Abstract:
:This study was undertaken to investigate the extent of the reported discrepancy between serum and plasma albumin concentrations measured by the bromocresol purple (BCP) method. We have shown that plasma albumin concentrations are falsely increased by turbidity due to the precipitation of fibrinogen when plasma is diluted into the BCP reagent. Modification of the BCP buffer (to 0.1M acetate containing 0.15M NaCl, pH 5.5) eliminates the turbidity and thus permits the measurement of albumin in either serum or plasma. Plasma albumin concentrations measured with the modified BCP reagent show excellent correlation with results by an immunoturbidimetric method.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Hill PG,Wells TNdoi
10.1177/000456328302000503subject
Has Abstractpub_date
1983-09-01 00:00:00pages
264-70eissn
0004-5632issn
1758-1001journal_volume
20 (Pt 5)pub_type
杂志文章abstract::A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200102
更新日期:1975-01-01 00:00:00
abstract::Erythrocyte and fasting plasma magnesium were assayed by a dyebinding colorimetric method using the metallochromic dye, Calmagite, in 51 diabetic Nigerians and 25 controls. Erythrocyte magnesium concentrations were similar in the diabetics and the controls. Fasting plasma magnesium concentrations were significantly lo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600111
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:To know whether metformin improves postprandial hyperglycaemia, we examined the effect of metformin on the glycated albumin (GA) to glycated haemoglobin (HbA1c) ratio (GA/HbA1c ratio) in patients with newly diagnosed type 2 diabetes. METHODS:Metformin and lifestyle interventions were initiated in 18 patient...
journal_title:Annals of clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1177/0004563214522984
更新日期:2015-01-01 00:00:00
abstract::The Myolex (Orion) and the RapiTex (Behringwerke) latex agglutination tests for the rapid detection of elevated levels of serum myoglobin were studied prospectively in patients suspected of acute myocardial infarction, who were admitted to hospital within 8 h of pain onset. Using admission blood samples drawn 3.4 +/- ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600309
更新日期:1989-05-01 00:00:00
abstract::It is increasingly easy for the general public to access a wide range of laboratory tests. Tests can be ordered online with little or no input from a health professional. The complementary and alternative medicine (CAM) community promote and sell a wide range of tests, many of which are of dubious clinical significanc...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563218824622
更新日期:2019-05-01 00:00:00
abstract::This review describes the development and application of a novel test to determine levels of human immunoreactive trypsin, an enzyme produced solely by the pancreas, in biological fluids. Being organ-specific, the assay of immunoreactive trypsin should be an ideal marker of pancreatic function, and this is supported b...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327901600131
更新日期:1979-05-01 00:00:00
abstract::Four hundred and fifty-four subjects with hyperamylasaemia were screened for the presence of macroamylase using polyethylene glycol precipitation (PEG) and the Beckman automated amylase assay based on the hydrolysis of maltotetraose. Twenty-five subjects (5.5%) exhibited PEG precipitation values suggestive of macroamy...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563011900263
更新日期:2001-01-01 00:00:00
abstract::Background After adrenalectomy, urinary fractionated metanephrine concentrations are expected to be reduced. However, there are few studies suggesting cut-offs for adrenalectomy patients. Methods Urinary metanephrine and normetanephrine concentrations in adrenalectomy patients and two controls were compared and hormon...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215620822
更新日期:2017-01-01 00:00:00
abstract::The calculation of within-laboratory imprecision in quality-assessment (QA) programmes normally involves combining data from different analyte concentrations to calculate an average standard deviation (SD) or coefficient of variation. However, for immunoassay neither of these parameters is concentration independent. T...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328402100611
更新日期:1984-11-01 00:00:00
abstract::A comparative evaluation of Beckman (A), Boehringer (B), and Calbiochem (C) kits for the enzymatic end-point determination of urea has been carried out. Acceptable within-day and day-to-day precision was obtained with all kits. The recoveries obtained fulfilled the criteria of Logan (CRC Critical Reviews in Clinical L...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327801500163
更新日期:1978-09-01 00:00:00
abstract::Whole blood and washed erythrocytes from nutritionally normal and abnormal elderly patients were stored at room temperature, 4 degrees C and -20 degrees C for up to 14 days. In both groups, and under all storage conditions, the erythrocyte transketolase (ETK) activity and the thiamine pyrophosphate (TPP) effect decrea...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200417
更新日期:1985-07-01 00:00:00
abstract:BACKGROUND:Angiogenesis is involved in the pathogenesis of diabetic retinopathy. Osteoprotegerin, a recently identified glycoprotein belonging to the tumour necrosis factor receptor superfamily, has been implicated to be correlated with angiogenesis. This study aims to determine whether serum and vitreous concentration...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214533669
更新日期:2015-03-01 00:00:00
abstract::The spectral interference caused by haemoglobin in the calcium-cresolphthalein complexone reaction was investigated. The results show that the nature of the haemoglobin derivative in the reaction mixture influences the degree of interference; in turn, the derivative formed depends on the order in which the sample and ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329002700212
更新日期:1990-03-01 00:00:00
