Comparison of three kits for the enzymatic determination of urea.

abstract：:Hereditary renal hypouricaemia (HRH) is a genetic disorder commonly associated with exercise-induced acute kidney injury (AKI). We report the case of a 19-year-old man who was admitted to hospital with exercise-induced AKI and who was subsequently shown to have HRH. We believe this to be the first description of a cas...

journal_title：Annals of clinical biochemistry

authors： Chakraborty S,Sural S

更新日期：2013-05-01 00:00:00

abstract：:A simple HPLC method for the determination of caffeine and theophylline in plasma is described. Separation of theobromine, paraxanthine, theophylline, beta-hydroxyethyltheophylline and caffeine is obtained using a mobile phase of 1% acetic acid/methanol (83:17, v/v) and a Waters Associates NOVA-PAK C18 column protecte...

journal_title：Annals of clinical biochemistry

authors： Pickard CE,Stewart AD,Hartley R,Lucock MD

更新日期：1986-07-01 00:00:00

abstract：:An investigation into the use of bilirubin oxidase to measure the apparent concentration of unbound bilirubin in solutions of bilirubin or bilirubin/human serum albumin demonstrated (1) near linearity of oxidation rate with bilirubin concentration up to 16 mumol/L, (2) linearity with respect to enzyme concentration up...

journal_title：Annals of clinical biochemistry

authors： Goldfinch ME,Maguire GA

更新日期：1988-01-01 00:00:00

abstract：:Background The aim of the present study is to evaluate the utility of extraction versus non-extraction-based commercial melatonin ELISA kits for determining the melatonin concentration in amniotic fluid obtained in early and late pregnancy. Methods Pregnancy duration less than 28 weeks was defined as early and from 28...

journal_title：Annals of clinical biochemistry

authors： Bagci S,Altuntas Ö,Katzer D,Berg C,Willruth A,Reutter H,Bartmann P,Müller A,Zur B

更新日期：2017-01-01 00:00:00

abstract：:Mitochondrial acetoacetyl-CoA thiolase deficiency (or beta-ketothiolase deficiency) is a rare metabolic disorder characterized by acute episodes of severe acidosis and ketosis. A case is presented of an 18-month-old boy who presented with vomiting and diarrhoea and was found to be markedly acidotic. When the acidosis ...

journal_title：Annals of clinical biochemistry

authors： Tilbrook LK,Slater J,Agarwal A,Cyriac J

更新日期：2008-09-01 00:00:00

abstract：:We studied the serum protein binding of 3H-labelled progesterone, oestradiol and testosterone, and five 125I-labelled analogues of these steroids. All tracers investigated appeared to be bound by proteins in every serum sample tested. The addition of blocking agents caused a substantial reduction in serum protein bind...

journal_title：Annals of clinical biochemistry

authors： Micallef JV,Hayes MM,Latif A,Ahsan R,Sufi SB

更新日期：1995-11-01 00:00:00

abstract：BACKGROUND:The pneumatic tube system (PTS) has been implicated in inducing haemolysis. It is not known whether certain sample types are more susceptible to haemolysis than others. We assessed the level of haemolysis in commonly used sample types in the clinical biochemistry department when transported through the PTS. ...

journal_title：Annals of clinical biochemistry

authors： Sodi R,Darn SM,Stott A

更新日期：2004-05-01 00:00:00

abstract：:Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...

journal_title：Annals of clinical biochemistry

authors： Gutteridge JM

更新日期：1976-05-01 00:00:00

abstract：BACKGROUND:Recent NICE Guidelines have emphasized the need to have in place by 2007 the capability of offering screening to all women in the first trimester using a combination of maternal age with the ultrasound marker nuchal translucency thickness (NT) and the maternal serum biochemical markers free beta-hCG and preg...

journal_title：Annals of clinical biochemistry

authors： Spencer K

更新日期：2005-01-01 00:00:00

abstract：:Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...

