Abstract:
BACKGROUND:We evaluated the recently released chemiluminescence assay for 25-hydroxy vitamin D (25-OHD) on the Immunodiagnostic Systems iSYS (IDS-iSYS) automated analyser. METHODS:The IDS-iSYS comparison was performed using patient samples previously measured for 25-OHD by a validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method (n = 119) and an IDS enzyme immunoassy (IDS-EIA) method (n = 64). Limit of detection and limit of quantification were determined from a precision profile. Imprecision was assessed using quality control material and pooled serum. External QAP material (Vitamin D External Quality Assessment Scheme, UK) was analysed to establish inaccuracy. Linearity was assessed by two dilution studies. Cross-reactivity was determined by three serial dilution studies of patient samples with known 25-OHD(2) concentrations. RESULTS:The IDS-iSYS correlated well with both established methods (iSYS = 1.03LC-MS/MS - 6.53, R(2) = 0.82 and iSYS = 1.07IDS-EIA - 1.61, R(2) = 0.86). Imprecision of the iSYS assay for IDS control material was 13.4% at 32 nmol/L, 10% at 78 nmol/L, 9.4% at 161 nmol/L, and for the pooled material 9.3% at 72 nmol/L and 5.6% at 158 nmol/L. The evaluation found the assay to be highly accurate (IDS-iSYS = 0.93ALTM + 3.79, R(2) = 0.94) and linear (obs(1) = 0.93exp(1) - 5.05, R(2) = 0.99 (P = 0.256); and obs(2) = 0.97exp(2) + 6.07, R(2) = 0.97 (P = 0.654); ALTM, all-laboratory trimmed mean). Cross-reactivity studies demonstrated no significant difference to the calculated total 25-OHD as measured by LC-MS/MS. CONCLUSIONS:Even though the imprecision of the iSYS was found to be greater than that of the LC-MS/MS and EIA methods, the performance characteristics of the IDS-iSYS 25-OHD assay are suitable for routine diagnostic purposes on a high throughput automated analyser.
journal_name
Ann Clin Biochemjournal_title
Annals of clinical biochemistryauthors
Cluse ZN,Fudge AN,Whiting MJ,McWhinney B,Parkinson I,O'Loughlin PDdoi
10.1258/acb.2011.011018subject
Has Abstractpub_date
2012-03-01 00:00:00pages
159-65issue
Pt 2eissn
0004-5632issn
1758-1001pii
acb.2011.011018journal_volume
49pub_type
杂志文章abstract::Rabbit uterus contains type I and II oestrogen binding sites in the cytosol, and nuclear fractions. Oestrogen-stimulated increase in uterine weight was inhibited by concurrent treatment with progesterone or danazol. Oestrogen-induced type I binding sites were decreased by progesterone and to a lesser extent by danazol...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800309
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Serum ornithine carbamoyltransferase is a diagnostic marker of hepatic disorders due to its localization in periportal mitochondria. METHODS:We have developed a new method for the determination of serum ornithine carbamoyltransferase. It is based on the reverse reaction of ornithine carbamoyltransferase, us...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456303321610583
更新日期:2003-05-01 00:00:00
abstract::Further data suggesting that assessment of atypicality may be a valuable addition to Down's syndrome screening is presented, based on results from 1521 unaffected pregnancies and 190 chromosomally abnormal pregnancies. For a 1% increase in false positive rates (over the Down's screening-related false positives), it is...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329703400314
更新日期:1997-05-01 00:00:00
abstract::Circulating insulin-like growth factor-1 (IGF-1) is increasingly being used as a screening test and in ongoing monitoring of treated acromegaly. We here present three cases of women (two of whom were on the oestrogen containing contraceptive pill at the time of presentation) who had normal circulating IGF-1 and no ove...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456307779596075
更新日期:2007-01-01 00:00:00
abstract::Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328302000407
更新日期:1983-07-01 00:00:00
abstract::Tiaprofenic acid is a non-steroidal anti-inflammatory drug which also has hypouriceamic effect. Studies involving 10 healthy volunteers were designed to investigate the mode of this effect. We postulate that the site of action of tiaprofenic acid is at the cell membrane, the mechanism being an interference with the tr...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500601
更新日期:1988-11-01 00:00:00
abstract:BACKGROUND:Serum triglyceride concentrations are measured as total glycerides content in many Western countries. In Japan, glycerol-blanked triglycerides (TG-GB) are measured to identify postprandial hypertriglyceridaemia and to minimize the influence of glycerol formulation on serum triglyceride values. However, TG-GB...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563220921884
