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Evaluation of the particle gel diffusion technique for the detection of haemoglobin S in Sudanese patients.

Abstract：

BACKGROUND:Sickle cell disease is a heterogenous disorder characterized by an abnormal haemoglobin and sickling phenomena of red cells. It is prevalent among certain nomadic tribes in Sudan. Painful, aplastic and haemolytic crises mark sickle cell anaemia. Haemoglobin S (HbS) is detected using haemoglobin electrophoresis, iso-electric focusing and/or high-performance liquid chromatography techniques with high sensitivity, but entails cost and expertise. This study aimed to determine the sensitivity, specificity and positive predictive values (PPV) of the ID-particle gel diffusion technique for screening, diagnosis and phenotyping of HbS in patients with a provisional diagnosis of abnormal haemoglobin. METHODS:Following informed consent, 100 sequential individuals who reported to a central referral haemoglobinopathy clinic were enrolled. ID-particle gel diffusion technique was compared with cellulose acetate electrophoresis to determine haemoglobin phenotypes. RESULTS:The ID-particle gel test detected HbAA with 100% sensitivity and 100% specificity. Sensitivity for HbS was 100%, whether as HbSS or as a mixed pattern. HbSS was identified in all cases where this is the only haemoglobin present. Other patterns were detected with <100% specificity and these would require further testing by other means to definitively identify abnormal haemoglobins. CONCLUSIONS:Although the ID-particle technique is a simple and cheap technique with high sensitivity, specificity and PPV compared with cellulose acetate electrophoresis in detecting HbSS, it could not differentiate HbAS from HbSS with high levels of HbF. High environmental temperatures could melt the test microtubes. Cellulose acetate electrophoresis remains the technique of choice for the screening of abnormal haemoglobins in the Sudan.

journal_name

Ann Clin Biochem

journal_title

Annals of clinical biochemistry

authors

Nimir AA,Khalil EA,Musa AM,Yassin HM,El-Hassan AM

doi

10.1258/acb.2008.008155

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

114-6

issue

Pt 2

eissn

0004-5632

issn

1758-1001

pii

acb.2008.008155

journal_volume

pub_type

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Evaluation of the particle gel diffusion technique for the detection of haemoglobin S in Sudanese patients.