journal_title：Annals of clinical biochemistry

authors： Reynolds TM

更新日期：1997-05-01 00:00:00

abstract：:We hereby describe the rare case of a 59-year-old patient presenting with marked hyperamylasaemia mimicking acute pancreatitis upon admission. Investigation of co-existent hypokalemia revealed the presence of ectopic adrenocorticotropic hormone secretion, leading to the final diagnosis of small cell lung cancer, exhib...

journal_title：Annals of clinical biochemistry

authors： Akinosoglou K,Siagris D,Geropoulou E,Kosmopoulou O,Velissaris D,Kyriazopoulou V,Gogos C

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:We evaluated the recently released chemiluminescence assay for 25-hydroxy vitamin D (25-OHD) on the Immunodiagnostic Systems iSYS (IDS-iSYS) automated analyser. METHODS:The IDS-iSYS comparison was performed using patient samples previously measured for 25-OHD by a validated liquid chromatography-tandem mass...

journal_title：Annals of clinical biochemistry

authors： Cluse ZN,Fudge AN,Whiting MJ,McWhinney B,Parkinson I,O'Loughlin PD

更新日期：2012-03-01 00:00:00

abstract：:A high-pressure liquid chromatography procedure for the measurement of free catecholamines in urine is described. 'On-line' sample preparation using a cartesian robot combined with the ASTED process ensures minimal operator intervention and reduced costs. ...

journal_title：Annals of clinical biochemistry

authors： Green B,Cooper JD,Turnell DC

更新日期：1989-07-01 00:00:00

abstract：:Many contradictory results have been published on the stability of total non-esterified fatty acids in blood, plasma and serum under different storage conditions. The present study was undertaken to investigate the stability of non-esterified fatty acids, measured with an enzymatic method, in samples of EDTA-treated p...

journal_title：Annals of clinical biochemistry

authors： Menéndez LG,Fernández AL,Enguix A,Ciriza C,Amador J

更新日期：2001-05-01 00:00:00

abstract：:Eleven unfit bank executives in sedentary employment exercised daily for 10 weeks according to the Canadian Air-Force 5BX Exercise Programme. Over this period no changes were observed in the glucose or insulin responses to a standard breakfast or to intravenous glucose, or in the glucose response to intravenous insuli...

journal_title：Annals of clinical biochemistry

authors： Moore RA,Penfold WA,Simpson RD,Simpson RW,Mann JI,Turner RC

更新日期：1979-03-01 00:00:00

abstract：:Ethylene glycol in plasma, urine or dialysis fluid is analysed as the phenylboronate derivative by mixing with acetonitrile/acidified 2,2-dimethoxypropane containing phenylboronic acid. After centrifugation, a portion of the supernatant is analysed directly by gas-liquid chromatography using a 3% OV-101 column at 150 ...

journal_title：Annals of clinical biochemistry

authors： Flanagan RJ,Dawling S,Buckley BM

更新日期：1987-01-01 00:00:00

abstract：:Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...

journal_title：Annals of clinical biochemistry

authors： Phillips IJ,Rowe DJ,Dewar P,Connett GJ

更新日期：1999-11-01 00:00:00

abstract：:The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...

journal_title：Annals of clinical biochemistry

authors： Simkins A,Worth HG

更新日期：1976-01-01 00:00:00

abstract：:Most UK clinical laboratories use alkaline phosphatase (ALP) methods similar to that proposed by the International Federation of Clinical Chemistry (IFCC), based on the use of 2-amino-2-methyl-1-propanol (AMP) buffer. We present evidence of significant differences in results produced by apparently similar commercial A...

journal_title：Annals of clinical biochemistry

authors： Lamb EJ,Browne M,John WG,Price CP

更新日期：1998-01-01 00:00:00

abstract：:A case is presented of a 41-year-old man with a history of recurrent renal stones over 10 years. Analysis of the stone showed that, although it gave a positive reaction with the non-specific phosphotungstic acid test, uricase failed to identify any urate present. Analysis in a reference laboratory confirmed its compos...

journal_title：Annals of clinical biochemistry

authors： Wilkinson H,Samuell C,Stower M

更新日期：2004-03-01 00:00:00

abstract：:A total of 1381 urine specimens were screened using a Microgenics CEDIA urinary primary methadone metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine; EDDP) immunoassay (cut-off calibrator concentration of 100 microg/L) in combination with a Dade Behring EMIT urinary methadone immunoassay (cut-off calibrator...

