High-performance liquid chromatography methods for the analysis of endogenous cortisol and cortisone in human urine: comparison of mass spectrometry and fluorescence detection.

abstract：:The effects of inhibition of inosine monophosphate dehydrogenase (IMPDH), the rate-limiting enzyme in guanine nucleotide de novo synthesis, on cell growth, cell viability, endogenous nucleotide concentrations and concentrations of extracellular nucleosides and bases were studied in Molt F4 human malignant lymphoblasts...

journal_title：Annals of clinical biochemistry

authors： Stet EH,De Abreu RA,Bökkerink JP,Lambooy LH,Vogels-Mentink TM,Keizer-Garritsen JJ,Trijbels FJ

更新日期：1994-03-01 00:00:00

abstract：:The concentrations of serum calcium, parathyroid hormone (PTH), 25 Hydroxyvitamin D (25OHD), and 1,25 Dihydroxyvitamin D (1,25(OH)2D) were determined in 99 Saudi patients with sickle cell disease and in 104 matching healthy controls. Serum calcium and 25OHD were significantly lower in the patients, with 14% and 12% of...

journal_title：Annals of clinical biochemistry

authors： Mohammed S,Addae S,Suleiman S,Adzaku F,Annobil S,Kaddoumi O,Richards J

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:Angiogenesis is involved in the pathogenesis of diabetic retinopathy. Osteoprotegerin, a recently identified glycoprotein belonging to the tumour necrosis factor receptor superfamily, has been implicated to be correlated with angiogenesis. This study aims to determine whether serum and vitreous concentration...

journal_title：Annals of clinical biochemistry

authors： Yu G,Ji X,Jin J,Bu S

更新日期：2015-03-01 00:00:00

abstract：:Whole blood and washed erythrocytes from nutritionally normal and abnormal elderly patients were stored at room temperature, 4 degrees C and -20 degrees C for up to 14 days. In both groups, and under all storage conditions, the erythrocyte transketolase (ETK) activity and the thiamine pyrophosphate (TPP) effect decrea...

journal_title：Annals of clinical biochemistry

authors： Puxty JA,Haskew AE,Ratcliffe JG,McMurray J

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:Circulating levels of bioavailable oestradiol and testosterone are often desirable for clinical practice or investigational studies of children. However, assays to measure circulating hormone levels might not always be accessible. We sought to validate the empirical calculation of circulating bioavailable te...

journal_title：Annals of clinical biochemistry

authors： Egleston BL,Chandler DW,Dorgan JF

更新日期：2010-05-01 00:00:00

abstract：:A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the ...

journal_title：Annals of clinical biochemistry

authors： Brown K,Gardner JJ,Lockley WJ,Preston JR,Wilkinson DJ

更新日期：1983-01-01 00:00:00

abstract：:An automated assay for serum amylase is described. Using the AutoAnalyzer II the method employs, as substrate, a solution of dyed amylopectin which is commercially available (DyAmyl-L). After incubation of serum and substrate at 55 degrees C, 1.0 mol/lKOH is introduced and the coloured products of enzymic hydrolysis a...

journal_title：Annals of clinical biochemistry

authors： Carney JA,Osibeluwo SA,Clements JA,Jay RF

更新日期：1977-11-01 00:00:00

abstract：:The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...

journal_title：Annals of clinical biochemistry

authors： Simkins A,Worth HG

更新日期：1976-01-01 00:00:00

abstract：BACKGROUND:With the recent outbreak in West Africa, hospitals worldwide have been developing protocols for suspect of cases of Ebola virus disease. Patients with Ebola virus disease present with a severe gastroenteritis leading to dehydration and electrolyte abnormalities and as such, routine chemistry analysis is esse...

journal_title：Annals of clinical biochemistry

authors： LeBlanc JJ,Heinstein C,MacDonald J,Gallant R,Roberts C,Jackson C,Lou A,Nassar BA,Hatchette TF

更新日期：2016-03-01 00:00:00

abstract：:γ-glutamyltransferase plays a key role in the synthesis and metabolism of extracellular glutathione, a major antioxidant in several defence mechanisms in the body. γ-glutamyltransferase is affected by environmental and genetic factors, and is raised when there is depletion of glutathione. Hence, it is a marker of oxid...

