Abstract:
:Three sensitive assays (Behring, Organon and LKB-Pharmacia) for measurement of serum thyrotropin (TSH) were evaluated. All three assays showed good precision, sensitivity, linearity and for practical purposes negligible high dose hook effects. The correlations between the assays were excellent, but due to standardisation and/or matrix effects there were incomplete recoveries in two kits and the kits showed systematic differences of up to 20% in the TSH values obtained. The clinical utility of the three assays was investigated in patients with thyroidal and non-thyroidal illnesses and in healthy subjects with thyroxine binding abnormalities. According to the results of the analytical and clinical evaluation, all three kits are acceptable and reliable as first-line thyroid function tests.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Bonte HA,Vermes I,v d Sluijs Veer Gdoi
10.1177/000456328902600609subject
Has Abstractpub_date
1989-11-01 00:00:00pages
508-16eissn
0004-5632issn
1758-1001journal_volume
26 ( Pt 6)pub_type
杂志文章abstract::The higher bias of serum cystine estimations by a HPLC method compared with those by ion exchange techniques is shown to be largely due to differences in the sample preparation procedures of the two techniques. The ion exchange methods utilised sulphosalicylic acid serum protein precipitation and post-column ninhydrin...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500516
更新日期:1988-09-01 00:00:00
abstract::Background We investigated the in vitro effects of various phospholipids as emulsifiers on the hydrolysing activities of lipoprotein lipase (LPL) Arg243His against triolein as substrate. LPL Arg243His, identified in a patient with hyperchylomicronaemia, displays severely diminished activity for triolein when emulsifie...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217693258
更新日期:2017-11-01 00:00:00
abstract::Addison's disease is a relatively common disorder to endocrinologists, but is rare and potentially fatal when presenting acutely. Treatment now involves replacement of glucocorticoids and mineralocorticoids with synthetic compounds, although historically patients took common salt and plant-based preparations. We descr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780945624
更新日期:2007-07-01 00:00:00
abstract::There is no definitive treatment protocol for alkaptonuria. A patient with alkaptonuria was treated with ascorbic acid (0.5 g/day) from the age of 4 years. He developed episodes of severe recurrent joint pain at 9.5 years of age after which a protein-restricted diet (1.3 g/kg/day) was started. Protein restriction in c...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303321016268
更新日期:2003-01-01 00:00:00
abstract::Serum concentrations of total thyroid hormones, thyroid hormone binding proteins, free thyroid hormones and thyroid stimulating hormone (TSH) were measured in a large group of healthy normal subjects (n = 218) to determine the influence of age and sex on tests of thyroid function. No effect of age on measurements of t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200506
更新日期:1985-09-01 00:00:00
abstract::We examined the reproducibility of oral glucose tolerance tests (OGTT) using the World Health Organization criterion in 212 Chinese subjects (male 149, female 63) who underwent two 75 g OGTTs within a 6-week period. The overall reproducibility was 65.6% (139/212) of which 74 subjects had normal glucose tolerance, 24 h...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500107
更新日期:1998-01-01 00:00:00
abstract::The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329903600204
更新日期:1999-03-01 00:00:00
abstract::The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500212
更新日期:1998-03-01 00:00:00
abstract::Hypoglycaemia is an uncommon manifestation of hypopituitarism in non-diabetic adults. A 47-year-old woman with Cushing's disease was treated with bilateral adrenalectomy and pituitary radiotherapy following unsuccessful trans-sphenoidal surgery. Twenty years later, she presented with a tonic-clonic seizure and recurre...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Plasma C1 inhibitor (C1-INH) was determined in 64 patients with different malignant diseases and in 58 healthy persons. C1-INH antigen concentration was measured by radial immunodiffusion (RID), whereas its functional activity was assayed with chromogenic substrate. Within-run and day-to-day precision of both methods ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800609
更新日期:1991-11-01 00:00:00
abstract::We examined the reactivity of human serum albumin-bound bilirubin and its photoisomers as substrates for a direct bilirubin assay using bilirubin oxidase. The reduction of (EZ)-cyclobilirubin reached 100% 5 min after addition of the enzyme at any pH tested (3.5-7.4) in 0.1 mol/L phosphate buffer, whereas the reduction...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456320003700404
更新日期:2000-07-01 00:00:00
abstract::Rabbit uterus contains type I and II oestrogen binding sites in the cytosol, and nuclear fractions. Oestrogen-stimulated increase in uterine weight was inhibited by concurrent treatment with progesterone or danazol. Oestrogen-induced type I binding sites were decreased by progesterone and to a lesser extent by danazol...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800309
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563053492739
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:We evaluated the diagnostic discrimination of a new micro-flow cell device (Nanoduct) which measures sweat conductivity in situ at a regional referral centre for cystic fibrosis (CF). METHODS:The device was evaluated in comparison to the measurement of sweat chloride with the established quantitative piloca...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306778520025