journal_title：Annals of clinical biochemistry

authors： George S,Parmar S,Meadway C,Braithwaite RA

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:It has been suggested that for the accurate measurement of calcium in urine, samples must be collected into bottles containing acid. Acidification poses risks to both patients and laboratory staff. Here we reappraise whether acidification is a preanalytical necessity. METHODS:Twenty-four-hour urine samples ...

journal_title：Annals of clinical biochemistry

authors： Sodi R,Bailey LB,Glaysher J,Allars L,Roberts NB,Marks EM,Fraser WD

更新日期：2009-11-01 00:00:00

abstract：BACKGROUND:Biological variation of an analyte is an important determinant of its usefulness. Intraindividual variation in serum thyroid hormones, parathyroid hormone (PTH) and insulin-like growth factor-1 (IGF-1) in healthy subjects was determined in this study. METHODS:Blood samples were taken at weekly intervals for...

journal_title：Annals of clinical biochemistry

authors： Ankrah-Tetteh T,Wijeratne S,Swaminathan R

更新日期：2008-03-01 00:00:00

abstract：:The purpose of this study was to assess retrospectively the prevalence of severe hyponatraemia in a hospital population and its laboratory investigation, treatment and clinical outcome. Over a 6-month period 47 patients (27 women and 20 men) were found to have a plasma sodium concentration of < or = 120 mmol/L (this n...

journal_title：Annals of clinical biochemistry

authors： Crook MA,Velauthar U,Moran L,Griffiths W

更新日期：1999-03-01 00:00:00

abstract：BACKGROUND:The analysis of steroids in biological matrices is challenging. One can apply immunoassay as well as gas and liquid chromatography with various types of detection, depending on the available equipment and the experience of the analyst. The question is how the methods are interchangeable between themselves. D...

journal_title：Annals of clinical biochemistry

authors： Kosicka K,Siemiątkowska A,Szpera-Goździewicz A,Krzyścin M,Bręborowicz G,Główka F

更新日期：2019-01-01 00:00:00

abstract：:A total of 3227 urine specimens were analysed to investigate the applicability of a firefly luciferase assay of microbial adenosine triphosphate (ATP) for the rapid screening of bacteriuria in clinical specimens. Urine sediment, dipslide culture, and three plate cultures were also investigated in the majority of the u...

journal_title：Annals of clinical biochemistry

authors： Ruokonen A,Koskinen M,Leinonen M,Tiilikainen A,Vihko R

更新日期：1982-11-01 00:00:00

abstract：:Digoxin requesting patterns were studied prospectively over a 6-month period. Clinicians were not forewarned that the study was being undertaken. Many samples received in the laboratory were taken at incorrect times after the drug had been given. This led to inappropriate action being taken in some cases. Appropriate ...

journal_title：Annals of clinical biochemistry

authors： O'Donnell JG,Paterson NC,Pledger DR,Stewart MJ

更新日期：1987-07-01 00:00:00

abstract：BACKGROUND:Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a popula...

journal_title：Annals of clinical biochemistry

authors： Thompson MJ,Parameswaran V,Burgess JR

更新日期：2019-01-01 00:00:00

abstract：:A group of international experts prepared two lists of drugs with their serum/plasma and urine concentrations, which should be used when evaluating the performance of a new laboratory method. The two lists were verified by running in vitro interference studies in three European laboratories on Hitachi instruments. The...

journal_title：Annals of clinical biochemistry

authors： Sonntag O,Scholer A

更新日期：2001-07-01 00:00:00

abstract：:A rapid and simple method for detecting circulating immune complexes based on turbidity measurements following polyethylene glycol precipitation was studied with regard to its suitability as a routine assay in clinical laboratories. This method was found to have an acceptable degree of precision provided the temperatu...

journal_title：Annals of clinical biochemistry

authors： Kilpatrick DC,Weston J,Irvine WJ

更新日期：1981-11-01 00:00:00