journal_title：Annals of clinical biochemistry

authors： Bulusu S,Sharma M

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:Patients with mutations of succinate dehydrogenase B (SDHB) and succinate dehydrogenase D (SDHD) are at high risk of paraganglioma necessitating surveillance. Chromogranin A has been proposed as a biochemical marker of paraganglioma. We sought to determine the diagnostic utility of chromogranin A in a popula...

journal_title：Annals of clinical biochemistry

authors： Thompson MJ,Parameswaran V,Burgess JR

更新日期：2019-01-01 00:00:00

abstract：:Experience in the use of an in-house urinary free catecholamine assay for the investigation of possible neural crest tumours in a District General Hospital laboratory is described. Elevated excretion of catecholamines and vanillylmandelic acid was found in a number of cases including phaeochromocytoma and neuroblastom...

journal_title：Annals of clinical biochemistry

authors： Davidson DF

更新日期：1987-09-01 00:00:00

abstract：:Pancreatic elastase 1 (E1), a digestive protease, is synthesized by the acinar cells of the pancreas. Using an enzyme-linked immunosorbent assay, we evaluated stool E1 levels in the following groups of patients. (a) Specimens submitted for occult blood examination from 20 adults, over 3 consecutive days, to assess the...

journal_title：Annals of clinical biochemistry

authors： Phillips IJ,Rowe DJ,Dewar P,Connett GJ

更新日期：1999-11-01 00:00:00

abstract：BACKGROUND:Supported liquid extraction (SLE) techniques are relatively new compared to other sample preparation approaches such as solid phase extraction (SPE), liquid-liquid extraction (LLE) and protein precipitation (PPE). We investigated the use of SLE as an alternative to SPE for the liquid chromatography tandem ma...

journal_title：Annals of clinical biochemistry

authors： Owen LJ,Keevil BG

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Biological variation of an analyte is an important determinant of its usefulness. Intraindividual variation in serum thyroid hormones, parathyroid hormone (PTH) and insulin-like growth factor-1 (IGF-1) in healthy subjects was determined in this study. METHODS:Blood samples were taken at weekly intervals for...

journal_title：Annals of clinical biochemistry

authors： Ankrah-Tetteh T,Wijeratne S,Swaminathan R

更新日期：2008-03-01 00:00:00

abstract：BACKGROUND:Emergency abdominal surgery carries considerable postoperative morbidity and mortality. Hypovolaemia is considered to be a cause of renal hypoperfusion, which is associated with a decreased clearance of serum urea and creatinine. This study examines whether the perioperative serum urea and creatinine concent...

journal_title：Annals of clinical biochemistry

authors： Harten J,Hay A,McMillan DC,McArdle CS,O'Reilly DS,Kinsella J

更新日期：2006-07-01 00:00:00

abstract：:Antibodies to glutamic acid decarboxylase (GAD) are a useful autoimmune marker for type 1 diabetes mellitus in Caucasians. We examined antibodies to GAD and their relationships with clinical features and pancreatic beta cell function in 140 young Chinese diabetic patients. Over an 18-month period beginning in 1995, 14...

journal_title：Annals of clinical biochemistry

authors： Ko GT,Chan JC,Yeung VT,Chow CC,Li JK,Lau MS,Mackay IR,Rowley MJ,Zimmet P,Cockram CS

更新日期：1998-11-01 00:00:00

abstract：:The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient...

journal_title：Annals of clinical biochemistry

authors： Trewick AL

更新日期：1998-03-01 00:00:00

abstract：BACKGROUND:Oxidation of lipoproteins is thought to play a crucial role in atherogenesis. Role for triglyceride-rich lipoproteins in atherogenesis is unclear. Thus, we aimed to investigate whether cholesteryl ester hydroperoxides (CEOOH) are present in very low-density lipoproteins (VLDL) and intermediate-density lipopr...

journal_title：Annals of clinical biochemistry

authors： Shrestha R,Hui SP,Sakurai T,Yagi A,Takahashi Y,Takeda S,Jin S,Fuda H,Chiba H

更新日期：2014-11-01 00:00:00

abstract：:Rabbit uterus contains type I and II oestrogen binding sites in the cytosol, and nuclear fractions. Oestrogen-stimulated increase in uterine weight was inhibited by concurrent treatment with progesterone or danazol. Oestrogen-induced type I binding sites were decreased by progesterone and to a lesser extent by danazol...