更新日期:2020-05-01 00:00:00
abstract::We have made an evaluation of glycosylated haemoglobin measurements by affinity chromatography in a paediatric population. Age-related reference values for non-diabetic infants and children are reported. A poor correlation (r = 0.24) was found between glycosylated haemoglobin values in 26 insulin-dependent diabetic ch...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328602300407
更新日期:1986-07-01 00:00:00
abstract::The analytical and biological components of variation of total cholesterol, high density lipoprotein (HDL) cholesterol, HDL3 cholesterol, HDL2 cholesterol and apolipoproteins A-I and B in serum were assessed in 12 apparently healthy individuals. All analytes showed marked individuality and therefore conventional popul...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600314
更新日期:1989-05-01 00:00:00
abstract::The activity of serum deoxycytidylate deaminase, not hitherto reported to increase after myocardial infarction, has been investigated and the results have been related to other cardiac enzymes, including the MB fraction of creatine phosphokinase. Deoxycytidylate deaminase was found to increase to maximum activity on t...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327801500175
更新日期:1978-11-01 00:00:00
abstract:BACKGROUND:Apolipoprotein concentrations in reflex tears from healthy and noninsulin-dependent diabetes mellitus (NIDDM) subjects were measured and correlated with the stage of diabetic retinopathy. METHODS AND RESULTS:The apolipoprotein A-I (apo A-I) concentrations in the tears from NIDDM patients with retinopathy we...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021901748
更新日期:2002-01-01 00:00:00
abstract::In a 1 year prospective study we evaluated the effect of feedback of laboratory data on the requesting behaviour of physicians in general medicine. Data on within-hours and out-of-hours clinical chemistry laboratory usage and revenue expenditure for inpatients and outpatients, expressed in terms of clinical workload, ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329102800204
更新日期:1991-03-01 00:00:00
abstract::The fasting hyperinsulinaemia characteristic of obesity is due to pancreatic hypersecretion and is accompanied by a corresponding increase in plasma C-peptide concentrations. The exaggerated plasma insulin and C-peptide responses to oral glucose in obese subjects which is greater for insulin than C-peptide suggests th...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328902600502
更新日期:1989-09-01 00:00:00
abstract::Serum retinol-binding protein (RBP), with a biological half-life of less than 12 h, is a useful indicator of liver or kidney dysfunction. An automated immunoturbidimetric assay for the measurement of RBP has been developed using the Cobas-BIO centrifugal analyser and commercially available materials. Serum samples wit...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328802500309
更新日期:1988-05-01 00:00:00
abstract::The immunosuppressive agent mycophenolate mofetil is a prodrug for the active compound mycophenolic acid. Following organ transplantation, it is used mostly in combination with other immunosuppressive drugs, but it has also been used as primary therapy. Although in the pivotal clinical studies the drug was given in fi...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563021902035
更新日期:2002-05-01 00:00:00
abstract::The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456327601300105
更新日期:1976-01-01 00:00:00
abstract:OBJECTIVE:The menstrual cycle-related changes in clinical laboratory values were analysed by use of data obtained in the Asian multicentre study aimed at derivation of common reference intervals for 85 major clinical laboratory tests. METHODS:Among 1876 healthy female volunteers, 893 had regular menstruation. They wer...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563215617212
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Glycated haemoglobin (HbA1c) concentration is lower relative to glyacemic control in diabetic patients on haemodialysis. However, it is unknown as to whether this is also true for diabetic patients with end-stage renal disease but not on haemodialysis. METHODS:Correlations between HbA1c or glycated albumin ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/acb.2011.011161
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Urinary excretion of the low molecular weight protein cystatin C is a marker of renal disorders and a good predictor of the severity of acute tubular necrosis. We evaluated the measurement of urinary cystatin C and determined its reference range. METHODS:Measurement of urinary cystatin C by particle-enhance...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456304322879980
