Abstract:
BACKGROUND:Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB measurements have not been standardized. Therefore, we developed an efficient quantification system for total glycerides and free glycerol that allows the calculation of TG-GB concentrations. METHODS:We measured total glycerides and free glycerol in human serum by using gas chromatography-isotope dilution mass spectrometry and compared its performance to the reference method of the US Centers for Disease Control and Prevention (CDC). RESULTS:Our practical method of total glycerides and free glycerol quantification achieved excellent precision for both within-run and among-run coefficients of variation (<1.5% and <2.7%, respectively), with an average recovery of 99.8% for free glycerol. However, we noted an average %bias of -0.26% for total glycerides and -3.15% for free glycerol between our TG-GB method and the CDC reference method. CONCLUSIONS:This practical method of total glycerides and free glycerol quantification enables traceability assessment of TG-GB measurements. Differences between the output values of TG-GB and the CDC reference method might result from the differences in free glycerol values.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Koyama I,Imano H,Nakamura M,Kitamura A,Kiyama M,Miyamoto Y,Iso Hdoi
10.1177/0004563220921884subject
Has Abstractpub_date
2020-05-01 00:00:00pages
253-261issue
3eissn
0004-5632issn
1758-1001journal_volume
57pub_type
杂志文章abstract::Free and total reduced concentrations of D-penicillamine have been measured in the plasma of rheumatoid arthritis patients by HPLC and electro-chemical detection. A reverse-phase ion-pair separation in conjunction with a dual porous graphite electrode satisfied the requirements of robustness, sensitivity, selectivity ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500210
更新日期:1988-03-01 00:00:00
abstract::An automated assay for serum amylase is described. Using the AutoAnalyzer II the method employs, as substrate, a solution of dyed amylopectin which is commercially available (DyAmyl-L). After incubation of serum and substrate at 55 degrees C, 1.0 mol/lKOH is introduced and the coloured products of enzymic hydrolysis a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327701400190
更新日期:1977-11-01 00:00:00
abstract::Measurement of urinary free cortisol using the Bayer Automated Chemiluminescent System (ACS:180 PLUS) was evaluated and compared with an in-house extraction radioimmunoassay (RIA). Inter-assay coefficients of variation were acceptable, being respectively 5.3%, 4.8% and 3.8% at 141, 406 and 942 nmol/L (n=20) for the AC...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456320003700414
更新日期:2000-07-01 00:00:00
abstract::Many attempt are now being made by external agencies to assess the quality of assay performance in hormone laboratories. Some of the problems associated with this are discussed and some remedies are proposed. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328101800101
更新日期:1981-01-01 00:00:00
abstract::Using a high-performance liquid chromatography method, we measured seven commonly prescribed benzodiazepines (chlordiazepoxide, nitrazepam, nordiazepam, oxazepam, lorazepam, temazepam and diazepam) in 100 urine samples obtained from patients attending the Leeds Addiction Unit. All of the urines selected for investigat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400110
更新日期:1997-01-01 00:00:00
abstract::Clinical biochemists have long known the analytical and clinical limitations of creatinine and creatinine clearance measurement in the assessment of glomerular filtration rate (GFR). This background is reviewed in the article before assessing the utility of cystatin C, the most promising replacement biochemical marker...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/0004563021901847
更新日期:2002-03-01 00:00:00
abstract::Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000407
更新日期:1983-07-01 00:00:00
abstract:OBJECTIVE:The menstrual cycle-related changes in clinical laboratory values were analysed by use of data obtained in the Asian multicentre study aimed at derivation of common reference intervals for 85 major clinical laboratory tests. METHODS:Among 1876 healthy female volunteers, 893 had regular menstruation. They wer...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215617212
更新日期:2016-05-01 00:00:00
abstract::Most UK clinical laboratories use alkaline phosphatase (ALP) methods similar to that proposed by the International Federation of Clinical Chemistry (IFCC), based on the use of 2-amino-2-methyl-1-propanol (AMP) buffer. We present evidence of significant differences in results produced by apparently similar commercial A...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500117
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND:Refeeding syndrome (RS) is a potentially fatal condition that can occur following the re-introduction of nutrition after a period of starvation. Hypophosphataemia following the reintroduction of nutrition is often the only reliable biochemical marker of RS. Refeeding index (RI) generated from baseline insuli...