abstract::Digoxin requesting patterns were studied prospectively over a 6-month period. Clinicians were not forewarned that the study was being undertaken. Many samples received in the laboratory were taken at incorrect times after the drug had been given. This led to inappropriate action being taken in some cases. Appropriate ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328702400410
更新日期:1987-07-01 00:00:00
abstract:BACKGROUND:The worldwide epidemic of obesity is a major public health concern and is persuasively linked to the rising prevalence of diabetes and cardiovascular disease. Obesity is often associated with an abnormal lipoprotein profile, which may be partly negated by pioglitazone intervention, as this can influence the ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1258/acb.2012.012019
更新日期:2013-01-01 00:00:00
abstract:INTRODUCTION:Plantain, a herbal remedy, has been reported to interfere with therapeutic drug monitoring of digoxin. We evaluated three commercially available plantain products for potential interference with therapeutic drug monitoring of digoxin and 13 other common drugs. METHOD:Dry content of plantain capsule or pla...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306776865214
更新日期:2006-05-01 00:00:00
abstract::The measurement of galactose-1-phosphate has been advocated for monitoring galactosaemia. A radioenzymatic method is described in which red-cell haemolysates are incubated with radiolabelled uridine diphospho-glucose and exogenous galactose-1-phosphate uridyl transferase. Labelled glucose-1-phosphate is formed in prop...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300313
更新日期:1986-05-01 00:00:00
abstract::Background The clinical utility of serum thyroglobulin in the follow-up of patients with differentiated thyroid carcinoma may be compromised by the presence of endogenous antithyroglobulin antibodies. To prevent interference by antithyroglobulin antibodies several groups have developed real-time PCR-based assays for q...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563216671538
更新日期:2017-09-01 00:00:00
abstract::Measurement of urine-free (unconjugated) cortisol (UFC) excretion is widely used in the investigation of hypercortisolaemia. We have measured 24 h UFC excretion in normal healthy individuals using a radioimmunoassay (RIA) method claimed to be suitable for unextracted urine. Significantly higher rates of excretion were...
journal_title:Annals of clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1177/000456329403100505
更新日期:1994-09-01 00:00:00
abstract::In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200126
更新日期:1975-05-01 00:00:00
abstract:BACKGROUND:Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a popula...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218811865
更新日期:2019-01-01 00:00:00
abstract::The measurement of glycated haemoglobin (glycated Hb) by automated high pressure liquid chromatography enabled the prevalence of haemoglobin variants (Hb variant) to be determined in a large diabetic population within Leeds, UK. There were 16 patients showing a Hb variant amongst a total of 5300 diabetics, the majorit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900410
更新日期:1992-07-01 00:00:00
abstract::A radioimmoassay for digoxin is described which uses commercially available reagents. Two assay procedures are used, one for batch assays, and another, which needs a 5 minute incubation only, for 'stat' assays. The methods are shown to be simple, rapid, precise and inexpensive and the batch procedure compared well wit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200216
更新日期:1985-03-01 00:00:00
abstract:BACKGROUND:Neutrophilic polymorphonuclear leukocytes play a crucial role in the host defence against bacterial and fungal infections. They participate in the inflammatory response through the liberation of peptides and enzymes like myeloperoxidase (MPO). Therefore, MPO has a potential as a marker enzyme for the diagnos...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2011.010249
更新日期:2013-05-01 00:00:00
abstract::The current search for new treatments to combat coronary heart disease (CHD) is centred on increasing HDL-cholesterol. The failure of the CETP inhibitor torcetrapib may force a rethink. This perspective briefly reviews the antiatherosclerotic properties of HDL and ways HDL-cholesterol concentration can be raised, but ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2008.008133
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:It is now established that the bioactive lipid lysophosphatidic acid (LPA) contributes to cancer initiation, progression and metastasis, including those of prostate cancer. LPA is produced in the serum and plasma mainly by conversion from lysophospholipids through the action of lysophospholipase D (lysoPLD),...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307782268147
更新日期:2007-11-01 00:00:00
abstract::Immunoreactive melatonin has been studied in normal human volunteers, both men and women, at different times of day and different times of the year. At 2400, but not at 0800 or 1200, there are very large inter-individual variations in mean levels, the maximum difference being fivefold. Intra-individual variations are ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327901600182
更新日期:1979-11-01 00:00:00
abstract::Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327501200135
更新日期:1975-07-01 00:00:00
abstract:BACKGROUND:A convenient method for the measurement of sialic acid in plasma apoB-containing lipoproteins is described. METHODS:Dextran sulphate-Mg(2+) precipitation and enzymatic sialic acid assay were combined and applied to analysis of plasma from 96 healthy controls and 136 hyperlipidaemic subjects of types IIa (n=...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563054255614
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Individuals who are unable to metabolize the short-acting muscle relaxant succinylcholine due to abnormal cholinesterase activity are currently investigated via spectrophotometry using artificial substrates and enzyme inhibitors. Methods have been described using succinylcholine as substrate but with measure...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.009169
更新日期:2010-05-01 00:00:00