonium ion by use of glutamate dehydrogenase (GLDH), and then monitored the production of ammonium ion by UAL, urea, ATP, bi...

journal_title：Annals of clinical biochemistry

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Fushimi R,Amino N

更新日期：1997-07-01 00:00:00

abstract：:Background Proglucagon-derived hormones represent a family of peptides mainly produced in the pancreas and the intestine. While several proglucagon-derived peptides play key roles in metabolic diseases, little is known about glicentin. The aim of the present study was to investigate serum glicentin concentrations in i...

journal_title：Annals of clinical biochemistry

authors： Raffort J,Panaïa-Ferrari P,Lareyre F,Blois M,Bayer P,Staccini P,Fénichel P,Chinetti G

更新日期：2018-03-01 00:00:00

abstract：:This review describes the development and application of a novel test to determine levels of human immunoreactive trypsin, an enzyme produced solely by the pancreas, in biological fluids. Being organ-specific, the assay of immunoreactive trypsin should be an ideal marker of pancreatic function, and this is supported b...

journal_title：Annals of clinical biochemistry

authors： Stagg BH,Wood TP

更新日期：1979-05-01 00:00:00

abstract：BACKGROUND:Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB...

journal_title：Annals of clinical biochemistry

authors： Koyama I,Imano H,Nakamura M,Kitamura A,Kiyama M,Miyamoto Y,Iso H

更新日期：2020-05-01 00:00:00