更新日期:2006-09-01 00:00:00
abstract::A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327801500126
更新日期:1978-03-01 00:00:00
abstract::Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899717
更新日期:2000-09-01 00:00:00
abstract::Heterophilic antibodies are well described, but poorly appreciated interferents and is often not a recognized problem affecting most immunoassays. We report a patient presented with ectopic Cushing's syndrome (CS), but repeated plasma adrenocorticotropic hormone (ACTH) concentrations conducted by immunoassay were inap...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563214520751
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Sickle cell disease is a heterogenous disorder characterized by an abnormal haemoglobin and sickling phenomena of red cells. It is prevalent among certain nomadic tribes in Sudan. Painful, aplastic and haemolytic crises mark sickle cell anaemia. Haemoglobin S (HbS) is detected using haemoglobin electrophores...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2008.008155
更新日期:2009-03-01 00:00:00
abstract::Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000407
更新日期:1983-07-01 00:00:00
abstract:BACKGROUND:The relationship between plasma angiopoietin-like protein 3 (ANGPTL3), and lipoprotein lipase (LPL) activity and hepatic triglyceride lipase (HTGL) activity has not been investigated in the metabolism of remnant lipoproteins (RLPs) and high-density lipoprotein (HDL) in human plasma. METHODS:ANGPTL3, LPL act...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2010.009307
更新日期:2010-09-01 00:00:00
abstract::Using a high-performance liquid chromatography method, we measured seven commonly prescribed benzodiazepines (chlordiazepoxide, nitrazepam, nordiazepam, oxazepam, lorazepam, temazepam and diazepam) in 100 urine samples obtained from patients attending the Leeds Addiction Unit. All of the urines selected for investigat...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400110
更新日期:1997-01-01 00:00:00
abstract::Iron status is influenced by inflammation when the normal control of iron metabolism is reorganized by the primary mediators of the acute phase response, tumour necrosis factor-alpha and interleukin-1. The objective of this review is to show how indices of iron status, particularly haemoglobin, serum ferritin and solu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2007.007167
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a popula...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563218811865
更新日期:2019-01-01 00:00:00
abstract::Insulin resistance is a common condition, recognized to be a central feature of the metabolic syndrome, and strongly associated with an increased risk of cardiovascular disease and diabetes. The quantitative assessment of insulin sensitivity is not used for routine clinical purposes, but the emerging importance of ins...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/000456307780945778
更新日期:2007-07-01 00:00:00
abstract::Auto-antibodies to oxidized low-density lipoprotein (ox-LDL) are thought to play a pivotal role in the pathogenesis of atherosclerosis. This study investigates the value of auto-antibodies to ox-LDL as a predictive marker of atherosclerosis in patients with both homozygous and heterozygous familial hypercholesterolaem...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899177
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:About 10 years ago, a survey showed significant deficiencies in the quality of gastrointestinal investigations performed by UK laboratories. Subsequently, several clinical guidelines have been developed and external quality assessment schemes introduced. This survey was performed to assess whether earlier de...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307780117911
更新日期:2007-03-01 00:00:00
abstract::A real time data processing system for a group of three clinical chemistry laboratories (one base and two satellite) has been developed as a successor to one used previously. This paper describes both the inadequacies of the previous worksheet-based system and the advantages of the new system, including flexibility, f...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327901600105
更新日期:1979-01-01 00:00:00
abstract::The hypoglycaemia caused by non-islet cell tumours is often associated with an increase in plasma insulin-like activity. In many cases there is a relative if not always absolute increase in plasma insulin-like growth factor II (IGF-II). Growth hormone (GH) secretion is almost invariably depressed as is the plasma insu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900312
更新日期:1992-05-01 00:00:00
abstract::A simple and improved method of continuous-flow analysis for total and direct bilirubin in serum is described. Advantages of the method are: small serum and reagent volumes; the use of less concentrated caffeine solution in the accelerator; the substitution of distilled water for hydrochloric acid in the direct biliru...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328001700509
更新日期:1980-09-01 00:00:00
abstract::The effect of progestins on intracellular corticosteroid-binding globulin (CBG) mRNA expression in an endometrial cancer cell line (Ishikawa) was examined in an attempt to understand the biological effects of high-dose progestins in the treatment of well-differentiated uterine endometrial cancers. Oestradiol-17 beta (...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329803500507
更新日期:1998-09-01 00:00:00