journal_title:Annals of clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1177/0004563214523739
更新日期:2015-01-01 00:00:00
abstract::The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500212
更新日期:1998-03-01 00:00:00
abstract:BACKGROUND:Circulating levels of bioavailable oestradiol and testosterone are often desirable for clinical practice or investigational studies of children. However, assays to measure circulating hormone levels might not always be accessible. We sought to validate the empirical calculation of circulating bioavailable te...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1258/acb.2010.009112
更新日期:2010-05-01 00:00:00
abstract::There is no definitive treatment protocol for alkaptonuria. A patient with alkaptonuria was treated with ascorbic acid (0.5 g/day) from the age of 4 years. He developed episodes of severe recurrent joint pain at 9.5 years of age after which a protein-restricted diet (1.3 g/kg/day) was started. Protein restriction in c...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303321016268
更新日期:2003-01-01 00:00:00
abstract::The standard colorimetric methods most often used for the measurement of serum unsaturated iron binding capacity (UIBC) are subject to gross interference by iron dextran. This paper describes a brief evaluation of an alternative radiometric assay for serum UIBC, based on a commercially available kit method, but incorp...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500323
更新日期:1988-05-01 00:00:00
abstract::This paper reviews the physiology and pathology of oxygen transport from the atmosphere to the cells in critically ill patients. A thorough understanding of this allows a rational approach to monitoring and managing an hypoxic patient in the intensive care setting. ...
journal_title:Annals of clinical biochemistry
pub_type: 指南,杂志文章,评审
doi:10.1177/000456329102800104
更新日期:1991-01-01 00:00:00
abstract::Background Measurement of serum dexamethasone during the overnight dexamethasone-suppression test has been recommended to reduce false-positive results when investigating Cushing's syndrome or increasingly commonly found adrenal incidentalomas. Despite this, there remains a paucity of well-validated dexamethasone meth...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218766566
更新日期:2018-11-01 00:00:00
abstract::γ-glutamyltransferase plays a key role in the synthesis and metabolism of extracellular glutathione, a major antioxidant in several defence mechanisms in the body. γ-glutamyltransferase is affected by environmental and genetic factors, and is raised when there is depletion of glutathione. Hence, it is a marker of oxid...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563215597010
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563053492739
更新日期:2005-03-01 00:00:00
abstract::A real time data processing system for a group of three clinical chemistry laboratories (one base and two satellite) has been developed as a successor to one used previously. This paper describes both the inadequacies of the previous worksheet-based system and the advantages of the new system, including flexibility, f...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327901600105
更新日期:1979-01-01 00:00:00
abstract::This paper describes some factors to which attention should be paid when atrial natriuretic peptide (ANP) values are to be interpreted. Changing from the supine to the sitting position decreased ANP levels, while no further decrease was observed when the upright position was assumed. On the other hand, plasma renin ac...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600604
更新日期:1989-11-01 00:00:00
abstract::A radioimmoassay for digoxin is described which uses commercially available reagents. Two assay procedures are used, one for batch assays, and another, which needs a 5 minute incubation only, for 'stat' assays. The methods are shown to be simple, rapid, precise and inexpensive and the batch procedure compared well wit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200216
更新日期:1985-03-01 00:00:00
abstract::Protoporphyrin and zinc-protoporphyrin were measured in the erythrocytes of normal subjects, workers exposed to lead and patients with iron deficiency and erythropoietic protoporphyria (EPP). Results showed significantly higher levels of zinc-protoporphyrin in the lead-exposed workers (P < 0.0001), patients with iron ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500313
更新日期:1998-05-01 00:00:00
abstract::Hypogonadism in the male results from inadequate testicular function, especially defects in androgen synthesis and secretion, or action. Androgen action is important throughout normal male development: in the fetus, puberty, adult life and old age. Regulation is by variable activity of the hypothalamo-pituitary axis a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2010.010150
更新日期:2010-11-01 00:00:00
abstract::Gestational diabetes is a common pregnancy disorder which is generally managed with diet, exercise, metformin or insulin treatment and which usually resolves after delivery of the infant. Identifying and treating gestational diabetes improves maternal and fetal outcomes and allows for health promotion to reduce the mo...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/0004563216674743
更新日期:2017-01-01 00:00:00
abstract::A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600412
更新日期:1989-07-01 00:00:00
abstract::The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500507
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Chronic kidney disease (CKD) is an important but under-recognized condition. Recent national guidelines have recommended that biochemistry laboratories report estimated GFR (eGFR) to improve diagnosis of CKD and facilitate disease staging and management. Previous reports have suggested that intake of large a...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307779595995
更新日期:2007-01-01 00:00:00
abstract::A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protecte...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300410
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:Ischaemia-modified albumin (IMA) is a relatively new marker of ischaemia. IMA has not been studied previously in pleural effusions due to congestive heart failure (CHF). The aim of our study was to assess the diagnostic value of IMA in the pleural fluid (PF) and serum for the identification of pleural effusi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.010159
更新日期:2011-01-01 00:00:00
abstract::Homocysteine, a sulphur amino acid, is a branch-point intermediate of methionine metabolism. It can be degraded in the transsulphuration pathway to cystathionine, or remethylated to methionine via the remethylation pathway. In both pathways, major genetic defects that cause enzyme deficiencies are associated with very...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456303321016169
更新日期:2003-01-01 00:00:00