journal_title：Annals of clinical biochemistry

authors： Tamaya T,Wada K,Mori H,Imai A

更新日期：1991-05-01 00:00:00

abstract：BACKGROUND:In humans, leptin circulates in a free form and is also bound to macromolecules. The aim of the present study was to compare a rapid acid-ethanol precipitation (AEP) method of measuring bound leptin with the more laborious gel exclusion chromatography (GEC) reference procedure. Serum samples collected from h...

journal_title：Annals of clinical biochemistry

authors： Jamieson NB,McMillan DC,Brown DJ,Wallace AM

更新日期：2003-03-01 00:00:00

abstract：BACKGROUND:Pharmacometabonomics is a new branch of science, first described in 2006 and defined as 'the prediction of the effects of a drug on the basis of a mathematical model of pre-dose metabolite profiles'. Pharmacometabonomics has been used to predict drug metabolism, pharmacokinetics (PK), drug safety and drug ef...

journal_title：Annals of clinical biochemistry

authors： Everett JR,Loo RL,Pullen FS

更新日期：2013-11-01 00:00:00

abstract：:The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...

journal_title：Annals of clinical biochemistry

authors： Leijten JF,van der Geer F,Scholten MN,Goldschmidt HM

更新日期：1984-03-01 00:00:00

abstract：BACKGROUND:Current guidelines document persistent rectal bleeding as an alarm symptom in patients presenting to primary care. We studied whether a faecal immunochemical test could assist in their assessment. METHODS:From December 2015, faecal immunochemical tests were routinely available to primary care when assessing...

journal_title：Annals of clinical biochemistry

authors： Digby J,Strachan JA,McCann R,Steele RJ,Fraser CG,Mowat C

更新日期：2020-07-01 00:00:00

abstract：:The immunosuppressive agent mycophenolate mofetil is a prodrug for the active compound mycophenolic acid. Following organ transplantation, it is used mostly in combination with other immunosuppressive drugs, but it has also been used as primary therapy. Although in the pivotal clinical studies the drug was given in fi...

journal_title：Annals of clinical biochemistry

authors： Holt DW

更新日期：2002-05-01 00:00:00

abstract：BACKGROUND:Calculated globulin fraction is derived from the liver function tests by subtracting albumin from the total protein. Since immunoglobulins comprise the largest component of the serum globulin concentration, increased or decreased calculated globulins and may identify patients with hypogammaglobulinaemia or h...

journal_title：Annals of clinical biochemistry

authors： Hoo T,Lim E,John M,D'Orsogna L,McLean-Tooke A

更新日期：2021-01-11 00:00:00

abstract：BACKGROUND:In the enzyme reaction for the determination of the unbound (free) bilirubin concentration by glucose oxidase and peroxidase, materials with low affinity for serum protein are reactive. The influence of these materials on the determination of serum unbound bilirubin was investigated. METHODS Serum samples fr...

journal_title：Annals of clinical biochemistry

authors： Itoh S,Kawada K,Kusaka T,Yasuda S,Okada H,Imai T,Isobe K

更新日期：2002-11-01 00:00:00

abstract：BACKGROUND:Recent NICE Guidelines have emphasized the need to have in place by 2007 the capability of offering screening to all women in the first trimester using a combination of maternal age with the ultrasound marker nuchal translucency thickness (NT) and the maternal serum biochemical markers free beta-hCG and preg...

journal_title：Annals of clinical biochemistry

authors： Spencer K

更新日期：2005-01-01 00:00:00

abstract：:We report a 63-year-old man who presented with breathlessness and weight loss and was diagnosed on echocardiography to have carcinoid heart disease. It was later transpired that he underwent surgical removal of an ileal carcinoid tumour 19 years previously. ...

journal_title：Annals of clinical biochemistry

authors： Sulaiman R,Panting M,Bhogal G,Perry I,Ford C,Gama R

更新日期：2010-03-01 00:00:00

abstract：:Free and total reduced concentrations of D-penicillamine have been measured in the plasma of rheumatoid arthritis patients by HPLC and electro-chemical detection. A reverse-phase ion-pair separation in conjunction with a dual porous graphite electrode satisfied the requirements of robustness, sensitivity, selectivity ...

journal_title：Annals of clinical biochemistry

authors： Webb BA,Richardson SJ,Haslock I

更新日期：1988-03-01 00:00:00