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:Macroprolactin is a high molecular mass complex of prolactin that may be the cause of elevated serum prolactin as determined by immunoassay. The composition of macroprolactin and its reactivity in immunoassays are variable but the complex has minimal bioactivity in vivo. Hyperprolactinaemia due to unrecogniz...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306775141830
更新日期:2006-01-01 00:00:00
abstract::A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) m...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329202900606
更新日期:1992-11-01 00:00:00
abstract::We conducted an electrophoretic analysis of monoclonal gamma-globulin found in the serum of a patient with splenic marginal-zone lymphoma. This monoclonal protein showed electrophoretic mobility to the gamma region and reacted with anti-immunoglobulin (IgG) antiserum but not with anti-kappa or anti-lambda light chain ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/000456306776021490
更新日期:2006-03-01 00:00:00
abstract::It is our aim to summarise and discuss procedures for the evaluation of the concentration of free ionised calcium in serum or plasma. Stress is laid upon the interrelations and relative validity of the most common algebraic expressions to appraise the calcium status. The multitude of formulae proposed in the literatur...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1177/000456328402100501
更新日期:1984-09-01 00:00:00
abstract::Background Measured (MO) and calculated osmotic concentrations (CO) and the osmotic gap (OG) are commonly used in the investigation of electrolyte and volume disturbances as well as in cases of suspected volatile ingestion. Methods We compared 38 published formulae for CO with MO on a large data-set ( n = 9466) and ad...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563217694052
更新日期:2018-01-01 00:00:00
abstract::The application of a DC plasma emission spectrometer to the measurement of copper, zinc and magnesium in serum and urine is reported. Each assay requires only a simple dilution in 1% nitric acid. Comparisons with standard atomic absorption techniques showed good analytical agreement, especially for copper and magnesiu...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456328502200512
更新日期:1985-09-01 00:00:00
abstract:BACKGROUND:Oxidative stress may play an aetiological role in the development and progression of cardiovascular disease (CVD). However, evidence on its biochemical markers has been controversial. This article aimed to assess the role of F2-isoprostanes, a marker for measuring in vivo lipid oxidation, as a biomarker for ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1258/acb.2012.011263
更新日期:2013-03-01 00:00:00
abstract::Smoking is a major risk factor for periodontal disease, causing bone and tooth loss. Effective management of many oral conditions requires accurate information about smoking. We describe a disposable, 10-min, near-patient saliva test to monitor cigarette smoking. A plastic device contains the dried reagents to measure...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1258/0004563001899717
更新日期:2000-09-01 00:00:00
abstract::Background Analysis of dried blood specimens has been an integral part of laboratory medicine dating back to the early 1960s when they were introduced as part of neonatal screening programs. More recently, they have been used in Down syndrome screening programmes. Dried blood spot specimens can be collected either by ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/0004563213484906
更新日期:2013-11-01 00:00:00
abstract::We developed a binary gradient high-performance liquid chromatography (HPLC) method for measuring HbA2 in whole blood samples using a Pharmacia Mono Q column (1 mL) and measurement at 415 nm. The assay requires a simple lysis and centrifugation step before injection onto the column. We found good agreement of results ...
journal_title:Annals of clinical biochemistry
pub_type: 杂志文章
doi:10.1177/000456329603300313
更新日期:1996-05-01 00:00:00
abstract:BACKGROUND:Refeeding syndrome (RS) is a potentially fatal condition that can occur following the re-introduction of nutrition after a period of starvation. Hypophosphataemia following the reintroduction of nutrition is often the only reliable biochemical marker of RS. Refeeding index (RI) generated from baseline insuli...
journal_title:Annals of clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1177/0004563214523739
更新日期:2015-01-01 00:00:00