Near-patient test for nicotine and its metabolites in saliva to assess smoking habit.

Abstract:

:Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure nicotine and its metabolites by a colorimetric assay. The device can be used to give a semi-quantitative assessment of tobacco consumption by observing a colour change and comparing it to a reference chart. Alternatively, the test can be quantified by measuring the light absorbance with a colorimeter and determining the concentration of nicotine metabolites by reference to a cotinine standard. This method correlates with reported daily cigarette consumption (r=0.62, P<0.0001) and nicotine yield of cigarettes smoked (r=0.35, P<0.01) and compares favourably with cotinine, as measured independently by radioimmunoassay (r=0.57, P<0.005). The method provides a simple-to-use, inexpensive way of monitoring tobacco consumption in dental surgeries.

journal_name

Ann Clin Biochem

authors

Cope G,Nayyar P,Holder R,Brock G,Chapple I

doi

10.1258/0004563001899717

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

666-73

eissn

0004-5632

issn

1758-1001

journal_volume

37 ( Pt 5)

pub_type

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